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Identification Of The MerR Gene Of R100 By Using Mer-lac Gene And Operon Fusions.

T. Foster, N. Brown
Published 1985 · Biology, Medicine

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Transcriptional (operon) and translational (gene) fusions between the R100 merR gene and lacZ were constructed in vitro in a pBR322 plasmid carrying the mer genes derived from plasmid R100. The translational fusions were oriented in the opposite direction to and divergently from the merTCAD genes. This shows that the reading frame previously thought to be merR was incorrect. Expression of the gene fusion was repressed in trans by a compatible plasmid carrying the R100 merR+ gene, as was a similarly oriented transcriptional fusion. In contrast, expression of beta-galactosidase by the lac fragment located at the same site but in the opposite orientation was at a lower level and was not repressed by merR+.



This paper is referenced by
10.1016/0147-619X(88)90013-3
Restriction pattern and polypeptide homology among plasmid-borne mercury resistance determinants.
M. Jobling (1988)
10.1128/JB.171.8.4241-4247.1989
Mercury operon regulation by the merR gene of the organomercurial resistance system of plasmid pDU1358.
G. Nucifora (1989)
Genetic systems encoding heavy metal resistance and/or transport in Achromobacter sp. strain AO22 and their applications in environmental biotechnology
Shee Ping Ng (2010)
10.1016/j.csbj.2020.12.006
Recent advances in exploring the heavy metal(loid) resistant microbiome
Xiuli Hao (2021)
10.1002/AOC.590030402
Mercury resistance and detoxification in bacteria
B. H. Belliveau (1989)
10.1016/0147-619X(92)90002-R
Bacterial resistances to inorganic mercury salts and organomercurials.
T. Misra (1992)
10.1016/S0014-5793(00)01430-7
MerF is a mercury transport protein: different structures but a common mechanism for mercuric ion transporters?
J. Wilson (2000)
10.1016/0921-4526(89)90221-4
XAS of the MerR metalloregulatory protein
J. Penner-Hahn (1989)
10.1099/00221287-144-10-2855
Selection and characterization of mercury-independent activation mutants of the Tn501 transcriptional regulator, MerR.
J. Parkhill (1998)
"Prokaryotic Metallothionein gene isolation, Nucleotide sequence and expression"
J. Huckle (1993)
10.1046/j.1365-2958.2001.02264.x
CueR (YbbI) of Escherichia coli is a MerR family regulator controlling expression of the copper exporter CopA
J. V. Stoyanov (2001)
10.1016/S0168-6445(03)00049-4
Escherichia coli mechanisms of copper homeostasis in a changing environment.
C. Rensing (2003)
10.1007/BF00260858
Down regulation of the mercury resistance operon by the most promoter-distal gene merD
G. Nucifora (2004)
10.1007/978-94-011-3456-9_18
Bacterial Heavy Metal Resistance Systems and Possibility of Bioremediation
S. Silver (1991)
10.1126/SCIENCE.3798107
Metalloregulatory DNA-binding protein encoded by the merR gene: isolation and characterization.
T. O'Halloran (1987)
10.1002/9780470166390.CH6
Mercury(II)-thiolate chemistry and the mechanism of the heavy metal biosensor MerR
J. G. Wright (1990)
10.1099/00221287-132-2-465
Transcriptional regulation of the mercury-resistance genes of transposon Tn501.
P. Lund (1986)
10.1111/j.1365-2958.1991.tb01979.x
The merR regulatory gene in Thiobacillus ferrooxidans is spaced apart from the mer structural genes
C. Inoue (1991)
10.1016/0141-0229(90)90022-I
Kinetics of mercuric reduction in intact and permeabilized Escherichia coli cells
G. Philippidis (1990)
10.1002/BIT.260370108
A model for mercuric ion reduction in recombinant Escherichia coli
G. Philippidis (1991)
10.3109/10408418709104455
The genetics and biochemistry of mercury resistance.
T. Foster (1987)
Potential of plant genetic engineering for phytoremediation of toxic trace elements
N. Verbruggen (2009)
10.1016/0378-1119(94)90835-4
The sequence of the mer operon of pMER327/419 and transposon ends of pMER327/419, 330 and 05.
J. Hobman (1994)
10.1021/BI00493A011
Activator-dependent preinduction binding of sigma-70 RNA polymerase at the metal-regulated mer promoter.
A. Heltzel (1990)
10.1007/BF02917247
DNA sequence analysis of bacterial toxic heavy metal resistances
S. Silver (2008)
10.1007/BF00330531
The nucleotide sequence of the mercuric resistance operons of plasmid R100 and transposon Tn501: further evidence for mer genes which enhance the activity of the mercuric ion detoxification system
N. Brown (2004)
10.1007/BF01055908
Toxic element contamination and the occurrence of mercury-resistant bacteria in Hg-Contaminated soil, sediments, and sludges
B. Olson (1991)
Biological Mercury Reduction in the Environment and Its Policy Implications for Metals Regulations Based on Speciation
J. Hogan-Sheldon (1995)
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