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Overproduction Of Escherichia Coli Integration Host Factor, A Protein With Nonidentical Subunits.

H. Nash, C. Robertson, E. Flamm, R. Weisberg, H. I. Miller
Published 1987 · Medicine, Biology

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Integration host factor (IHF) is a small, basic protein that is needed for efficient recombination of bacteriophage lambda, as well as for other host and viral functions. We have constructed strains in which the two subunits of IHF, encoded by the himA and hip genes of Escherichia coli, are expressed under the control of the lambda rho L promoter. Separate overexpression of himA and hip led to the production of unstable and insoluble peptides, respectively. In contrast, the overexpression of both genes conjointly led to the accumulation of large amounts of active IHF. Extracts of such cells provided the starting material for a rapid purification procedure that results in milligram quantities of apparently homogeneous IHF.

This paper is referenced by
Proteases and protein degradation in Escherichia coli.
Maurizi Mr (1992)
Effect of Escherichia coli IHF mutations on plasmid p15A copy number.
E. Hiszczyńska-Sawicka (1997)
Specific and non-specific interactions of integration host factor with DNA: thermodynamic evidence for disruption of multiple IHF surface salt-bridges coupled to DNA binding.
J. Holbrook (2001)
The interaction of E. coli IHF protein with its specific binding sites
Chien-Chin Yang (1989)
The Escherichia coli K-12 NarL and NarP proteins insulate the nrf promoter from the effects of integration host factor.
D. Browning (2006)
Integration Host Factor is Required for the Induction of Acid Resistance in Escherichia coli
Hongkai Bi (2014)
Transcription activation by remodelling of a nucleoprotein assembly: the role of NarL at the FNR‐dependent Escherichia coli nir promoter
D. Browning (2004)
Transcription activation by histone-like protein integration host factor.
N. Goosen (1996)
Cloning, Expression, and Biochemical Characterization of Hexahistidine-tagged Terminase Proteins*
Q. Hang (1999)
Stepwise binding and bending of DNA by Escherichia coli integration host factor
Sawako Sugimura (2006)
Escherichia coli Operon in astCADBE ArgR-Dependent Superinduction of the ArgR-Independent Induction and
A. K. Kiupakis (2001)
Regulation by nucleoid-associated proteins at the Escherichia coli nir operon promoter.
D. Browning (2008)
Response Regulator Heterodimer Formation Controls a Key Stage in Streptomyces Development
Mahmoud M. Al-Bassam (2014)
Activation of Gene Expression by a Novel DNA Structural Transmission Mechanism That Requires Supercoiling-induced DNA Duplex Destabilization in an Upstream Activating Sequence*
S. Sheridan (1998)
ArgR-independent induction and ArgR-dependent superinduction of the astCADBE operon in Escherichia coli.
A. K. Kiupakis (2002)
Reconstitution of F Factor DNA Replication in Vitro with Purified Proteins*
S. Zzaman (2004)
Integration host factor positively regulates virulence gene expression in Vibrio cholerae.
E. Stonehouse (2008)
Two roles for integration host factor at an enhancer‐dependent nifA promoter
R. Wassem (2000)
Indirect Recognition in Sequence-specific DNA Binding by Escherichia coli Integration Host Factor
Kimberly A. Aeling (2006)
Growth phase-dependent regulation of nuoA-N expression in Escherichia coli K-12 by the Fis protein: upstream binding sites and bioenergetic significance
B. Wackwitz (1999)
The λ Integrase Site-speci fi c Recombination Pathway
A. Landy (2015)
Mapping and regulation of the pifC promoter of the F plasmid.
M. Kennedy (1988)
The Mu transpositional enhancer can function in trans: Requirement of the enhancer for synapsis but not strand cleavage
M. Surette (1992)
Aberrant Community Architecture and Attenuated Persistence of Uropathogenic Escherichia coli in the Absence of Individual IHF Subunits
S. Justice (2012)
DNABII proteins play a central role in UPEC biofilm structure
A. Devaraj (2015)
Kinetic characterization of the ATPase activity of the DNA packaging enzyme from bacteriophage lambda.
M. Tomka (1993)
Contrasting Enzymatic Activities of Topoisomerase IV and DNA Gyrase from Escherichia coli*
C. Ullsperger (1996)
Substrate recognition and induced DNA deformation by transposase at the target-capture stage of Tn10 transposition.
P. A. Pribil (2000)
The expression of nifB gene from Herbaspirillum seropedicae is dependent upon the NifA and RpoN proteins.
F. Rego (2006)
Final Report DOE Grant # DE-FG 03-02 ER 63339 November 10 , 2004
S. Weiss (2004)
Specific and nonspecific interactions of integration host factor with oligo DNAs as revealed by circular dichroism spectroscopy and filter binding assay.
H. Kurumizaka (1992)
Stimulation of the phage λ pL promoter by integration host factor requires the carboxy terminus of the α-subunit of RNA polymerase
H. Giladi (1992)
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