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Localization Of Periplasmic Redox Proteins OfAlcaligenes Faecalis By A Modified General Method For Fractionating Gram-Negative Bacteria

Zhenyu Zhu, Dapeng Sun, Victor L. Davidson

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ABSTRACTA lysozyme-osmotic shock method is described for fractionation ofAlcaligenes faecaliswhich uses glucose to adjust osmotic strength and multiple osmotic shocks. During phenylethylamine-dependent growth, aromatic amine dehydrogenase, azurin, and a single cytochromecwere localized in the periplasm. Their induction patterns are different from those for the related quinoprotein methylamine dehydrogenase and its associated redox proteins.