Please confirm you are human (Sign Up for free to never see this)
← Back to Search
Multicenter Evaluation Of Fully Automated BACTEC Mycobacteria Growth Indicator Tube 960 System For Susceptibility Testing Of Mycobacterium Tuberculosis
P. Bémer, F. Palicova, S. Rüsch-Gerdes, H. Drugeon, G. Pfyffer
Published 2002 · Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
ABSTRACT The reliability of the BACTEC Mycobacteria Growth Indicator Tube (MGIT) 960 system for testing of Mycobacterium tuberculosis susceptibility to the three front-line drugs (isoniazid [INH], rifampin [RIF], and ethambutol [EMB]) plus streptomycin (STR) was compared to that of the BACTEC 460 TB system. The proportion method was used to resolve discrepant results by an independent arbiter. One hundred and ten strains were tested with an overall agreement of 93.5%. Discrepant results were obtained for seven strains (6.4%) with INH (resistant by BACTEC MGIT 960; susceptible by BACTEC 460 TB), for one strain (0.9%) with RIF (resistant by BACTEC MGIT 960; susceptible by BACTEC 460 TB), for seven strains (6.4%) with EMB (six resistant by BACTEC MGIT 960 and susceptible by BACTEC 460 TB; one susceptible by BACTEC MGIT 960 and resistant by BACTEC 460 TB), and for 19 strains (17.3%) with STR (resistant by BACTEC MGIT 960 and susceptible by BACTEC 460 TB). After resolution of discrepant results, the sensitivity of the BACTEC MGIT 960 system was 100% for all four drugs and specificity ranged from 89.8% for STR to 100% for RIF. Turnaround times were 4.6 to 11.7 days (median, 6.5 days) for BACTEC MGIT 960 and 4.0 to 10.0 days (median, 7.0 days) for BACTEC 460 TB. These data demonstrate that the fully automated and nonradiometric BACTEC MGIT 960 system is an accurate method for rapid susceptibility testing of M. tuberculosis.
This paper references
Mycobacterial growth indicator tube for susceptibility testing of Mycobacterium tuberculosis to isoniazid and rifampin.
J. S. Bergmann (1997)
Testing of susceptibility of Mycobacterium tuberculosis to isoniazid and rifampin by mycobacterium growth indicator tube method.
S. Walters (1996)
Evaluation of Mycobacteria Growth Indicator Tube for Direct and Indirect Drug Susceptibility Testing ofMycobacterium tuberculosis from Respiratory Specimens in a Siberian Prison Hospital
V. Goloubeva (2001)
Multicenter Evaluation of the Mycobacteria Growth Indicator Tube for Testing Susceptibility of Mycobacterium tuberculosis to First-Line Drugs
S. Rüsch-Gerdes (1999)
Rapid susceptibility testing of Mycobacterium tuberculosis by the Mycobacteria Growth Indicator Tube (MGIT AST SIRE).
E. Macondo (2000)
Empfindlichkeitsprüfung von Tuberkulosebakterien gegen Chemotherapeutika. Norm 58943-8
Evaluation of rapid radiometric method for drug susceptibility testing of Mycobacterium tuberculosis.
S. Siddiqi (1981)
Anti-tuberculosis drug resistance in the world: The WHO/IUATLD Global Project on Anti-Tuberculosis Drug Resistance Surveillance. Geneva: World Health Organization, 1997. 228pp. Price Sw. fr 25/US$22.50 (in developing countries Sw. fir 17.50). (Reference WHO/TB/97.229)
D. Wilkinson (1998)
Evaluation of the BACTEC radiometric method for recovery of mycobacteria and drug susceptibility testing of Mycobacterium tuberculosis from acid-fast smear-positive specimens.
G. Roberts (1983)
Evaluation of Mycobacteria Growth Indicator Tube (MGIT) for drug susceptibility testing of Mycobacterium tuberculosis.
J. Palomino (1999)
Reliability of the MB/BacT System for Testing Susceptibility of Mycobacterium tuberculosis Complex Isolates to Antituberculous Drugs
F. Brunello (2000)
Quality assurance programme for drug susceptibility testing of Mycobacterium tuberculosis in the WHO/IUATLD Supranational Laboratory Network: first round of proficiency testing.
A. Laszlo (1997)
An outbreak of tuberculosis caused by multiple-drug-resistant tubercle bacilli among patients with HIV infection.
M. Fischl (1992)
Evaluation of Mycobacteria Growth Indicator Tube (MGIT) for drug susceptibility testing VOL
J C Palomino (1999)
Comparison of the mycobacteria growth indicator tube with radiometric and solid culture for isolation of mycobacteria from clinical specimens and susceptibility testing of Mycobacterium tuberculosis.
F. Ardito (2000)
Evaluation of mycobacteria growth indicator tube for recovery and drug susceptibility testing of Mycobacterium tuberculosis isolates from respiratory specimens.
M. Palací (1996)
Evaluation of the ESP Culture System II for Testing Susceptibilities of Mycobacterium tuberculosis Isolates to Four Primary Antituberculous Drugs
J. S. Bergmann (1998)
J. CLIN. MICROBIOL
Susceptibility testing of Mycobacteria, Nocardia, and other aerobic Actinomycetes. Tentative standard
Anti-tuberculosis drug resistance in the world : the WHO/IUATLD Global Project on Anti-tuberculosis Drug Resistance Surveillance
Global Tuberculosis Programme (1997)
Evaluation of the MB/BacT Mycobacterium Detection System for Susceptibility Testing of Mycobacterium tuberculosis
M. Díaz-Infantes (2000)
This paper is referenced by
Pruebas de sensibilidad para Mycobacterium tuberculosis
C. Ugarte-Gil (2008)
Delamanid susceptibility testing of Mycobacterium tuberculosis using the resazurin microtitre assay and the BACTEC™ MGIT™ 960 system.
E. Schena (2016)
Rapid drug susceptibility test of Mycobacterium tuberculosis using microscopic time-lapse imaging in an agarose matrix
Jungil Choi (2015)
Detection and drug-susceptibility testing of M. tuberculosis from sputum samples using luciferase reporter phage: comparison with the Mycobacteria Growth Indicator Tube (MGIT) system.
S. Bardarov (2003)
Drug resistance of Mycobacterium tuberculosis complex in central Taiwan.
C. Liu (2004)
Multidrug-Resistant Tuberculosis Drug Susceptibility and Molecular Diagnostic Testing
Ameeta S Kalokhe (2013)
Comparison of the mycobacterium growth indicator tube method and the method of proportion for drug susceptibility testing of mycobacterium tuberculosis.
A. E. Aktaş (2014)
A. McCullough (2018)
Clinical Management of Multidrug‐Resistant Tuberculosis
S. Shin (2017)
Evaluation of the Fully Automated BACTEC MGIT 960 System for Testing Susceptibility of Mycobacterium tuberculosis to Pyrazinamide, Streptomycin, Isoniazid, Rifampin, and Ethambutol and Comparison with the Radiometric BACTEC 460TB Method
C. Scarparo (2004)
Susceptibility testing to second-line drugs and ethambutol by GenoType MTBDRsl and Bactec MGIT 960 comparing with agar proportion method.
P. López-Roa (2012)
L. D'Ambrosio (2012)
High Treatment Success Rates Among HIV-Infected Multidrug-Resistant Tuberculosis Patients After Expansion of Antiretroviral Therapy in Botswana, 2006–2013
S. Shin (2017)
Indigenous Transmission of Mycobacterium africanum in Canada: A Case Series and Cluster Analysis
A. Chakravarti (2019)
KAPITEL C3 – Tuberkulose
Gabriela Pfyffer von Altishofen (2008)
The menace of tuberculosis and the role of nuclear medicine in tackling it. Now its time to tighten the loose strings.
V. Gupta (2009)
Rapid detection and characterization of mycobacteria using microchannel electrical impedance spectroscopy
Roli Kargupta (2017)
An Integrated Approach to Rapid Diagnosis of Tuberculosis and Multidrug Resistance Using Liquid Culture and Molecular Methods in Russia
Y. Balabanova (2009)
Multicenter research on the BACTEC MGIT 960 system for the second-line drugs susceptibility testing of Mycobacterium tuberculosis in China.
L. Zhao (2013)
An update on pathogenesis and management of tuberculosis with special reference to drug resistance
S. K. Bhunia (2015)
Mycobacterium tuberculosis Complex, Mycobacterium leprae, and Other Slow‐Growing Mycobacteria
G. Pfyffer (2010)
Susceptibility Testing with the Manual Mycobacteria Growth Indicator Tube (MGIT) and the MGIT 960 System Provides Rapid and Reliable Verification of Multidrug-Resistant Tuberculosis
Kati Ådjers-Koskela (2003)
Rapid methods for testing inhibitors of mycobacterial growth.
D. Evangelopoulos (2010)
Laboratory diagnosis of tuberculosis: novel and nonconventional methods
A. Somoskovi (2008)
Comparison between the BACTEC MGIT 960 system and the agar proportion method for susceptibility testing of multidrug resistant tuberculosis strains in a high burden setting of South Africa
H. Said (2012)
A Comparison of Tools Used for Tuberculosis Diagnosis in Resource-Limited Settings: A Case Study at Mubende Referral Hospital, Uganda
A. Muwonge (2014)
Antimicrobial susceptibility testing of Mycobacterium tuberculosis (EUCAST document E.DEF 8.1)--report of the Subcommittee on Antimicrobial Susceptibility Testing of Mycobacterium tuberculosis of the European Committee for Antimicrobial Susceptibility Testing (EUCAST) of the European Society of Clin
F. Drobniewski (2007)
Diagnostic test of rifampicin resistance in mycobacterium tuberculosis: a meta‐analysis
Bin Ma (2011)
Multicenter Laboratory Validation of the BACTEC MGIT 960 Technique for Testing Susceptibilities of Mycobacterium tuberculosis to Classical Second-Line Drugs and Newer Antimicrobials
S. Rüsch-Gerdes (2006)
Usefulness of the GenoType MTBC Assay for Differentiating Species of the Mycobacterium tuberculosis Complex in Cultures Obtained from Clinical Specimens
E. Richter (2004)
Isoniazid Mono-Resistant Tuberculosis: Impact on Treatment Outcome and Survival of Pulmonary Tuberculosis Patients in Southern Mexico 1995-2010
R. Báez-Saldaña (2016)
Pyrazinamide resistance determined by liquid culture at low pH better correlates with genetic mutations in MDR tuberculosis isolates.
Y. Pang (2015)See more