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Characterization Of RAD51-Independent Break-Induced Replication That Acts Preferentially With Short Homologous Sequences

G. Ira, J. Haber
Published 2002 · Biology, Medicine

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ABSTRACT Repair of double-strand breaks by gene conversions between homologous sequences located on different Saccharomyces cerevisiae chromosomes or plasmids requires RAD51. When repair occurs between inverted repeats of the same plasmid, both RAD51-dependent and RAD51-independent repairs are found. Completion of RAD51-independent plasmid repair events requires RAD52, RAD50, RAD59, TID1 (RDH54), and SRS2 and appears to involve break-induced replication coupled to single-strand annealing. Surprisingly, RAD51-independent recombination requires much less homology (30 bp) for strand invasion than does RAD51-dependent repair (approximately 100 bp); in fact, the presence of Rad51p impairs recombination with short homology. The differences between the RAD51- and RAD50/RAD59-dependent pathways account for the distinct ways that two different recombination processes maintain yeast telomeres in the absence of telomerase.
This paper references
Meiotic recombination initiated by a double-strand break in rad50 delta yeast cells otherwise unable to initiate meiotic recombination.
A. Malkova (1996)
10.1126/SCIENCE.1411547
Removal of nonhomologous DNA ends in double-strand break recombination: the role of the yeast ultraviolet repair gene RAD1.
J. Fishman-Lobell (1992)
10.1074/jbc.C000133200
The Relationship between Homology Length and Crossing Over during the Repair of a Broken Chromosome*
O. Inbar (2000)
10.1073/pnas.061579598
Recombination-mediated lengthening of terminal telomeric repeats requires the Sgs1 DNA helicase
H. Cohen (2001)
Rad52 forms ring structures and cooperates with RPA in single-strand DNA annealing. Genes Cells 3:145–156
A. Shinohara (1998)
10.1046/j.1365-2443.1998.00176.x
Rad52 forms ring structures and co‐operates with RPA in single‐strand DNA annealing
A. Shinohara (1998)
10.1101/GAD.826100
Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1.
G. Petukhova (2000)
10.1073/PNAS.93.14.7131
Double-strand break repair in the absence of RAD51 in yeast: a possible role for break-induced DNA replication.
A. Malkova (1996)
RAD50 and RAD51 define two pathways that collaborate to maintain telomeres in the absence of telomerase.
S. Le (1999)
10.1128/MCB.20.24.9162-9172.2000
Aberrant Double-Strand Break Repair inrad51 Mutants of Saccharomyces cerevisiae
L. Kang (2000)
10.1074/jbc.274.41.29453
Yeast Rad54 Promotes Rad51-dependent Homologous DNA Pairing via ATP Hydrolysis-driven Change in DNA Double Helix Conformation*
G. Petukhova (1999)
10.1073/pnas.151008198
Break-induced replication: A review and an example in budding yeast
E. Kraus (2001)
10.1074/jbc.271.52.33181
Direct Association between the Yeast Rad51 and Rad54 Recombination Proteins*
H. Jiang (1996)
10.1038/30037
Catalysis of homologous DNA pairing by yeast Rad51 and Rad54 proteins
G. Petukhova (1998)
10.1073/PNAS.94.17.9214
Role of Saccharomyces cerevisiae Msh2 and Msh3 repair proteins in double-strand break-induced recombination.
N. Sugawara (1997)
Genetic and physical analysis of double-strand break repair and recombination in Saccharomyces cerevisiae.
N. Rudin (1989)
The subtelomeric Y' repeat family in Saccharomyces cerevisiae: an experimental system for repeated sequence evolution.
E. Louis (1990)
10.1126/SCIENCE.8456314
Similarity of the yeast RAD51 filament to the bacterial RecA filament.
T. Ogawa (1993)
10.1128/MCB.19.12.8083
Telomere-Telomere Recombination Is an Efficient Bypass Pathway for Telomere Maintenance in Saccharomyces cerevisiae
S. Teng (1999)
10.1016/S1097-2765(05)00094-8
Telomerase-independent lengthening of yeast telomeres occurs by an abrupt Rad50p-dependent, Rif-inhibited recombinational process.
S. Teng (2000)
Homologous recombination in Escherichia coli: dependence on substrate length and homology.
P. N. Shen (1986)
10.1101/GAD.11.9.1111
Yeast Rad55 and Rad57 proteins form a heterodimer that functions with replication protein A to promote DNA strand exchange by Rad51 recombinase.
P. Sung (1997)
10.1128/MCB.13.7.3937
Substrate length requirements for efficient mitotic recombination in Saccharomyces cerevisiae.
S. Jinks-Robertson (1993)
10.1093/NAR/29.6.1317
DNA-binding and strand-annealing activities of human Mre11: implications for its roles in DNA double-strand break repair pathways.
M. de Jager (2001)
10.1038/34943
Stimulation by Rad52 of yeast Rad51- mediated recombination
A. Shinohara (1998)
10.1128/MCB.21.19.6559-6573.2001
Intrachromatid Excision of Telomeric DNA as a Mechanism for Telomere Size Control in Saccharomyces cerevisiae
M. Bucholc (2001)
10.1016/S1097-2765(00)00057-5
Rad54 protein is targeted to pairing loci by the Rad51 nucleoprotein filament.
A. Mazin (2000)
10.1074/jbc.274.48.33839
Single Strand DNA Binding and Annealing Activities in the Yeast Recombination Factor Rad59*
G. Petukhova (1999)
10.1101/GAD.13.10.1276
Nbs1 potentiates ATP-driven DNA unwinding and endonuclease cleavage by the Mre11/Rad50 complex.
T. Paull (1999)
10.1101/GAD.10.16.2025
A Rad52 homolog is required for RAD51-independent mitotic recombination in Saccharomyces cerevisiae.
Y. Bai (1996)
10.1128/MCB.21.6.2048-2056.2001
Genetic Requirements for RAD51- andRAD54-Independent Break-Induced Replication Repair of a Chromosomal Double-Strand Break
L. Signon (2001)
10.1128/MCB.18.4.2045
Expansions and Contractions in a Tandem Repeat Induced by Double-Strand Break Repair
F. Pâques (1998)
10.1073/PNAS.92.15.6925
Complex formation in yeast double-strand break repair: participation of Rad51, Rad52, Rad55, and Rad57 proteins.
S. Hays (1995)
10.1128/MCB.21.5.1819-1827.2001
Two Survivor Pathways That Allow Growth in the Absence of Telomerase Are Generated by Distinct Telomere Recombination Events
Q. Chen (2001)
Purification and characterization of the SRS2 DNA helicase of the yeast Saccharomyces cerevisiae.
L. Rong (1993)
10.1073/PNAS.93.20.10729
DNA strand annealing is promoted by the yeast Rad52 protein.
U. Mortensen (1996)
10.1093/emboj/20.4.905
The Saccharomyces cerevisiae WRN homolog Sgs1p participates in telomere maintenance in cells lacking telomerase
F. B. Johnson (2001)
10.1128/MCB.16.5.2164
Cell cycle and genetic requirements of two pathways of nonhomologous end-joining repair of double-strand breaks in Saccharomyces cerevisiae.
J. K. Moore (1996)
10.1073/PNAS.97.26.14500
HO endonuclease-induced recombination in yeast meiosis resembles Spo11-induced events.
A. Malkova (2000)
10.1128/MCB.20.4.1194-1205.2000
RAD51 Is Required for the Repair of Plasmid Double-Stranded DNA Gaps from Either Plasmid or Chromosomal Templates
S. Bärtsch (2000)
10.1128/MMBR.63.2.349-404.1999
Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae
F. Pâques (1999)
10.1016/0092-8674(93)90234-H
An alternative pathway for yeast telomere maintenance rescues est1− senescence
V. Lundblad (1993)
10.1101/GAD.875901
RAD51-independent break-induced replication to repair a broken chromosome depends on a distant enhancer site.
A. Malkova (2001)
Removal of one nonhomologous DNA end during gene conversion by a RAD1- and MSH2-independent pathway.
M. Colaiácovo (1999)
The yeast recombinational repair protein Rad59 interacts with Rad52 and stimulates single-strand annealing.
A. Davis (2001)
10.1038/373084A0
DNA structure-dependent requirements for yeast RAD genes in gene conversion
N. Sugawara (1995)
10.1128/MCB.17.11.6765
Two pathways for removal of nonhomologous DNA ends during double-strand break repair in Saccharomyces cerevisiae.
F. Pâques (1997)
10.1128/MCB.20.14.5300-5309.2000
DNA Length Dependence of the Single-Strand Annealing Pathway and the Role of Saccharomyces cerevisiae RAD59 in Double-Strand Break Repair
N. Sugawara (2000)
10.1093/emboj/16.9.2535
Recombinational repair in yeast: functional interactions between Rad51 and Rad54 proteins
B. Clever (1997)
RDH54, a RAD54 homologue in Saccharomyces cerevisiae, is required for mitotic diploid-specific recombination and repair and for meiosis.
H. Klein (1997)



This paper is referenced by
10.4267/2042/54135
RAD52 (RAD52 homolog (S. cerevisiae))
Bh Lok (2014)
10.4161/cc.4.12.2257
Preferential Occurrence of Chromosome Breakpoints within Early Replicating Regions in Neuroblastoma
I. Janoueix-Lerosey (2005)
10.1016/j.dnarep.2010.02.001
RAD51 loss of function abolishes gene targeting and de-represses illegitimate integration in the moss Physcomitrella patens.
D. G. Schaefer (2010)
10.1016/j.dnarep.2009.12.007
Yeast as a model system to study RecQ helicase function.
Thomas M. Ashton (2010)
10.1128/MCB.25.16.7226-7238.2005
Saccharomyces cerevisiae as a Model System To Define the Chromosomal Instability Phenotype
C. Putnam (2005)
10.1101/2020.02.24.962423
A Rad51-Independent Pathway Promotes Single-Strand Template Repair in Gene Editing
Danielle N Gallagher (2020)
10.1016/j.mrfmmm.2017.06.007
So similar yet so different: The two ends of a double strand break.
K. Kim (2018)
An investigation into the initiation of VSG switching in Trypanosoma brucei
R. Devlin (2015)
10.1038/s41580-019-0152-0
DNA double-strand break repair-pathway choice in somatic mammalian cells
R. Scully (2019)
10.1016/J.MOLCEL.2005.06.001
Crosstalk between SUMO and ubiquitin on PCNA is mediated by recruitment of the helicase Srs2p.
E. Papouli (2005)
10.1534/genetics.106.067447
The Role Of Nonhomologous End-Joining Components in Telomere Metabolism in Kluyveromyces lactis
S. Carter (2007)
10.1016/j.molcel.2014.06.025
Frequent Interchromosomal Template Switches during Gene Conversion in S. cerevisiae.
Olga Tsaponina (2014)
10.1073/pnas.1232239100
Saccharomyces cerevisiae chromatin-assembly factors that act during DNA replication function in the maintenance of genome stability
K. Myung (2003)
Genes required to maintain telomeres in the absence of telomerase in Saccharomyces cerevisiae
Mohammad Kdaimes Alotaibi (2012)
Running Title : Mre 11 in Telomere Recombination Pathways ( telomere , recombination , Mre 11 , telomerase )
I. S. Joseph (2010)
10.1534/genetics.105.055244
The Effects of Mismatch Repair and RAD1 Genes on Interchromosomal Crossover Recombination in Saccharomyces cerevisiae
A. Nicholson (2006)
10.1016/j.molcel.2016.12.003
Homology Requirements and Competition between Gene Conversion and Break-Induced Replication during Double-Strand Break Repair.
A. Mehta (2017)
10.1016/j.dnarep.2008.09.010
Inhibition of DNA double-strand break repair by the Ku heterodimer in mrx mutants of Saccharomyces cerevisiae.
Brian M. Wasko (2009)
A screen for synthetic phenotypes reveals new Sae2 functions and interactions in the repair of DNA double-strand breaks
E. Gobbini (2016)
10.1101/2020.03.17.994905
RAD50 promotes DNA repair by homologous recombination and restrains antigenic variation in African trypanosomes
Ann-Kathrin Mehnert (2020)
10.1080/10409238.2017.1314444
Break induced replication in eukaryotes: mechanisms, functions, and consequences
Cynthia J. Sakofsky (2017)
10.1016/S1097-2765(03)00183-7
Equal sister chromatid exchange is a major mechanism of double-strand break repair in yeast.
S. González-Barrera (2003)
10.1007/s00438-005-1108-y
Regulation of homologous integration in yeast by the DNA repair proteins Ku70 and RecQ
Y. Yamana (2005)
10.1016/S1568-7864(03)00121-6
The Rad52-Rad59 complex interacts with Rad51 and replication protein A.
A. Davis (2003)
Regulation of DNA-end resection at DNA double strand breaks and stalled replication forks
M. Villa (2018)
10.1016/j.febslet.2004.04.058
Alternatives to telomerase: keeping linear chromosomes via telomeric circles
L. Tomáška (2004)
10.1093/abbs/gmw043
Sharpening the ends for repair: mechanisms and regulation of DNA resection.
Sharad C. Paudyal (2016)
10.1371/journal.pgen.1003192
Single-Stranded Annealing Induced by Re-Initiation of Replication Origins Provides a Novel and Efficient Mechanism for Generating Copy Number Expansion via Non-Allelic Homologous Recombination
Kenneth J. Finn (2013)
10.1016/J.BBRC.2005.02.106
Interaction of hRad51 and hRad52 with MCM complex: a cross-talk between recombination and replication proteins.
Ashish Shukla (2005)
10.1093/nar/gkm488
Different genetic requirements for repair of replication-born double-strand breaks by sister-chromatid recombination and break-induced replication
F. Cortés-Ledesma (2007)
10.1534/genetics.108.087189
Mechanisms of Rad52-Independent Spontaneous and UV-Induced Mitotic Recombination in Saccharomyces cerevisiae
Eric Coïc (2008)
10.1016/S0092-8674(03)00886-9
Srs2 and Sgs1–Top3 Suppress Crossovers during Double-Strand Break Repair in Yeast
G. Ira (2003)
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