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Distribution Of Bacterioplankton In Meromictic Lake Saelenvannet, As Determined By Denaturing Gradient Gel Electrophoresis Of PCR-amplified Gene Fragments Coding For 16S RRNA.

Lise Ovreås, Larry Forney, Frida Lise Daae, Vigdis L. Torsvik
Published 1997 · Medicine, Biology
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The community structure of bacterioplankton in meromictic Lake Saelenvannet was examined by PCR amplification of the V3 region of 16S rRNA from microbial communities recovered from various depths in the water column. Two different primer sets were used, one for amplification of DNA from the domain Bacteria and another specific for DNA from the domain Archaea. Amplified DNA fragments were resolved by denaturing gradient gel electrophoresis (DGGE), and the resulting profiles were reproducible and specific for the communities from different depths. Bacterial diversity estimated from the number and intensity of specific fragments in DGGE profiles decreased with depth. The reverse was true for the Archaea, with the diversity increasing with depth. Hybridization of DGGE profiles with oligonucleotide probes specific for phylogenetic groups of microorganisms showed the presence of both sulfate-reducing bacteria and methanogens throughout the water column, but they appeared to be most abundant below the chemocline. Several dominant fragments in the DGGE profiles were excised and sequenced. Among the dominant populations were representatives related to Chlorobium phaeovibrioides, chloroplasts from eukaryotic algae, and unidentified Archaea.
This paper references
16S rRNA sequences reveal numerous uncultured microorganisms in a natural community
David M. Ward (1990)
Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16s rRNA
G. Muyzer (1993)
Phylogenetic diversity of subsurface marine microbial communities from the Atlantic and Pacific oceans.
Jed A. Fuhrman (1993)
Molecular methods to study the organization of microbial communities
Gerard Muyzer (1995)
A simple and rapid electrophoresis method to detect sequence variation in PCR-amplified DNA fragments.
Cathrin Wawer (1995)
Development and application of nucleic acid probes, p. 205–248
D. A. Stahl (1991)
Blooms of sequence-specific culturable bacteria in the sea
A S Rehnstam (1993)
Genetic diversity in Sargasso Sea bacterioplankton
S. Giovannoni (1990)
Archaea in coastal marine environments.
Edward F. DeLong (1992)
Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR.
Marcelino T Suzuki (1996)
Molecular and microscopic identification of sulfate-reducing bacteria in multispecies biofilms.
Rudolf I. Amann (1992)
PCR-based preparation of 23S rRNA-targeted group-specific polynucleotide probes.
Wolfgang Ludwig (1994)
Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community.
Mike Ferris (1996)
The methanogenic bacteria, p. 719–767
W. B. Whitman (1992)
Distribution of bacterial populations in a stratified fjord (mariager fjord, denmark) quantified by in situ hybridization and related to chemical gradients in the water column.
Niels B. Ramsing (1996)
Diversity of uncultured microorganisms associated with the seagrass Halophila stipulacea estimated by restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA genes.
Stefan Weidner (1996)
Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations.
Rudolf I. Amann (1990)
Identification of bacteria in a biodegraded wall painting by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA.
Sabine Rölleke (1996)
Taxonomic diversity and metabolic activity of microbial communities in the water column of the central Baltic Sea
Manfred G. Höfle (1995)
Use of ribosomal RNA-based molecular probes for characterization of complex microbial communities in anaerobic biofilms
Lutgarde Raskin (1995)
Basic local alignment search tool.
Stephen F. Altschul (1990)
Bacterial population dynamics in a meromictic lake.
Pam Tuomi (1997)
General microbiology
H. G. Schlegel (1986)
Distribution of sulfate-reducing bacteria in a stratified fjord (Mariager Fjord, Denmark) as evaluated by most-probable-number counts and denaturing gradient gel electrophoresis of PCR-amplified ribosomal DNA fragments.
Andreas P. Teske (1996)
Oligonucleotide probes to 16S rRNA of methanogenic bacteria, abstr
T. Torsvik (1993)
Detection and localization of single base changes by denaturing gradient gel electrophoresis.
R. Myers (1987)
Methods of Seawater Analysis
Klaus Grasshoff (1976)
Microbial activities in a permanently stratified estuary. I. Primary production and sulfate reduction
G. Indrebø (1979)
16S/23S rRNA sequencing
David J. W. Lane (1991)
Microbial activities in a permanently stratified estuary. II. Microbial activities at the oxic-anoxic interface
G. Indrebø (1979)
Differential amplification of rRNA genes by polymerase chain reaction.
A L Reysenbach (1992)
Biology of Microorganisms
Thomas D. Brock (1970)
Oligonucle - otide probes to 16 S rRNA of methanogenic bacteria , abstr . N19 , p . 300
V. Torsvik (1993)
Phylogenetic compositions of bacterioplankton from two California estuaries compared by denaturing gradient gel electrophoresis of 16S rDNA fragments.
A. Murray (1996)
The use of DAPI for identifying and counting aquatic microflora1
Karen Glaus Porter (1980)
Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells.
Edward F. DeLong (1989)
High abundance of Archaea in Antarctic marine picoplankton
Edward F. DeLong (1994)
Novel major archaebacterial group from marine plankton
Alison A. Davis (1992)
Potential risks of gene amplification by PCR as determined by 16S rDNA analysis of a mixed-culture of strict barophilic bacteria
W. Liesack (2006)

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