Online citations, reference lists, and bibliographies.
← Back to Search

Reexamination Of The Association Between Melting Point, Buoyant Density, And Chemical Base Composition Of Deoxyribonucleic Acid.

J. De Ley
Published 1970 · Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
The equations currently used for the calculation of the chemical base composition of deoxyribonucleic acid (DNA), expressed as moles per cent guanine plus cytosine (% GC), from either buoyant density (rho) or midpoint of thermal denaturation (T(m)) were recalculated by using only sets of data on DNA determined with the same strains. All available information from the literature was screened and supplemented by unpublished data. The results were calculated by regression and correlation analysis and treated statistically. From the data on 96 strains of bacteria, it was calculated that% GC = 2.44 (T(m) - 69.4). T(m) appears to be unaffected by the substitution of cytosine by hydroxymethylcytosine. This equation is also valid for nonbacterial DNA. From the data on 84 strains of bacteria, the relation% GC = 1038.47 (-1.6616) was calculated. The constants in this equation are slightly modified when data on nonbacterial DNA are included. Both correlations differ only slightly from those currently used, but now they lean on a statistically sound basis. As a control, the relation between rho and T(m) was calculated from data of 197 strains; it agrees excellently with the above two equations.



This paper is referenced by
10.1007/BF01318064
Biophysical properties of virions of human adenovirus of the type 6 and its DNA
T. Tikchonenko (2005)
10.1007/BF02010552
DNA base compositions of halophilic and nonhalophilicBacillus firmus strains of marine origin
Hans -Jürgen Rüger (2005)
10.1128/AEM.54.2.561-565.1988
Fermentative degradation of nonionic surfactants and polyethylene glycol by enrichment cultures and by pure cultures of homoacetogenic and propionate-forming bacteria.
S. Wagener (1988)
Sulfolobus hakonensis sp. nov., a Novel Acidothermophilic Archaeon Species of
S. Takayanagi (1996)
10.1099/00207713-49-4-1559
Pseudomonas gessardii sp. nov. and Pseudomonas migulae sp. nov., two new species isolated from natural mineral waters.
S. Verhille (1999)
10.1099/IJS.0.02031-0
Characterization of rhizobia isolated from legume species within the genera Astragalus and Lespedeza grown in the Loess Plateau of China and description of Rhizobium loessense sp. nov.
G. Wei (2003)
10.1099/00207713-47-3-846
Pseudomonas monteilii sp. nov., isolated from clinical specimens.
M. Elomari (1997)
10.1099/00221287-132-4-939
Differential scanning calorimetry of bacteria.
C. Miles (1986)
Numerical Analysis and DNA Base Compositions of Some Ther ' mophilic Bacillus Species ANGEL 0
Zu (2008)
Characterization and Comparison of the D N A s of the Three Closely Related Bacteriophages gd , ge and gf with the Genome D N A of the Hydrogen-oxidizing Host Strain Pseudomonas pseudoflava GA 3
(2007)
10.1099/ijs.0.65632-0
Rhizobium oryzae sp. nov., isolated from the wild rice Oryza alta.
G. Peng (2008)
10.1099/00207713-38-1-63
Taxonomic study of Bacillus coagulans Hammer 1915 with a proposal for Bacillus smithii sp. nov
L. K. Nakamura (1988)
10.1099/00207713-31-3-236
Confirmation of Bacteroides gingivalis as a Species Distinct from Bacteroides asaccharolyticus
T. V. Steenbergen (1981)
10.1099/00207713-47-4-1165
Aeromonas popoffii sp. nov., a mesophilic bacterium isolated from drinking water production plants and reservoirs.
G. Huys (1997)
10.1007/978-94-009-0889-5
Nitrogen Fixation with Non-Legumes
F. Skinner (1989)
10.1128/AEM.56.3.782-787.1990
High diversity in DNA of soil bacteria.
V. Torsvik (1990)
10.1099/00207713-37-4-422
Transfer of Xanthomonas ampelina Panagopoulos 1969 to a New Genus, Xylophilus gen. nov., as Xylophilus ampelinus (Panagopoulos 1969) comb. nov.
A. Willems (1987)
10.1099/00207713-36-3-435
Isolation and Characterization of Acidothermus cellulolyticus gen. nov., sp. nov., a New Genus of Thermophilic, Acidophilic, Cellulolytic Bacteria
A. Mohagheghi (1986)
Rhizobium huakuii sp . nov . Isolated from the Root Nodules of As t raga 1 us sin ic u s
W. ()
10.1007/BF00399454
Hydrogen-dependent organisms from the human gingival crevice resembling Vibrio succinogenes
W. H. Palenstein Helderman (2004)
FUNCTIONAL IMPACT OF GENETICALLY MODIFIED MICRO-ORGANISMS ON THE SOIL ECOSYSTEM
J. Schepers (2008)
10.1099/00207713-48-2-481
Vibrio pectenicida sp. nov., a pathogen of scallop (Pecten maximus) larvae.
C. Lambert (1998)
10.1111/lam.12790
Isolation and characterization of a novel deoxynivalenol‐transforming strain Paradevosia shaoguanensis DDB001 from wheat field soil
Y. Wang (2017)
10.1099/00207713-41-1-88
Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov.
P. Vandamme (1991)
10.1099/ijs.0.021543-0
Hansschlegelia zhihuaiae sp. nov., isolated from a polluted farmland soil.
Y. Wen (2011)
10.1099/ijs.0.065888-0
Chryseobacterium takakiae sp. nov., a member of the phylum Bacteroidetes isolated from Takakia lepidozioides.
R. Zhao (2015)
10.1099/ijs.0.000353
Diaphorobacter polyhydroxybutyrativorans sp. nov., a novel poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-degrading bacterium isolated from biofilms.
Tianlei Qiu (2015)
10.1099/ijs.0.028936-0
Bacillus endoradicis sp. nov., an endophytic bacterium isolated from soybean root.
Y. Zhang (2012)
Genetic, morphological and physiological relationships among coryneform bacteria
W. Crombach (1974)
10.1007/BF00403233
Characterization of a new platelet-forming purple sulfur bacterium, Amoebobacter pedioformis sp. nov.
B. Eichler (2004)
10.1099/00221287-129-4-1159
Microbial Ecology of Volcanic Sulphidogenesis: Isolation and Characterization of Thermodesulfobacterium commune gen. nov. and sp. nov.,
J. Zeikus (1983)
10.1099/00207713-46-4-926
Reclassification of Flavobacterium odoratum (Stutzer 1929) strains to a new genus, Myroides, as Myroides odoratus comb nov and Myroides odoratimimus sp nov.
M. Vancanneyt (1996)
See more
Semantic Scholar Logo Some data provided by SemanticScholar