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Hydrogen Peroxide Produced Inside Mitochondria Takes Part In Cell-to-cell Transmission Of Apoptotic Signal

O. Pletjushkina, E. Fetisova, K. Lyamzaev, O. Ivanova, L. V. Domnina, M. Vyssokikh, A. Pustovidko, A. V. Alexeevski, D. A. Alexeevski, J. Vasiliev, M. Murphy, B. V. Chernyak, V. Skulachev
Published 2006 · Biology, Medicine

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In monolayer of HeLa cells treated with tumor necrosis factor (TNF), apoptotic cells formed clusters indicating possible transmission of apoptotic signal via the culture media. To investigate this phenomenon, a simple method of enabling two cell cultures to interact has been employed. Two coverslips were placed side by side in a Petri dish, one coverslip covered with apoptogen-treated cells (the inducer) and another with non-treated cells (the recipient). TNF, staurosporine, or H2O2 treatment of the inducer cells is shown to initiate apoptosis on the recipient coverslip. This effect is increased by a catalase inhibitor aminotriazole and is arrested by addition of catalase or by pre-treatment of either the inducer or the recipient cells with nanomolar concentrations of mitochondria-targeted cationic antioxidant MitoQ (10-(6′-ubiquinolyl)decyltriphenylphosphonium), which specifically arrests H2O2-induced apoptosis. The action of MitoQ is abolished by an uncoupler preventing accumulation of MitoQ in mitochondria. It is concluded that reactive oxygen species (ROS) produced by mitochondria in the apoptotic cells initiate the release of H2O2 from these cells. The H2O2 released is employed as a long-distance cell suicide messenger. In processing of such a signal by the recipient cells, mitochondrial ROS production is also involved. It is suggested that the described phenomenon may be involved in expansion of the apoptotic region around a damaged part of the tissue during heart attack or stroke as well as in “organoptosis”, i.e. disappearance of organs during ontogenesis.
This paper references
Mechanism of Coupling of Oxidative Phosphorylation and the Membrane Potential of Mitochondria
E. Liberman (1969)
Conversion of biomembrane-produced energy into electric form. I. Submitochondrial particles.
L. Grinius (1970)
Conversion of biomembrane-produced energy into electric form. IV. General discussion.
E. Liberman (1970)
Energetics of Biological Membranes [in Russian], Nauka, Moscow
V. P. Skulachev (1989)
Viruses hold the keys of death.
Wyllie Ah (1996)
Role of uncoupled and non-coupled oxidations in maintenance of safely low levels of oxygen and its one-electron reductants.
V. Skulachev (1996)
Membrane-Linked Systems Preventing Superoxide Formation
V. Skulachev (1997)
Enhancement of the herpes simplex virus thymidine kinase/ganciclovir bystander effect and its antitumor efficacy in vivo by pharmacologic manipulation of gap junctions.
R. Touraine (1998)
Possible role of reactive oxygen species in antiviral defense.
V. Skulachev (1998)
Thyroxine enhancement and the role of reactive oxygen species in tadpole tail apoptosis.
A. Kashiwagi (1999)
Mitochondrial physiology and pathology; concepts of programmed death of organelles, cells and organisms.
V. Skulachev (1999)
Production of a signal by irradiated cells which leads to a response in unirradiated cells characteristic of initiation of apoptosis
F. Lyng (2000)
Reactive Oxygen Species (Ros-Induced) Ros Release: A New Phenomenon Accompanying Induction of the Mitochondrial Permeability Transition in Cardiac Myocytes
D. Zorov (2000)
Clustering of apoptotic cells via bystander killing by peroxides
K. Reznikov (2000)
Inhibition of angiogenesis and induction of apoptosis are involved in E1A-mediated bystander effect and tumor suppression.
R. Shao (2000)
Reactive oxygen and nitrogen species: efficient, selective, and interactive signals during intercellular induction of apoptosis.
G. Bauer (2000)
Direct evidence for the participation of gap junction-mediated intercellular communication in the transmission of damage signals from alpha -particle irradiated to nonirradiated cells.
E. Azzam (2001)
Programmed cell death, mitochondria and the plant hypersensitive response
E. Lam (2001)
Development of lipophilic cations as therapies for disorders due to mitochondrial dysfunction
M. Murphy (2001)
Oligomycin, inhibitor of the F0 part of H+-ATP-synthase, suppresses the TNF-induced apoptosis
L. A. Shchepina (2002)
The apoplastic oxidative burst in response to biotic stress in plants: a three-component system.
G. Bolwell (2002)
Bystander effect produced by radiolabeled tumor cells in vivo
L. Xue (2002)
Prevention of Mitochondrial Oxidative Damage Using Targeted Antioxidants
G. F. Kelso (2002)
Free radicals in the physiological control of cell function.
W. Dröge (2002)
Low-dose radiation effects: experimental hematology and the changing paradigm.
C. Mothersill (2003)
Topics in Current Genetics. Model Systems in Ageing (Nystrom
V. P. Skulachev (2003)
Characterization of Superoxide-producing Sites in Isolated Brain Mitochondria*
A. Kudin (2004)
Hydrogen peroxide is a diffusible paracrine signal for the induction of epithelial cell death by activated myofibroblasts
Meghna Waghray (2005)

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A. Bhattacharjee (2016)
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I. Zakharova (2016)
In vitro cell-based assays for evaluation of antioxidant potential of plant-derived products
L. C. Nascimento da Silva (2016)
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S. Claro (2014)
Apoptosis and necrosis induced by ultraviolet radiation in the presence of autologous plasma
V. Artyukhov (2014)
New data on programmed aging — slow phenoptosis
M. Skulachev (2014)
Cytochrome C as an amplifier of ROS release in mitochondria.
O. Akopova (2012)
Human leucocyte antigen class Ib molecules in pregnancy success and early pregnancy loss.
M. Dahl (2012)
Evaluation of Hydrogen Peroxide Induced Cardiotoxicity in Sixth Day Chick Embryo
Poonam Musale (2012)
Involvement of ROS in the inhibitory effect of thermotherapy combined with chemotherapy on A549 human lung adenocarcinoma cell growth through the Akt pathway.
L. Wang (2012)
Models of Mitochondrial Oxidative Stress
E. Cadenas (2011)
Age-Associated Alterations in the F344XBN Rat Heart and the Efficacy of Chronic Acetaminophen Ingestion as a Therapeutic Approach
Sunil K. Kakarla (2011)
DNA fragmentation of human lymphocytes in dynamics of development of apoptosis induced by action of UV radiation and reactive oxygen species
V. Artyukhov (2011)
Metabolic stress-induced programmed cell death in Xanthomonas.
S. Wadhawan (2010)
Chronic acetaminophen attenuates age-associated increases in cardiac ROS and apoptosis in the Fischer Brown Norway rat
Sunil K. Kakarla (2010)
Mechanisms of interaction of non-thermal plasma with living cells
S. Kalghatgi (2010)
Premature aging syndrome in ataxia telangiectasia patients
E. A. Polubotko (2009)
Molecular mechanisms of transformation of SkQ mitotropic quinones and the search for new approaches to creation of selective free radical traps
S. A. Eremeyev (2009)
The mitochondrial kinase PINK1, stress response and Parkinson’s disease
M. Jendrach (2009)
Dopamine alleviation of diaphragm contractile dysfunction and reduction of deoxyribonucleic acid damage in rats.
J. Pierce (2008)
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