Online citations, reference lists, and bibliographies.
← Back to Search

The Efficacy Of The Heat Killing Of Mycobacterium Tuberculosis

C. Doig, A. L. Seagar, B. Watt, K. Forbes
Published 2002 · Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
There is concern that current procedures for the heat inactivation of Mycobacterium tuberculosis may not be adequate. This raises serious safety issues for laboratory staff performing molecular investigations such as IS6110 restriction fragment length polymorphism typing. This paper confirms that the protocol of van Embden et al, as performed routinely in this laboratory, is safe and effective for the heat inactivation of M tuberculosis. This procedure involves complete immersion of a tube containing a suspension of one loopfull of growth in a water bath at 80°C for 20 minutes. Seventy four isolates were included in this investigation. Despite prolonged incubation for 20 weeks, none of the heat killed M tuberculosis suspensions produced visible colonies or gave a positive growth signal from liquid culture. This method did not affect the integrity of the DNA for subsequent molecular investigations.
This paper references



This paper is referenced by
10.9734/BJMMR/2015/16945
Evaluation of simple and cost-effective DNA preparation and subsequent PCR amplification for clinically relevant mycobacteria.
Verónica Zaragoza Gómez (2015)
Novel particulate BCG-loaded delivery system for mucosal immunization against tuberculosis
L. A. Caetano (2016)
10.1371/journal.pone.0232632
Simultaneous detection of Mycobacterium tuberculosis complex and resistance to Rifampicin and Isoniazid by MDR/MTB ELITe MGB® Kit for the diagnosis of tuberculosis
Francesco Bisognin (2020)
10.5588/ijtld.17.0139
Accuracy of giant African pouched rats for diagnosing tuberculosis: comparison with culture and Xpert® MTB/RIF.
C. Mulder (2017)
10.1128/JCM.43.6.2996-2997.2005
Extraction of Mycobacterium tuberculosis DNA: a Question of Containment
W. Somerville (2005)
10.25392/LEICESTER.DATA.11799795.V1
Use of face-mask sampling as a means of characterising the microbiota exhaled from human respiratory tract in health and disease
M. Abdulwhhab (2020)
10.1016/J.TUBE.2007.05.014
Deletion-targeted multiplex PCR (DTM-PCR) for identification of Beijing/W genotypes of Mycobacterium tuberculosis.
J. Chen (2007)
10.3390/md14050090
Effect of Experimental Parameters on Alginate/Chitosan Microparticles for BCG Encapsulation
Liliana Aranha Caetano (2016)
10.1371/journal.pone.0001100
Solar Disinfection of MODS Mycobacterial Cultures in Resource-Poor Settings
R. Nathavitharana (2007)
10.1128/JCM.01778-18
Heat Inactivation Renders Sputum Safe and Preserves Mycobacterium tuberculosis RNA for Downstream Molecular Tests
W. Sabiiti (2019)
10.1155/2012/768603
Tuberculosis Diagnostics in the New Millennium: Role in TB Identification and Control
S. Chakravorty (2012)
10.1016/j.mimet.2015.07.010
Laboratory evaluation of a specimen transport medium for downstream molecular processing of sputum samples to detect Mycobacterium tuberculosis.
S. Omar (2015)
Diagnostic Accuracy of IS 6110 Insertion Gene , Hsp 65 , and Xpert MTB / RIF for Rapid Diagnosis of Pulmonary Tuberculosis
Aymen Awad Abdelhaleem (2017)
10.1016/j.mimet.2017.09.013
Thermal lysis and isothermal amplification of Mycobacterium tuberculosis H37Rv in one tube.
Prasad Shetty (2017)
10.1016/j.jhin.2011.12.018
High level disinfection of a home care device; to boil or not to boil?
Kevin L. Winthrop (2012)
10.1186/s13104-017-2522-z
Dry-heat inactivation of “Mycobacterium canettii”
Djaltou Aboubaker Osman (2017)
Study of Electrokinetic Response for Drug Susceptibility of Mycobacterium Tuberculosis Complex Cells
S. Inoue (2014)
10.1186/1471-2334-5-4
Viability testing of material derived from Mycobacterium tuberculosis prior to removal from a Containment Level-III Laboratory as part of a Laboratory Risk Assessment Program
K. Blackwood (2005)
10.4314/THRB.V14I2.5
APOPO's tuberculosis research agenda: achievements, challenges and prospects.
Negussie W Beyene (2012)
Tittle : Heat-inactivation renders sputum safe and preserves Mycobacterium 1 tuberculosis RNA for downstream molecular tests 2 Running tittle : Heat-inactivation of sputum preserves RNA 3
Wilber Sabiiti (2019)
10.1101/2020.04.05.026864
DNA Thermo-Protection Facilitates Whole Genome Sequencing of Mycobacteria Direct from Clinical Samples by the Nanopore Platform
Sophie George (2020)
10.1093/jac/dkn259
The effect of oxidative stress on the mutation rate of Mycobacterium tuberculosis with impaired catalase/peroxidase function.
D. M. O'Sullivan (2008)
10.1101/726430
In-host population dynamics of M. tuberculosis during treatment failure
R. Vargas (2019)
10.1155/2019/1629258
Blockade Effects of Anti-Interferon- (IFN-) γ Autoantibodies on IFN-γ-Regulated Antimicrobial Immunity
D. Krisnawati (2019)
10.1007/s00103-016-2451-3
Mycobacterium leprae – der Erreger von Lepra
(2016)
10.1002/jcla.22716
Direct detection from clinical sputum samples to differentiate live and dead Mycobacterium Tuberculosis
Jie Lu (2019)
10.1128/JCM.44.1.254-256.2006
Safe Mycobacterium tuberculosis DNA Extraction Method That Does Not Compromise Integrity
R. Warren (2006)
10.1371/journal.pone.0024720
Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Identification of Mycobacteria in Routine Clinical Practice
A. El Khéchine (2011)
10.1155/2012/716989
Pouched Rats' Detection of Tuberculosis in Human Sputum: Comparison to Culturing and Polymerase Chain Reaction
A. Mahoney (2012)
Innovative approaches to tuberculosis diagnosis with emphasis on nucleic acid amplification tests in a resource constrained high burden tuberculosis setting
S. Omar (2015)
10.1016/j.meegid.2014.01.031
rs2243268 and rs2243274 of Interleukin-4 (IL-4) gene are associated with reduced risk for extrapulmonary and severe tuberculosis in Chinese Han children.
H. Qi (2014)
Best Practices for Identification of Mycobacterium S pecies U sing Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
(2019)
See more
Semantic Scholar Logo Some data provided by SemanticScholar