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The Green Fluorescent Protein.

R. Tsien
Published 1998 · Biology, Medicine

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In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins in biochemistry and cell biology. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. High-resolution crystal structures of GFP offer unprecedented opportunities to understand and manipulate the relation between protein structure and spectroscopic function. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms. Mutagenesis and engineering of GFP into chimeric proteins are opening new vistas in physiological indicators, biosensors, and photochemical memories.
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Onset timing of transient gene expression depends on cell division.
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Characterization of the CAG 140 Mouse Model of Huntington's Disease and the Effects of rAAV Delivered shRNA and hGFP
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The use of aequorins to record and visualize Ca(2+) dynamics: from subcellular microdomains to whole organisms.
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Modulation of protein properties in living cells using nanobodies
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Identification of the amino acid residues responsible for the reversible photoconversion of the monomeric red fluorescent protein TagRFP
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