← Back to Search
Regulation Of Lipoprotein Lipase Secretion In Murine Macrophages During Foam Cell Formation In Vitro. Effect Of Triglyceride-rich Lipoproteins.
O. Sofer, M. Fainaru, Z. Schafer, R. Goldman
Published 1992 · Biology, Medicine
Download PDFAnalyze on Scholarcy
Triglyceride rich-lipoproteins induce triglyceride accumulation in macrophages, leading to foam cell formation. The correlation between cell triglyceride accumulation and lipoprotein lipase (LPL) secretion in murine macrophages and the role that LPL plays in the accumulation process were examined. LPL secretion is defined as the extracellular LPL activity that accumulates during a 4-hour incubation of treated and untreated cells in a bovine serum albumin-containing RPMI-1640 medium. LPL secretion was suppressed (up to 70%) in a dose- and time-dependent manner when J774.1 cells were incubated with chylomicrons, very low density lipoproteins, and intermediate density lipoproteins but not with low or high density lipoproteins from normolipidemic and hypertriglyceridemic subjects. Oleic acid both suppressed LPL secretion and invoked triglyceride accumulation. Suppression of LPL secretion preceded gross triglyceride accumulation, was reversible, and was not the result of a reduction in LPL mRNA. P388D1 cells neither secreted LPL nor accumulated triglyceride. Inhibition of LPL secretion by tunicamycin in both peritoneal macrophages and J774.1 cells prevented a hypertriglyceridemic very low density lipoprotein-induced triglyceride accumulation, an effect that was counteracted by addition of exogenous LPL. The results suggest that 1) extracellular hydrolysis of lipoprotein triglyceride is a major factor in inducing foam cell formation and 2) LPL secretion may be regulated by cell energy needs, and when these needs are exceeded, LPL secretion is suppressed.
This paper references
Lipoprotein lipase of cultured mesenchymal rat heart cells. IV. Modulation of enzyme activity by VLDL added to the culture medium.
G. Friedman (1979)
The nucleotide sequence of the rat cytoplasmic beta-actin gene.
U. Nudel (1983)
Dependence on Ca2+ of Lipoprotein Lipase Stability
R. Goldman (1990)
Regulation of lipoprotein lipase activity and mRNA content in rat epididymal adipose tissue in vitro by recombinant tumour necrosis factor.
A. Mackay (1990)
Protein measurement with the Folin phenol reagent.
O. H. Lowry (1951)
The sequence of cDNA encoding lipoprotein lipase. A member of a lipase gene family.
T. Kirchgessner (1987)
Cachectin/tumor necrosis factor decreases human adipose tissue lipoprotein lipase mRNA levels, synthesis, and activity.
S. Fried (1989)
Control of lipoprotein lipase secretion in mouse macrophages.
R. Goldman (1989)
Bacterial lipopolysaccharide suppresses the expression of lipoprotein lipase in murine macrophages: a process independent of tumor necrosis factor or interleukin 1.
O. Sopher (1987)
Regulation of lipoprotein lipase.
T. Olivecrona (1993)
c‐fos transfection of 3LL tumor cells turns on MHC gene expression and consequently reduces their metastatic competence
G. Kushtai (1990)
Control of Lipoprotein Lipase Secretion by Macrophages: Effect of Macrophage Differentiation Agents
R. Goldman (1990)
Identification of macrophage-like characteristics in a cultured murine tumor line.
H. Koren (1975)
The response of lipoprotein lipase to feeding and fasting. Evidence for posttranslational regulation.
M. Doolittle (1990)
Regulation of macrophage lipoprotein lipase secretion by the scavenger receptor.
Y. Murata (1988)
Kinetics of product inhibition and mechanisms of lipoprotein lipase activation by apolipoprotein C-II.
I. Posner (1987)
Lipoprotein lipase. Mechanism of product inhibition.
G. Bengtsson (1980)
Plasma lipoprotein conversions.
S. Eisenberg (1986)
Reticulum cell sarcoma: an effector cell in antibody-dependent cell-mediated immunity.
P. Ralph (1975)
Secretion of lipoprotein lipase by macrophages in culture.
J. C. Khoo (1981)
MP: Regulation of lipoprotein lipase activity and mRNA content in rat epididymal adipose tissue in vitro by recombinant tumor necrosis factor
AG MacKay (1990)
Macrophage lipoprotein receptors
A. Fogelman (1988)
Enhanced lipoprotein lipase secretion from human monocyte-derived macrophages caused by hypertriglyceridemic very low density lipoproteins.
S. Ishibashi (1989)
Regional Accumulations of T Cells, Macrophages, and Smooth Muscle Cells in the Human Atherosclerotic Plaque
L. Jonasson (1986)
Stimulation of Cholesterol Esterification in Rhesus Monkey Arterial Smooth Muscle Cells
R. W. (1977)
Uptake and Degradation of Human Chylomicrons by Macrophages in Culture: Role of Lipoprotein Lipase
A. M. Ostlund-Lindqvist (1983)
Insulin increases the synthetic rate and messenger RNA level of lipoprotein lipase in isolated rat adipocytes.
J. Ong (1988)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
Intracellular regulation of lipoprotein lipase in human monocyte-derived macrophages.
N. Stray (1990)
High-cholesterol diet-induced lipoproteins stimulate lipoprotein lipase secretion in cultured rat alveolar macrophages.
N. Mori (1987)
Hypertriglyceridemic very low density lipoproteins induce triglyceride synthesis and accumulation in mouse peritoneal macrophages.
S. Gianturco (1982)
Effect of feeding and obesity on lipoprotein lipase activity, immunoreactive protein, and messenger RNA levels in human adipose tissue.
J. Ong (1989)
Effects of cytokines on the production of lipoprotein lipase in cultured human macrophages.
U. Querfeld (1990)
Rates of utilization and fates of glucose, glutamine, pyruvate, fatty acids and ketone bodies by mouse macrophages.
P. Newsholme (1987)
Fatty acid control of lipoprotein lipase: a link between energy metabolism and lipid transport.
J. Peterson (1990)
Apolipoprotein E and lipoprotein lipase secreted from human monocyte-derived macrophages modulate very low density lipoprotein uptake.
S. Ishibashi (1990)
Very Low Density Lipoproteins Promote Triglyceride Accumulation in Macrophages
S. R. Bates (1984)
Regulation of the secretion of lipoprotein lipase by mouse macrophages.
S. Behr (1986)
The role of lipoprotein lipase in the metabolism of triglyceride-rich lipoproteins by macrophages.
P. Lindqvist (1983)
Release of endothelial cell lipoprotein lipase by plasma lipoproteins and free fatty acids.
U. Saxena (1989)
Transcriptional activation of the lipoprotein lipase gene in macrophages by dexamethasone.
W. S. Domin (1991)
The beta-VLDL receptor pathway of murine P388D1 macrophages.
S. Gianturco (1986)
Lipoprotein lipase in mouse peritoneal macrophages: the effects of insulin and dexamethasone.
M. Kawakami (1986)
Regulation of lipoprotein lipase secretion by mouse peritoneal macrophages.
D. I. Goldberg (1987)
Recombinant human cachectin/tumor necrosis factor but not interleukin-1 alpha downregulates lipoprotein lipase gene expression at the transcriptional level in mouse 3T3-L1 adipocytes.
R. Zechner (1988)
A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of proteindye binding
MM Bradford (1976)
A stable, radioactive substrate emulsion for assay of lipoprotein lipase.
P. Nilsson-ehle (1976)
The role of glucose and glycosylation in the regulation of lipoprotein lipase synthesis and secretion in rat adipocytes.
J. Ong (1989)
Interaction of lipoprotein lipase with glycosaminoglycans and apolipoprotein C-II: effects of free-fatty-acids.
U. Saxena (1990)
Stimulation of rat heart lipoprotein lipase activity by 4‐aminopyrazolo[3,4‐d]pyrimidine‐induced reduction of plasma triacylglycerol
G. Friedman (1979)
Quantitative determination of serum triglycerides by the use of enzymes.
G. Bucolo (1973)
Enzymatic determination of total serum cholesterol.
C. Allain (1974)
Lipoproteins and atherogenesis. Current concepts.
D. Steinberg (1990)
This paper is referenced by
Effect of dietary cholesterol on postprandial lipoproteins in three phenotypic groups.
P. Clifton (1996)
Oxidized VLDL induces less triglyceride accumulation in J774 macrophages than native VLDL due to an impaired extracellular lipolysis.
M. Jong (2000)
Lipoprotein lipase correlates positively and hepatic lipase inversely with calcific atherosclerosis in homozygous familial hypercholesterolemia.
K. Dugi (1997)
Role of Macrophage-Derived Lipoprotein Lipase in Lipoprotein Metabolism and Atherosclerosis
M. van Eck (2000)
Utilisation of Fatty Acid and Triacylglycerol by Rat Macrophages: the Effect of Endotoxin
D. Hauton (2002)
The role of virgin olive oil components in the modulation of endothelial function.
J. Perona (2006)
Upregulation of TNF-α by Triglycerides is Mediated by MEK1 Activation in Jurkat T Cells
Jaewon Lim (2018)
Implications of endogenous and exogenous lipoprotein lipase for the selective uptake of HDL3-associated cholesteryl esters by mouse peritoneal macrophages.
U. Panzenboeck (1997)
Peroxisome proliferator‐activated receptor (PPAR) agonists decrease lipoprotein lipase secretion and glycated LDL uptake by human macrophages
F. Gbaguidi (2002)
Diabetic state induces lipid loading and altered expression and secretion of lipoprotein lipase in human monocyte-derived macrophages.
A. Dobrian (2000)
Regulation of serum-induced lipid accumulation in human monocyte-derived macrophages by interferon-gamma. Correlations with apolipoprotein E production, lipoprotein lipase activity and LDL receptor-related protein expression.
B. Garner (1997)
Triglyceride (TG) down-regulates expression of MCP-1 and CCR2 in PMA-derived THP-1 macrophages
Y. Kim (2013)
High-Energy Diets, Fatty Acids and Endothelial Cell Function: Implications for Atherosclerosis
B. Hennig (2001)
An investigation of the atherogenicity of intermediate density lipoprotein in people with and without type 2 diabetes mellitus
Ching-Loong Cheung (2009)
Interaction of exercise and fish oil on postprandial lipemia
Y. Liu (2006)
Lysosomal cholesterol accumulation: driver on the road to inflammation during atherosclerosis and non‐alcoholic steatohepatitis
T. Hendrikx (2014)
Increased expression of interleukin-1β in triglyceride-induced macrophage cell death is mediated by p38 MAP kinase.
H. Sung (2012)
Physiological regulation of lipoprotein lipase.
S. Kersten (2014)
Inhibition of Long-Chain Acyl Coenzyme A Synthetases During Fatty Acid Loading Induces Lipotoxicity in Macrophages
V. Saraswathi (2009)
Oxysterols present in atherosclerotic tissue decrease the expression of lipoprotein lipase messenger RNA in human monocyte-derived macrophages.
L. Hultén (1996)
Caspase-2 mediates triglyceride (TG)-induced macrophage cell death
Jaewon Lim (2017)