Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Synthesis, Analytical Characterization, And Osteoblast Adhesion Properties On RGD-grafted Polypyrrole Coatings On Titanium Substrates

E. De Giglio, L. Sabbatini, S. Colucci, G. Zambonin
Published 2000 · Materials Science, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
The covalent attachment of an Arg-Gly-Asp (RGD) containing peptide to polypyrrole(PPy)-coated titanium substrates has been investigated in order to develop a bioactive material of potential use in orthopedic fields. Polypyrrole has been employed as the coating polymer because of its suitability to be electrochemically grown directly onto metallic substrates of different shapes, leading to remarkably adherent films. The synthetic peptide Cys-Gly-(Arg-Gly-Asp)-Ser-Pro-Lys, containing the cell-adhesive region of fibronectin(RGD), has been grafted to the polymer substrate via the cysteine residue using a procedure recently developed in the authors laboratory. The effectiveness of grafting was monitored by X-ray photoelectron spectroscopy (XPS), which assessed the presence of the peptide grafted onto the polymer surface exploiting the cysteine sulfur as target element. Neonatal rat calvarial osteoblasts were attached to RGD-modified PPy-coated Ti substrates at levels significantly greater than on unmodified PPy-coated Ti and glass coverslip substrates.
This paper references
10.1126/SCIENCE.2821619
New perspectives in cell adhesion: RGD and integrins.
E. Ruoslahti (1987)
10.1080/15421408808083024
Electrochemically Controlled Release of Drug Ions from Conducting Polymers
L. Miller (1988)
10.1163/156856293X00519
Surface properties of RGD-peptide grafted polyurethane block copolymers: variable take-off angle and cold-stage ESCA studies.
H. Lin (1993)
10.1073/PNAS.91.8.3201
Electrically conducting polymers can noninvasively control the shape and growth of mammalian cells.
J. Wong (1994)
10.1039/F19898501685
Analytical characterization of electrode surface by X-ray photoelectron spectroscopy. β-PbO2-based cathode in voltage-compatible lithium cells
C. Malitesta (1989)
10.1002/APP.1991.070430219
Investigation of the relationship between conductivity and protein‐binding properties of polypyrrole
A. Smith (1991)
10.1016/0022-0728(88)80136-0
Preparation and application of conducting polymers containing chemically active counterions for analytical purposes
G. Wallace (1988)
10.1016/0142-9612(96)00015-4
Secretory function of adrenal chromaffin cells cultured on polypyrrole films.
T. Aoki (1996)
10.1021/BC9600271
Specific activity of polypyrrole nanoparticulate immunoreagents: comparison of surface chemistry and immobilization options.
M. Pope (1996)
10.1016/0142-9612(96)85765-6
Conditions which promote mineralization at the bone-implant interface: a model in vitro study.
K. Dee (1996)
10.1038/309030A0
Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule
M. Pierschbacher (1984)
10.1002/JBM.820290904
Photochemical surface derivatization of a peptide containing Arg-Gly-Asp (RGD).
T. Sugawara (1995)
10.1021/MA00019A010
Interaction of cationic polypeptides with electroactive polypyrrole/poly(styrenesulfonate) and poly(N-methylpyrrole)/poly(styrenesulfonate) films
L. Prezyna (1991)
10.1083/JCB.114.5.1089
An RGD spacing of 440 nm is sufficient for integrin alpha V beta 3- mediated fibroblast spreading and 140 nm for focal contact and stress fiber formation
S. Massia (1991)
10.1021/AC00109A046
Correlation between permselectivity and chemical structure of overoxidized polypyrrole membranes used in electroproduced enzyme biosensors
F. Palmisano (1995)
10.1002/(SICI)1097-4636(19970615)35:4<513::AID-JBM11>3.0.CO;2-C
Characterization and development of RGD-peptide-modified poly(lactic acid-co-lysine) as an interactive, resorbable biomaterial.
J. D. Cook (1997)
10.1163/156856298X00064
Biomolecular modification of p(AAm-co-EG/AA) IPNs supports osteoblast adhesion and phenotypic expression.
J. Bearinger (1998)
10.1002/JBM.820280307
Synthesis, surface, and cell-adhesion properties of polyurethanes containing covalently grafted RGD-peptides.
Horng-Ban Lin (1994)
10.1002/JBM.820251102
Materials for enhancing cell adhesion by immobilization of cell-adhesive peptide.
Y. Ito (1991)
10.1016/0142-9612(96)00028-2
Culture of human vascular endothelial cells on an RGD-containing synthetic peptide attached to a starch-coated polystyrene surface: comparison with fibronectin-coated tissue grade polystyrene.
J. Holland (1996)
Handbook of X-Ray Photoelectron Spectroscopy
J. Moulder (1992)
10.1016/0142-9612(92)90113-3
Endothelial cell adhesion on polyurethanes containing covalently attached RGD-peptides.
H. Lin (1992)
10.1163/156856299X00919
Development and analytical characterization of cysteine-grafted polypyrrole films electrosynthesized on Pt- and Ti-substrates as precursors of bioactive interfaces.
E. De Giglio (1999)
10.1016/0006-291X(91)91419-D
Osteoblast-osteoclast relationships in bone resorption: osteoblasts enhance osteoclast activity in a serum-free co-culture system.
A. Teti (1991)



This paper is referenced by
10.1016/J.PROGPOLYMSCI.2017.03.004
Functionalization of conducting polymers for biointerface applications
A. J. Hackett (2017)
10.1007/978-3-319-69378-1_41
Electro-Optic and Optical Devices
P. Chandrasekhar (1999)
10.1007/978-3-319-69378-1_3
Synthesis, Purification, and Chemical Modification of CNTs
P. Chandrasekhar (2018)
10.1007/978-3-319-69378-1_30
Basic Electrochemistry of CPs
P. Chandrasekhar (2018)
10.1016/j.biomaterials.2008.11.020
Arginine-glycine-aspartic acid modified rosette nanotube-hydrogel composites for bone tissue engineering.
L. Zhang (2009)
10.1177/039139880803100905
Bioactive Titanium Implant Surfaces with Bacterial Inhibition and Osteoblast Function Enhancement Properties
Z. Shi (2008)
10.1016/S0736-0266(02)00143-2
Autologous osteoblasts enhance osseointegration of porous titanium implants
K. Frosch (2003)
10.1007/978-3-319-69378-1_12
Graphene Applications in Displays and Transparent, Conductive Films/Substrates
P. Chandrasekhar (2018)
The use of hydroxyapatite-coated collars enhances osteointegrated extra-cortical bone formation and improves long term survival of distal femoral endoprostheses
V. Batta (2019)
10.1016/S0142-9612(03)00343-0
RGD modified polymers: biomaterials for stimulated cell adhesion and beyond.
U. Hersel (2003)
10.1007/978-3-319-69378-1_37
Batteries and Energy Devices
P. Chandrasekhar (2018)
10.1016/B978-1-4557-7862-1.00008-0
Cellular responses to nanoscale surface modifications of titanium implants for dentistry and bone tissue engineering applications
K. Subramanian (2012)
10.3929/ETHZ-A-004630910
Functionalized titanium surfaces for biomedical applications: physico-chemical characterization and biological in vitro evaluation
S. Tosatti (2003)
10.1504/IJBET.2012.046959
Investigation on in vivo and in vitro blood compatibility of polypyrrole-coated titanium for cardiovascular application
J. Fan (2012)
10.2217/17435889.1.4.449
Biomedical interfaces: titanium surface technology for implants and cell carriers.
M. Schuler (2006)
10.1007/s10853-013-7538-8
Surface modification of natural and synthetic hydroxyapatites powders by grafting polypyrrole
S. Yala (2013)
10.1039/C2PY20894F
An electroactive and biologically responsive hybrid conjugate based on chemical similarity
G. Fabregat (2013)
10.1007/S10856-007-3037-2
A new titanium biofunctionalized interface based on poly(pyrrole-3-acetic acid) coating: proliferation of osteoblast-like cells and future perspectives
E. Giglio (2007)
10.1002/APP.40385
MS-Monitored Conjugation of Poly(ethylene glycol) Monomethacrylate to RGD Peptides.
Oleg Bolshakov (2014)
10.1007/978-3-319-69378-1_26
Miscellaneous Applications of Graphene
P. Chandrasekhar (2018)
10.1002/ADFM.200600669
Micropatterned Polypyrrole: A Combination of Electrical and Topographical Characteristics for the Stimulation of Cells.
N. Gómez (2007)
10.1007/978-3-319-69378-1_14
CNT Applications in the Environment and in Materials Used in Separation Science
P. Chandrasekhar (2018)
Human dermal fibroblast activation under pulsed electrical stimulation via conductive fabrics : signalling pathways and potential benefit for wound healing
Y. Wang (2015)
10.1002/JBM.A.31047
Nerve growth factor-immobilized polypyrrole: bioactive electrically conducting polymer for enhanced neurite extension.
N. Gómez (2007)
10.1098/rsif.2009.0418.focus
Biological nano-functionalization of titanium-based biomaterial surfaces: a flexible toolbox
R. Beutner (2009)
10.1016/J.APSUSC.2010.07.040
Characterization of bioactive RGD peptide immobilized onto poly(acrylic acid) thin films by plasma polymerization
Hyun suk Seo (2010)
10.1002/psc.2660
Electro‐biocompatibility of conjugates designed by chemical similarity
S. Maione (2014)
10.1002/ADFM.201700905
Nanotechnology and Nanomaterials for Improving Neural Interfaces
Mian Wang (2018)
Biologically inspired Rosette nanotube nanocomposites for bone tissue engineering, orthopedic and vascular applications
L. Zhang (2009)
10.1002/mabi.200900096
Effect of motif-programmed artificial proteins on the calcium uptake in a synthetic hydrogel.
T. Chirila (2009)
10.1016/j.actbio.2009.07.008
Development and characterization of rhVEGF-loaded poly(HEMA-MOEP) coatings electrosynthesized on titanium to enhance bone mineralization and angiogenesis.
E. De Giglio (2010)
10.1007/978-3-319-69378-1_39
Displays, Including Light-Emitting Diodes (LEDs) and Conductive Films
P. Chandrasekhar (2018)
See more
Semantic Scholar Logo Some data provided by SemanticScholar