Online citations, reference lists, and bibliographies.
← Back to Search

PEO-like Plasma Polymerized Tetraglyme Surface Interactions With Leukocytes And Proteins: In Vitro And In Vivo Studies

M. Shen, L. Martinson, M. Wagner, D. Castner, B. Ratner, T. Horbett
Published 2002 · Medicine, Materials Science

Cite This
Download PDF
Analyze on Scholarcy
Share
Polyethylene oxide (PEO) surfaces reduce non-specific protein and cell interactions with implanted biomaterials and may improve their biocompatibility. PEO-like polymerized tetraglyme surfaces were made by glow discharge plasma deposition onto fluorinated ethylene propylene copolymer (FEP) substrates and were shown to adsorb less than 10 ng/cm2 of fibrinogen in vitro. The ability of the polymerized tetraglyme surfaces to resist leukocyte adhesion was studied in vitro and in vivo. Polymerized tetraglyme and FEP were implanted subcutaneously in mice and removed after 1 day or 4 weeks. Histological analysis showed a similar degree of fibrous encapsulation around all of the 4-week implants. Darkly stained wells were present in the fibrous tissues at the tissue-material interface of both FEP and tetraglyme. Scanning electron micrographs showed that in vivo macrophage adhesion to polymerized tetraglyme was much higher than to FEP. After 2-hour contact with heparinized whole blood, polymorphonuclear leukocyte (PMN) adhesion to polymerized tetraglyme was much higher than to FEP, while platelet adhesion to polymerized tetraglyme was lower than to FEP. When PMNs isolated from blood were suspended in 10% autologous plasma, cell adhesion to polymerized tetraglyme was higher than to FEP; however when the cells were suspended in heat inactivated serum, cell adhesion to FEP was higher than to polymerized tetraglyme. The surface chemistry of polymerized tetraglyme did not change after 2-hour blood contact, but displayed nitrogen functional groups after 1-day implantation and became slightly degraded after 4-week implantation. The surface chemistry of FEP did not change significantly after blood contact or implantation. Loosely bound proteins such as fibrinogen on polymerized tetraglyme may contribute to the adhesion of PMNs and macrophages and ultimately to fibrous encapsulation (the foreign body response) around the implants.
This paper references
10.1002/(SICI)1097-4636(19970915)36:4<542::AID-JBM13>3.0.CO;2-C
Hydrophilicity of polymers and soft tissue responses: a quantitative analysis.
J. Hunt (1997)
10.1002/(SICI)1097-4636(20000315)49:4<435::AID-JBM2>3.0.CO;2-Y
Adsorbed serum proteins responsible for surface dependent human macrophage behavior.
C. R. Jenney (2000)
10.1002/(SICI)1097-4636(199902)44:2<206::AID-JBM11>3.0.CO;2-D
Effects of surface-coupled polyethylene oxide on human macrophage adhesion and foreign body giant cell formation in vitro.
C. R. Jenney (1999)
10.1084/JEM.178.6.2147
Fibrin(ogen) mediates acute inflammatory responses to biomaterials
L. Tang (1993)
10.1016/S0022-2143(97)90159-1
Adhesion and activation of platelets and polymorphonuclear granulocyte cells at TiO2 surfaces.
H. Nygren (1997)
10.1021/LA990303A
Optimizing Cell−Surface Interactions by Photografting of Poly(ethylene glycol)
V. Thom (2000)
10.1002/JBM.820291208
Neovascularization of synthetic membranes directed by membrane microarchitecture.
J. Brauker (1995)
10.1067/MLC.2001.111470
Adhesion receptors of polymorphonuclear granulocytes on titanium in contact with whole blood.
C. Eriksson (2001)
10.1002/JBM.820260806
Biomaterial-induced alterations of neutrophil superoxide production.
S. Kaplan (1992)
10.1163/156856299X00720
Grafted poly(ethylene oxide) brushes as nonfouling surface coatings.
D. Leckband (1999)
The Molecular and Cellular Bioogy of Wound Repair
D.W.H. Riches (1996)
10.1002/JBM.820260402
Glow discharge plasma deposition of tetraethylene glycol dimethyl ether for fouling-resistant biomaterial surfaces.
G. López (1992)
10.1016/1054-8807(93)90054-6
Chapter 13 Principles underlying the role of adsorbed plasma proteins in blood interactions with foreign materials
T. Horbett (1993)
10.1016/0142-9612(88)90063-4
In vitro and in vivo interactions of cells with biomaterials.
N. Ziats (1988)
10.1002/1097-4636(200010)52:1<219::AID-JBM28>3.0.CO;2-F
Modulating the biocompatibility of polymer surfaces with poly(ethylene glycol): effect of fibronectin.
G. Altankov (2000)
10.1002/(SICI)1097-4636(199907)46:1<22::AID-JBM3>3.0.CO;2-R
In vitro cytotoxicity and in vivo biocompatibility of poly(propylene fumarate-co-ethylene glycol) hydrogels.
L. Suggs (1999)
10.1163/156856293X00069
Review Does polyethylene oxide possess a low thrombogenicity
G. R. Llanos (1993)
10.1002/(SICI)1097-4636(199902)44:2<130::AID-JBM2>3.0.CO;2-9
Human plasma fibrinogen adsorption and platelet adhesion to polystyrene.
W. Tsai (1999)
10.1163/156856299X00702
Surface modification with PEO-containing triblock copolymer for improved biocompatibility: in vitro and ex vivo studies.
A. Kidane (1999)
10.1002/JBM.820260605
Blood compatibility of SPUU-PEO-heparin graft copolymers.
K. D. Park (1992)
10.1172/JCI112687
Binding of fibrinogen to human monocytes.
D. Altieri (1986)
Inflammatory responses to implanted polymeric biomaterials: role of surface-adsorbed immunoglobulin G.
L. Tang (1993)
10.1002/JBM.820270702
New ideas in biomaterials science--a path to engineered biomaterials.
B. Ratner (1993)
10.1002/(SICI)1097-4636(199808)41:2<171::AID-JBM1>3.0.CO;2-F
Human monocyte/macrophage adhesion, macrophage motility, and IL-4-induced foreign body giant cell formation on silane-modified surfaces in vitro. Student Research Award in the Master's Degree Candidate Category, 24th Annual Meeting of the Society for Biomaterials, San Diego, CA, April 22-26, 1998.
C. R. Jenney (1998)
Proc
A. K. McNally
10.1016/0142-9612(84)90060-7
Biomaterial biocompatibility and the macrophage.
J. Anderson (1984)
10.1163/156856201753252507
Inhibition of monocyte adhesion and fibrinogen adsorption on glow discharge plasma deposited tetraethylene glycol dimethyl ether
M. Shen (2001)
10.1163/156856200744228
In vitro and in vivo studies of PEO-grafted blood-contacting cardiovascular prostheses
K. Park (2000)
10.1002/JCB.240560206
Polymer substrates for controlled biological interactions
L. Cima (1994)
10.1111/j.1749-6632.1997.tb52191.x
Fibrinogen‐dependent Adherence of Macrophages to Surfaces Coated with Poly(ethylene oxide)/Poly(propylene oxide) Triblock Copolymers a
S. O'connor (1997)
10.1002/SIA.740160185
Chemical reactions induced in polymers by keV ions, electrons and photons
G. Marletta (1990)
10.1073/PNAS.91.21.10119
Complement C3 participation in monocyte adhesion to different surfaces.
A. K. McNally (1994)
10.1002/1097-4636(20011205)57:3<336::AID-JBM1176>3.0.CO;2-E
The effects of surface chemistry and adsorbed proteins on monocyte/macrophage adhesion to chemically modified polystyrene surfaces.
M. Shen (2001)
Occupancy of CD11b/CD18 (Mac-1) divalent ion binding site(s) induces leukocyte adhesion.
D. Altieri (1991)
10.1172/JCI119267
A MAC-1 attack: integrin functions directly challenged in knockout mice.
E. Plow (1997)
10.1172/JCI106664
Isolation and properties of phagocytic vesicles from polymorphonuclear leukocytes.
T. P. Stossel (1971)
10.1002/(SICI)1097-4636(199601)30:1<31::AID-JBM5>3.0.CO;2-S
In vivo biocompatibility study of ABA triblock copolymers consisting of poly(L-lactic-co-glycolic acid) A blocks attached to central poly(oxyethylene) B blocks.
B. Ronneberger (1996)
10.1002/(SICI)1097-4636(200001)49:1<25::AID-JBM4>3.0.CO;2-I
The role of superoxide ions in the degradation of synthetic absorbable sutures.
K. Lee (2000)
10.1002/JBM.820250205
Foreign-body giant cells and polyurethane biostability: in vivo correlation of cell adhesion and surface cracking.
Q. Zhao (1991)



This paper is referenced by
10.1146/ANNUREV.BIOENG.5.040202.121615
The tissue engineeting puzzle: a molecular perspective.
V. Vogel (2003)
10.1002/ANGE.201712448
Engineering von Proteinen an Oberflächen: Von komplementärer Charakterisierung zu Materialoberflächen mit maßgeschneiderten Funktionen
S. Morsbach (2018)
10.1039/d0tb00051e
Surfaces immobilized with oligo-prolines prevent protein adsorption and cell adhesion.
Yuri Noguchi (2020)
10.1021/acsami.5b10811
Zwitterionic-Modified Starch-Based Stealth Micelles for Prolonging Circulation Time and Reducing Macrophage Response.
L. Ye (2016)
10.1016/j.biomaterials.2011.06.006
Uniform zwitterionic polymer hydrogels with a nonfouling and functionalizable crosslinker using photopolymerization.
Louisa R. Carr (2011)
10.1016/B978-0-12-803581-8.00523-3
Biocompatibility of Microsystems
T. Singh (2008)
10.1016/J.APSUSC.2018.04.184
Synthesis of efficient bacterial adhesion-resistant coatings by one-step polydopamine-assisted deposition of branched polyethylenimine- g -poly(sulfobetaine methacrylate) copolymers
B. Ran (2018)
10.1002/JBM.A.30906
Plasma deposition of tetraglyme inside small diameter tubing: optimization and characterization.
L. Cao (2007)
10.1039/c1nr10173k
Stabilization and functionalization of iron oxide nanoparticles for biomedical applications.
E. Amstad (2011)
10.1201/b13758-8
Application of polymer drugs to medical devices and reparative medicine
M. Aguilar (2013)
10.1002/chem.201905326
Facile Fabrication of Bio‐ and Dual‐Functional Poly(2‐oxazoline) Bottle‐Brush Brush Surfaces
Yunhao Du (2019)
10.1002/APP.24127
Adsorption of fibrinogen onto macroporous, biocompatible sponges based on poly(2‐hydroxyethyl methacrylate)
A. Bajpai (2006)
10.1002/adma.200901407
Ultralow-fouling, functionalizable, and hydrolyzable zwitterionic materials and their derivatives for biological applications.
S. Jiang (2010)
10.1039/b923789p
Microstructured poly(2-oxazoline) bottle-brush brushes on nanocrystalline diamond.
Naima A. Hutter (2010)
10.3724/SP.J.1105.2014.13164
Molecular Dynamics Simulation of Interaction between Lysozyme and Non-fouling Polymer Membranes: Molecular Dynamics Simulation of Interaction between Lysozyme and Non-fouling Polymer Membranes
Zhang Heng (2014)
10.1680/SUFI.14.00008
Low and atmospheric plasma polymerisation of nanocoatings for bio-applications
Sudhir Bhatt (2015)
10.1002/JBM.A.31270
Monocyte activation in response to polyethylene glycol hydrogels grafted with RGD and PHSRN separated by interpositional spacers of various lengths.
D. Schmidt (2007)
10.1002/jbm.a.36460
Fibrinogen adsorption to biomaterials.
T. Horbett (2018)
10.1002/PPAP.200400083
Soft plasma treated surfaces: Tailoring of structure and properties for biomaterial applications
R. Foerch (2005)
10.1002/jbm.a.32954
Complement activation on poly(ethylene oxide)-like radiofrequency glow discharge-deposited surfaces.
L. M. Szott (2011)
10.1016/J.PORGCOAT.2014.07.011
Zwitterionic siloxane-polyurethane fouling-release coatings
Rajan B. Bodkhe (2015)
10.1093/rb/rbv003
Drug carriers based on highly protein-resistant materials for prolonged in vivo circulation time
Ruiyuan Liu (2015)
10.1016/S0142-9612(02)00612-9
Characterizing multicomponent adsorbed protein films using electron spectroscopy for chemical analysis, time-of-flight secondary ion mass spectrometry, and radiolabeling: capabilities and limitations.
M. Wagner (2003)
10.1021/am200690s
Evaluation of photochemically immobilized poly(2-ethyl-2-oxazoline) thin films as protein-resistant surfaces.
H. Wang (2011)
10.1016/j.actbio.2019.09.043
Inflammation via myeloid differentiation primary response gene 88 signaling mediates the fibrotic response to implantable synthetic poly(ethylene glycol) hydrogels.
Luke D. Amer (2019)
10.1002/9783527638482.CH28
Self‐Assembled Multifunctional Polymers for Biointerfaces
G. Coullerez (2011)
10.7939/R3891249S
Human Plasma Adsorption to Biomaterials: Fundamental Level Chemical Modifications and Their Effects on Biocompatibility
Markian S Bahniuk (2017)
10.3390/POLYM7111518
Switchable Materials Containing Polyzwitterion Moieties
Markéta Ilčíková (2015)
10.1063/1.3012563
A molecular simulation study of methylated and hydroxyl sugar-based self-assembled monolayers: Surface hydration and resistance to protein adsorption.
Jason C. Hower (2008)
10.1039/B703416B
Current strategies towards hemocompatible coatings
C. Werner (2007)
10.1021/am302208f
Fabrication and characterization of plasma-polymerized poly(ethylene glycol) film with superior biocompatibility.
Changrok Choi (2013)
10.1016/j.mtchem.2019.100227
Latest advances in zwitterionic structures modified dialysis membranes
A. Mollahosseini (2020)
See more
Semantic Scholar Logo Some data provided by SemanticScholar