Online citations, reference lists, and bibliographies.

Rabbit Study Of An In Situ Forming Hydrogel Vitreous Substitute.

Katelyn E Swindle-Reilly, Milan Shah, Paul D. Hamilton, Thomas A. Eskin, Shalesh Kaushal, Nathan Ravi
Published 2009 · Biology, Medicine
Cite This
Download PDF
Analyze on Scholarcy
Share
PURPOSE An in situ forming hydrogel was evaluated as a potential vitreous substitute in rabbits. METHODS The hydrogel used a disulfide cross-linker that was then reduced to produce an injectable thiol-containing polymer solution. The disulfide cross-links reformed by air oxidation of the thiols and produced a stable hydrogel once inside the eye. The polymer was clear, autoclavable, and could be stored easily in the presence of nitrogen gas. Capillary rheometry was used to measure the viscoelastic properties of the hydrogels and the porcine vitreous. Fourteen black rabbits underwent a pars plana, 25-gauge, three-port vitrectomy by a single surgeon with injection of a vitreous substitute. RESULTS The refractive indices of the hydrogels were measured by refractometry and were shown to be close to 1.33, and the 2% hydrogel matched the mechanical properties of the natural vitreous humor. The reduced polymeric hydrogel was easily injectable through a small-gauge needle into the vitreous cavity and did not show any fragmentation. The material underwent gelation within the eye, remained optically clear, and appeared well tolerated clinically. Slit lamp examination, dilated fundus examination, and electroretinograms showed no evidence of vitritis, uveitis, or endophthalmitis after 1 week. Histopathologic evaluation did not reveal any overt toxicity or gross morphologic changes in the retina. CONCLUSIONS The fact that this process of in situ gelation gives rise to hydrogels that are biocompatible and physically and optically similar to the natural vitreous suggests its suitability as a permanent vitreous substitute. Hydrogel candidates will be further studied to evaluate long-term biocompatibility and degradation in vivo.
This paper references
10.1586/17469899.2.2.255
Recent advances in polymeric vitreous substitutes
K. E. Swindle (2007)
10.1002/(SICI)1097-4636(19980315)39:4<650::AID-JBM21>3.0.CO;2-9
Biodegradation in vitro and retention in the rabbit eye of crosslinked poly(1-vinyl-2-pyrrolidinone) hydrogel as a vitreous substitute.
Yi Chun Hong (1998)
Alloplastic vitreous replacement with acrylamide. A preliminary report.
K. Müller-Jensen (1974)
10.1038/sj.eye.6702875
A new strategy to replace the natural vitreous by a novel capsular artificial vitreous body with pressure-control valve
Qianying Gao (2008)
10.1002/masy.200550918
Internal Tension: A Novel Hypothesis Concerning the Mechanical Properties of the Vitreous Humor
Charles S. Nickerson (2005)
Biocompatibility of polyvinylalcohol gel as a vitreous substitute.
Shinji Maruoka (2006)
10.1016/j.survophthal.2007.02.004
Biocompatibility assessment of liquid artificial vitreous replacements: relevance of in vitro studies.
Andrea Matteucci (2007)
Morphology and ultrastructure of human vitreous fibers.
Julien Sebag (1989)
10.1016/S1350-9462(99)00016-6
Structural macromolecules and supramolecular organisation of the vitreous gel
Paul N. Bishop (2000)
10.1001/archopht.1968.00980050122020
Glyceryl methacrylate hydrogel as a vitreous implant. An experimental study.
Salvatore Daniele (1968)
Ocular toxicity of low-molecular-weight components of silicone and fluorosilicone oils.
Kimitoshi Nakamura (1991)
10.1163/156856296X00453
Poly(1-vinyl-2-pyrrolidinone) hydrogels as vitreous substitutes: histopathological evaluation in the animal eye.
Sarojini Vijayasekaran (1996)
10.1016/S0079-6700(97)00045-2
THE USE OF HYDROPHILIC POLYMERS AS ARTIFICIAL VITREOUS
Traian V Chirila (1998)
10.1002/(SICI)1097-0126(199807)46:3<183::AID-PI941>3.0.CO;2-9
Poly(1-vinyl-2-pyrrolidinone) hydrogels as vitreous substitutes: a rheological study
Traian V Chirila (1998)
10.1097/00006982-199004000-00012
EVALUATION OF A VISCOELASTIC SOLUTION OF HYDROXYPROPYL METHYLCELLULOSE AS A POTENTIAL VITREOUS SUBSTITUTE
Miguel Fernández Refojo (1990)
10.1097/00006982-199515010-00002
INTRAOCULAR TOLERANCE OF PERFLUOROOCTYLBROMIDE (PERFLUBRON)
M. Flores-Aguilar (1995)
10.1021/bm049574c
Refilling of ocular lens capsule with copolymeric hydrogel containing reversible disulfide.
Hyder Aliyar (2005)
[An attempt to replace the vitreous body by polyacrylamide].
K. Müller-Jensen (1968)
10.1136/bjo.57.8.542
Intravitreal silicone injection in retinal detachment.
Jack J. Kanski (1973)
10.1002/jbm.a.31769
In situ formation of hydrogels as vitreous substitutes: Viscoelastic comparison to porcine vitreous.
K. E. Swindle (2008)
10.1177/0883911506064368
Internal Osmotic Pressure as a Mechanism of Retinal Attachment in a Vitreous Substitute
William J Foster (2006)
10.3233/BIR-1984-21602
Dynamic viscoelasticity of bovine vitreous body.
Masayuki Tokita (1984)
Artificial vitreous replacements.
Narendra Amalendu Soman (2003)
10.1007/BF02160337
Age-related changes in human vitreous structure
J Sebag (2005)
10.1016/S0002-9394(01)01295-8
Use of perfluorohexyloctane as a long-term internal tamponade agent in complicated retinal detachment surgery.
Bernd Kirchhof (2002)



This paper is referenced by
10.1002/APP.41280
In Vitro evaluation and dissipative particle dynamics simulation of PLGA‐PEG‐PLGA
Yang Cao (2015)
10.3389/fbioe.2018.00199
Rheological Properties and Age-Related Changes of the Human Vitreous Humor
Nguyen Khoi Tram (2018)
10.1007/978-1-4939-1086-1_6
I.F. Vitreous Biochemistry and Artificial Vitreous
Sven Crafoord (2014)
10.1016/j.addr.2019.04.007
Thiolated polymers: Bioinspired polymers utilizing one of the most important bridging structures in nature.
Christina Leichner (2019)
10.1002/advs.201800711
Antifouling Super Water Absorbent Supramolecular Polymer Hydrogel as an Artificial Vitreous Body
Hongbo Wang (2018)
10.1007/978-3-319-19434-9_2
Animal Models of Cataracts
Judith A West-Mays (2016)
10.1002/jbm.b.33958
Characterization of PVA/glutaraldehyde hydrogels obtained using Central Composite Rotatable Design (CCRD).
Andreia de Araújo Morandim-Giannetti (2018)
10.1038/S41551-017-0044
Fast-forming hydrogel with ultralow polymeric content as an artificial vitreous body
Kaori Hayashi (2017)
10.1007/978-1-4939-1086-1_26
IV.B. Oxygen in Vitreoretinal Physiology and Pathology
Nancy Holekamp (2014)
10.1177/0883911516688482
Poly(acrylamide co-acrylic acid) for use as an in situ gelling vitreous substitute
Joshua Davis (2017)
10.1007/978-1-4419-9920-7_12
Hydrogels for Ocular Posterior Segment Drug Delivery
Gauri P. Misra (2011)
10.3389/fbioe.2019.00044
Erratum: Rheological Properties and Age-Related Changes of the Human Vitreous Humor
Frontiers Production Office (2019)
10.1016/B978-0-08-100147-9.00005-5
Current concepts in the design of hydrogels as vitreous substitutes
Katelyn E Swindle-Reilly (2016)
10.1155/2017/3172138
Vitreous Substitutes: Old and New Materials in Vitreoretinal Surgery
Camilla Alovisi (2017)
10.1016/B978-2-294-71471-9.50030-8
Chapitre 30 – Perspectives techniques et innovations
Francine Béhar-Cohen (2011)
10.1021/ACS.MACROMOL.6B00885
Development of a Vitreous Substitute: Incorporating Charges and Fibrous Structures in Synthetic Hydrogel Materials
Svetlana Morozova (2016)
10.1016/J.MTCOMM.2019.05.012
Chemical cross-linking methods for cell encapsulation in hydrogels
Cécile Echalier (2019)
10.1002/ADEM.201080104
In Situ Forming Hydrogels: A Thermo‐Responsive Polyelectrolyte as Promising Liquid Artificial Vitreous Body Replacement
Falko Strotmann (2011)
10.1002/marc.201300818
Designing injectable, covalently cross-linked hydrogels for biomedical applications.
Mathew Patenaude (2014)
10.1016/j.survophthal.2010.09.001
Vitreous substitutes: a comprehensive review.
Teri T. Kleinberg (2011)
10.1167/iovs.17-21536
A Self-Assembling Peptide Gel as a Vitreous Substitute: A Rabbit Study.
Koji Uesugi (2017)
Highly Shear-Thinning Mucoadhesive Hydrogels for Ophthalmic Applications
Paniz Sheikholeslami (2013)
10.1002/mabi.201200384
An anti-angiogenic reverse thermal gel as a drug-delivery system for age-related wet macular degeneration.
Daewon Park (2013)
Biomaterials for protection and repair of the central nervous system
Christopher D. Pritchard (2012)
10.1097/IAE.0b013e3182618d2e
SAFETY OF MEDIUM-CHAIN TRIGLYCERIDES USED AS AN INTRAOCULAR TAMPONADING AGENT IN AN EXPERIMENTAL VITRECTOMY MODEL RABBIT
Sylvain Auriol (2013)
10.1016/j.actbio.2016.07.051
Biomimetic hydrogel with tunable mechanical properties for vitreous substitutes.
Sruthi Santhanam (2016)
10.1016/j.ejpb.2015.05.016
Hydrogels in ophthalmic applications.
Susanne Kirchhof (2015)
10.1038/srep01838
A Novel Vitreous Substitute of Using a Foldable Capsular Vitreous Body Injected with Polyvinylalcohol Hydrogel
Songfu Feng (2013)
10.1017/JFM.2011.263
Oscillatory motion of a viscoelastic fluid within a spherical cavity
Julia Meskauskas (2011)
10.1002/mabi.201900305
A Hydrogel Vitreous Substitute that Releases Antioxidant.
Nguyen Khoi Tram (2019)
10.1155/2014/351804
Vitreous Substitutes: The Present and the Future
Simone Donati (2014)
10.1002/APP.35584
Rheological properties of PLGA‐PEG‐PLGA copolymers for ophthalmic injection
Bochu Wang (2012)
See more
Semantic Scholar Logo Some data provided by SemanticScholar