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Effects Of Drought Stress And Water Recovery On Physiological Responses And Gene Expression In Maize Seedlings

Xiangbo Zhang, Lei Lei, Jinsheng Lai, H. Zhao, W. Song
Published 2018 · Biology, Medicine

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BackgroundDrought is one of the major factors limiting global maize production. Exposure to long-term drought conditions inhibits growth and leads to yield losses. Although several drought-responsive genes have been identified and functionally analyzed, the mechanisms underlying responses to drought and water recovery treatments have not been fully elucidated. To characterize how maize seedling respond to drought stress at the transcriptional level, we analyzed physiological responses and differentially expressed genes (DEGs) in the inbred line B73 under water deficit and recovery conditions.ResultsThe data for relative leaf water content, leaf size, and photosynthesis-related parameters indicated that drought stress significantly repressed maize seedling growth. Further RNA sequencing analysis revealed that 6107 DEGs were responsive to drought stress and water recovery, with more down-regulated than up-regulated genes. Among the DEGs, the photosynthesis- and hormone-related genes were enriched in responses to drought stress and re-watering. Additionally, transcription factor genes from 37 families were differentially expressed among the three analyzed time-points. Gene ontology enrichment analyses of the DEGs indicated that 50 GO terms, including those related to photosynthesis, carbohydrate metabolism, oxidoreductase activities, nutrient metabolism and other drought-responsive pathways, were over-represented in the drought-treated seedlings. The content of gibberellin in drought treatment seedlings was decreased compared to that of control seedlings, while abscisic acid showed accumulated in the drought treated plants. The deep analysis of DEGs related to cell wall development indicated that these genes were prone to be down-regulated at drought treatment stage.ConclusionsMany genes that are differentially expressed in responses to drought stress and water recovery conditions affect photosynthetic systems and hormone biosynthesis. The identified DEGs, especially those encoding transcription factors, represent potential targets for developing drought-tolerant maize lines.
This paper references
Breeding for drought and nitrogen stress tolerance in maize: From theory to practice
Marianne Bänziger (2000)
Analysis of Gene Expression and Physiological Responses in Three Mexican Maize Landraces under Drought Stress and Recovery Irrigation
C. Hayano-Kanashiro (2009)
Physiological and biochemical mechanisms associated with trehalose-induced copper-stress tolerance in rice
M. Mostofa (2015)
Overexpression of Arabidopsis NLP7 improves plant growth under both nitrogen-limiting and -sufficient conditions by enhancing nitrogen and carbon assimilation
Lin-Hui Yu (2016)
Photosynthesis: ancient, essential, complex, diverse … and in need of improvement in a changing world.
Ü. Niinemets (2017)
Leaf Transpiration Efficiency of Some Drought-Resistant Maize Lines
J. Bunce (2010)
The nodule inception-like protein 7 modulates nitrate sensing and metabolism in Arabidopsis.
Loren Castaings (2009)
Nitrogen and carbon nutrient and metabolite signaling in plants.
G. Coruzzi (2001)
A transposable element in a NAC gene is associated with drought tolerance in maize seedlings
H. Mao (2015)
Effects of Drought on Gene Expression in Maize Reproductive and Leaf Meristem Tissue Revealed by RNA-Seq1[W][OA]
Akshay Kakumanu (2012)
Abiotic stress, the field environment and stress combination.
R. Mittler (2006)
Genetic variation in ZmVPP1 contributes to drought tolerance in maize seedlings
Xianglan Wang (2016)
ZmGOLS2, a target of transcription factor ZmDREB2A, offers similar protection against abiotic stress as ZmDREB2A
L. Gu (2015)
Making better maize plants for sustainable grain production in a changing climate
Fangping Gong (2015)
Photosynthesis: ancient, essential, complex, diverse
U Niinemets (2017)
TopHat: discovering splice junctions with RNA-Seq
C. Trapnell (2009)
Plant nuclear factor Y (NF-Y) B subunits confer drought tolerance and lead to improved corn yields on water-limited acres
D. E. Nelson (2007)
Current advances in the investigation of leaf rolling caused by biotic and abiotic stress factors.
Asım Kadıoğlu (2012)
Research advances in major cereal crops for adaptation to abiotic stresses.
Rk Maiti (2014)
Transcriptomic complexity in young maize primary roots in response to low water potentials
Nina Opitz (2014)
Is Change in Ovary Carbon Status a Cause or a Consequence of Maize Ovary Abortion in Water Deficit during Flowering?1[OPEN]
V. Oury (2016)
Plant Productivity and Environment
J. Boyer (1982)
Corrigendum: Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks
C. Trapnell (2014)
Trehalose pretreatment induces salt tolerance in rice (Oryza sativa L.) seedlings: oxidative damage and co-induction of antioxidant defense and glyoxalase systems
M. Mostofa (2014)
Deletion of an Endoplasmic Reticulum Stress Response Element in a ZmPP2C-A Gene Facilitates Drought Tolerance of Maize Seedlings.
Yanli Xiang (2017)
Transcriptome Profile Analysis of Maize Seedlings in Response to High-salinity, Drought and Cold Stresses by Deep Sequencing
X. Shan (2013)
Expanding knowledge of the Rubisco kinetics variability in plant species: environmental and evolutionary trends.
J. Galmés (2014)
Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell.
M. Chaves (2009)
Genome-Wide Identification of VQ Motif-Containing Proteins and their Expression Profiles Under Abiotic Stresses in Maize
Weibin Song (2016)
Protection of the Photosynthetic Apparatus from Extreme Dehydration and Oxidative Stress in Seedlings of Transgenic Tobacco
C. Almoguera (2012)
Plant responses to water deficit
E. Bray (1997)
Cell Wall Metabolism in Response to Abiotic Stress
Hyacinthe Le Gall (2015)
Guard cell sensory systems: recent insights on stomatal responses to light, abscisic acid, and CO2.
Sarah M. Assmann (2016)
Tandem CCCH zinc finger proteins in plant growth, development and stress response.
Srimathi Bogamuwa (2014)
Hydrogen peroxide as a signal controlling plant programmed cell death
T. Gechev (2005)
Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses.
K. Yamaguchi-Shinozaki (2006)
Drought acclimation reduces O2*- accumulation and lipid peroxidation in wheat seedlings.
Devarshi S. Selote (2004)
Functional analysis of the 5' regulatory region of the maize GALACTINOL SYNTHASE2 gene.
L. Gu (2013)
Environmental perception avenues: the interaction of cytokinin and environmental response pathways.
Cristiana T. Argueso (2009)
Molecular mechanisms of desiccation tolerance in resurrection plants
T. Gechev (2012)
The reduction in maize leaf growth under mild drought affects the transition between cell division and cell expansion and cannot be restored by elevated gibberellic acid levels
H. Nelissen (2018)
Overexpression of the Maize psbA Gene Enhances Drought Tolerance Through Regulating Antioxidant System, Photosynthetic Capability, and Stress Defense Gene Expression in Tobacco
Yongjin Huo (2016)
Rubisco Catalytic Properties and Temperature Response in Crops1
C. Hermida-Carrera (2016)
Cloning and characterization of a maize bZIP transcription factor, ZmbZIP72, confers drought and salt tolerance in transgenic Arabidopsis
Sheng Ying (2011)
Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks
C. Trapnell (2012)
Regulatory metabolic networks in drought stress responses.
M. Seki (2007)
Identification of Drought Tolerant Mechanisms in Maize Seedlings Based on Transcriptome Analysis of Recombination Inbred Lines
Haowei Min (2016)
MAPMAN: a user-driven tool to display genomics data sets onto diagrams of metabolic pathways and other biological processes.
O. Thimm (2004)
Molecular and physiological approaches to maize improvement for drought tolerance.
W. Bruce (2002)
Ribosome profiling reveals dynamic translational landscape in maize seedlings under drought stress.
Lei Lei (2015)
Regulation and functional analysis of ZmDREB2A in response to drought and heat stresses in Zea mays L.
F. Qin (2007)
Expression of the maize GALACTINOL SYNTHASE gene family: (II) Kernel abscission, environmental stress and myo‐inositol influences accumulation of transcript in developing seeds and callus cells
Tianyong Zhao (2004)
Improving drought tolerance in maize: a view from industry
H. Campos (2004)
THE WATER-WATER CYCLE IN CHLOROPLASTS: Scavenging of Active Oxygens and Dissipation of Excess Photons.
K. Asada (1999)
Expression of trehalose-6-phosphate phosphatase in maize ears improves yield in well-watered and drought conditions
Michael L Nuccio (2015)
Genotypic Variation in Growth and Physiological Response to Drought Stress and Re-Watering Reveals the Critical Role of Recovery in Drought Adaptation in Maize Seedlings
Daoqian Chen (2016)
Enhancement of Plant Productivity in the Post-Genomics Era
N. P. Thảo (2016)
Response of plants to water stress
Y. Osakabe (2014)
Drought stress in maize causes differential acclimation responses of glutathione and sulfur metabolism in leaves and roots
N. Ahmad (2016)

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Joaquina Farias (2020)
Adapting Cereal Grain Crops to Drought Stress: 2020 and Beyond
Tinashe Zenda (2020)
Molecular cloning and functional analysis of 4-Coumarate:CoA ligase 4(4CL-like 1)from Fraxinus mandshurica and its role in abiotic stress tolerance and cell wall synthesis
Xiaohui Chen (2019)
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Shuangjie Jia (2020)
Mild water stress-induced priming enhance tolerance to Rosellinia necatrix in susceptible avocado rootstocks
Elsa Martínez-Ferri (2019)
A transcriptomic analysis reveals the adaptability of the growth and physiology of immature tassel to long-term soil water deficit in Zea mays L.
H. Li (2020)
Physiological, biochemical and gene-expressional responses to water deficit in apple subjected to partial root-zone drying (PRD).
Hajar Ghafari (2020)
Characterization of floral morphoanatomy and identification of marker genes preferentially expressed during specific stages of cotton flower development
S. M. de Moura (2020)
Epigenetic signatures of stress adaptation and flowering regulation in response to extended drought and recovery in Zea mays.
C. Forestan (2019)
Transcriptomic Analysis Reveals the Temporal and Spatial Changes in Physiological Process and Gene Expression in Common Buckwheat (Fagopyrum esculentum Moench) Grown under Drought Stress
Zehao Hou (2019)
Sulfur dioxide enhance drought tolerance of wheat seedlings through H2S signaling.
Lihong Li (2020)
Comparative Proteomic and Morpho-Physiological Analyses of Maize Wild-Type Vp16 and Mutant vp16 Germinating Seed Responses to PEG-Induced Drought Stress
Songtao Liu (2019)
An endophyte from salt-adapted Pokkali rice confers salt-tolerance to a salt-sensitive rice variety and targets a unique pattern of genes in its new host
Megha H. Sampangi-Ramaiah (2020)
Transcriptome-IPMS analysis reveals a tissue-dependent miR156/SPL13 regulatory mechanism in alfalfa drought tolerance
B. Feyissa (2020)
Moisture stress induced changes in root anatomy and antioxidants level in maize (Zea mays L.)
Mandeep Kaur (2020)
Comparing control options for time‐series RNA sequencing experiments in nonmodel organisms: An example from grasses
Fan Qiu (2020)
Comparative Proteomic and Physiological Analyses of Two Divergent Maize Inbred Lines Provide More Insights into Drought-Stress Tolerance Mechanisms
Tinashe Zenda (2018)
Osmoregulators’ accumulation minimizes the effects of drought stress in sugarcane and contributes to the recovery of photochemical efficiency in photosystem II after rewatering
Sebastião Oliveira Maia Júnior (2020)
Genetic interaction and inheritance of biochemical traits can predict tolerance of hybrid maize cv. SC704 to drought
Mozhgan Shirinpour (2020)
Comparative transcriptomic and physiological analyses of contrasting hybrid cultivars ND476 and ZX978 identify important differentially expressed genes and pathways regulating drought stress tolerance in maize
G. Liu (2020)
Abiotic stress and self-destruction: ZmATG8 and ZmATG12 gene transcription and osmotic stress responses in maize
Luis Herminio Chairez Tejeda (2020)
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