Online citations, reference lists, and bibliographies.

Osteoporosis Imaging: Effects Of Bone Preservation On MDCT-based Trabecular Bone Microstructure Parameters And Finite Element Models

T. Baum, E. G. Garcia, R. Burgkart, O. Gordijenko, H. Liebl, P. Jungmann, M. Gruber, T. Zahel, E. Rummeny, S. Waldt, J. Bauer
Published 2015 · Medicine, Computer Science

Cite This
Download PDF
Analyze on Scholarcy
BackgroundOsteoporosis is defined as a skeletal disorder characterized by compromised bone strength due to a reduction of bone mass and deterioration of bone microstructure predisposing an individual to an increased risk of fracture. Trabecular bone microstructure analysis and finite element models (FEM) have shown to improve the prediction of bone strength beyond bone mineral density (BMD) measurements. These computational methods have been developed and validated in specimens preserved in formalin solution or by freezing. However, little is known about the effects of preservation on trabecular bone microstructure and FEM. The purpose of this observational study was to investigate the effects of preservation on trabecular bone microstructure and FEM in human vertebrae.MethodsFour thoracic vertebrae were harvested from each of three fresh human cadavers (n = 12). Multi-detector computed tomography (MDCT) images were obtained at baseline, 3 and 6 month follow-up. In the intervals between MDCT imaging, two vertebrae from each donor were formalin-fixed and frozen, respectively. BMD, trabecular bone microstructure parameters (histomorphometry and fractal dimension), and FEM-based apparent compressive modulus (ACM) were determined in the MDCT images and validated by mechanical testing to failure of the vertebrae after 6 months.ResultsChanges of BMD, trabecular bone microstructure parameters, and FEM-based ACM in formalin-fixed and frozen vertebrae over 6 months ranged between 1.0–5.6 % and 1.3–6.1 %, respectively, and were not statistically significant (p > 0.05). BMD, trabecular bone microstructure parameters, and FEM-based ACM as assessed at baseline, 3 and 6 month follow-up correlated significantly with mechanically determined failure load (r = 0.89–0.99; p < 0.05). The correlation coefficients r were not significantly different for the two preservation methods (p > 0.05).ConclusionsFormalin fixation and freezing up to six months showed no significant effects on trabecular bone microstructure and FEM-based ACM in human vertebrae and may both be used in corresponding in-vitro experiments in the context of osteoporosis.
This paper references
A protocol system for testing biohazardous materials in an impact biomechanics research facility.
S. Duma (1999)
The effect of different storage methods on the mechanical properties of trabecular bone.
F. Linde (1993)
2000: highlights of the conference
Osteoporosis imaging: state of the art and advanced imaging.
T. Link (2012)
A patient-specific finite element methodology to predict damage accumulation in vertebral bodies under axial compression, sagittal flexion and combined loads
Y. Chevalier (2008)
Reviews and Notes: Assessment of Fracture Risk and Its Application to Screening for Postmenopausal Osteoporosis
L. Alexeeva (1995)
QCT-based finite element models predict human vertebral strength in vitro significantly better than simulated DEXA
E. Dall’Ara (2011)
Highlights of the Conference
F. Dickinson (1962)
Effects of three different preservation methods on the mechanical properties of human and bovine cortical bone.
Unger Stefan (2010)
Formalin fixation strongly influences biomechanical properties of the spine.
H. Wilke (1996)
Effects of tissue preservation on murine bone mechanical properties.
A. Nazarian (2009)
Finite element models predict in vitro vertebral body compressive strength better than quantitative computed tomography.
R. Crawford (2003)
Effect of long-term preservation on the mechanical properties of cortical bone in goats
E. V. van Haaren (2008)
NIH Consensus Development Panel on Osteoporosis Prevention, Diagnosis, and Therapy, March 7-29, 2000: highlights of the conference.
Hard tissue biochemistry: A comparison of fresh-frozen and formalin-fixed tissue samples
A. Boskey (1982)
Effects of three different preservation methods on the mechanical properties of human and bovine cortical bone.
Stefan Unger (2010)
Accurate assessment of precision errors: How to measure the reproducibility of bone densitometry techniques
C. Glüer (2005)
Effect of Fixation, Soft-Tissues, and Scan Projection on Bone Mineral Measurements with Dual Energy X-ray Absorptiometry (DXA)
E. Lochmüller (2001)
Bone histomorphometry: Standardization of nomenclature, symbols, and units: Report of the asbmr histomorphometry nomenclature committee
A. Parfitt (1987)
Formalin fixation effects on vertebral bone density and failure mechanics: an in-vitro study of human and sheep vertebrae.
S. Edmondston (1994)
Estimation of material properties in the equine metacarpus with use of quantitative computed tomography
C. Les (1994)
Quantitative computed tomography.
J. Adams (2009)
Effects of three different preservation methods on the mechanical properties of human and bovine cortical
A Nazarian (2010)
Quantitative computed tomography estimates of the mechanical properties of human vertebral trabecular bone
D. Kopperdahl (2002)
Comparison of in situ and in vitro CT scan-based finite element model predictions of proximal femoral fracture load.
J. Keyak (2003)
Relation between fractures and mortality: results from the Canadian Multicentre Osteoporosis Study
G. Ioannidis (2009)
High-resolution bone imaging for osteoporosis diagnostics and therapy monitoring using clinical MDCT and MRI.
Thomas Baum (2013)
Predicting the compressive mechanical behavior of bone.
T. Keller (1994)
Automated 3D trabecular bone structure analysis of the proximal femur—prediction of biomechanical strength by CT and DXA
T. Baum (2009)
Bone mineral density thresholds for pharmacological intervention to prevent fractures.
E. Siris (2004)
Incidence and Economic Burden of Osteoporosis‐Related Fractures in the United States, 2005–2025
R. Burge (2007)
Trabecular bone structure analysis of the spine using clinical MDCT: can it predict vertebral bone strength?
T. Baum (2013)
Assessment of fracture risk and its application to screening for postmenopausal osteoporosis (WHO Technical Report Series No 843)
J. Compston (1995)
The effects of embalming using a 4% formalin solution on the compressive mechanical properties of human cortical bone.
C. Ohman (2008)
Fracture incidence and association with bone mineral density in elderly men and women: the Rotterdam Study.
S. C. Schuit (2004)
Assessment of fracture risk and its application to screening for postmenopausal osteoporosis. Report of a WHO Study Group.
L. Alexeeva (1994)

This paper is referenced by
Effect of radiation dose reduction on texture measures of trabecular bone microstructure: an in vitro study
Muthu Rama Krishnan Mookiah (2017)
Associations between fluoride intakes, bone outcomes and dental fluorosis
R. Oweis (2018)
Correlation between vertebral bone microstructure and estimated strength in elderly women: An ex-vivo HR-pQCT study of cadaveric spine.
S. Yamada (2019)
Does formalin fixation influence MSCT/CBCT accuracy?
A. Seidel (2017)
Relationship between the mineral content of human trabecular bone and selected parameters determined from fatigue test at stepwise-increasing amplitude.
A. Mazurkiewicz (2017)
Feasibility of opportunistic osteoporosis screening in routine contrast-enhanced multi detector computed tomography (MDCT) using texture analysis
Muthu Rama Krishnan Mookiah (2017)
Association of bone mineral density with bone texture attributes extracted using routine magnetic resonance imaging
J. G. Maciel (2020)
Effect of Statistically Iterative Image Reconstruction on Vertebral Bone Strength Prediction Using Bone Mineral Density and Finite Element Modeling: A Preliminary Study
D. Anitha (2019)
The Effect of Formalin Preservation Time and Temperature on the Material Properties of Bovine Femoral Cortical Bone Tissue
Guanjun Zhang (2019)
Technical Note: Cortical thickness and density estimation from clinical CT using a prior thickness-density relationship.
Ludovic Humbert (2016)
Effects of dose reduction on bone strength prediction using finite element analysis
D. Anitha (2016)
Multidetector Computed Tomography Imaging: Effect of Sparse Sampling and Iterative Reconstruction on Trabecular Bone Microstructure
M. Mookiah (2018)
Effect of the intervertebral disc on vertebral bone strength prediction: a Finite-Element Study.
D. Anitha (2019)
Semantic Scholar Logo Some data provided by SemanticScholar