Online citations, reference lists, and bibliographies.
← Back to Search

Antibiotic Additive And Synergistic Action Of Rutin, Morin And Quercetin Against Methicillin Resistant Staphylococcus Aureus

M. Amin, M. Khurram, B. Khattak, Jafar Khan
Published 2015 · Medicine

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
BackgroundTo determine the effect of flavonoids in conjunction with antibiotics in methicillin resistant Staphylococcus aureus (MRSA) a study was designed. The flavonoids included Rutin, Morin, Qurecetin while antibiotics included ampicillin, amoxicillin, cefixime, ceftriaxone, vancomycin, methicillin, cephradine, erythromycin, imipenem, sulphamethoxazole/trimethoprim, ciprofloxacin and levolfloxacin. Test antibiotics were mostly found resistant with only Imipenem and Erythromycin found to be sensitive against 100 MRSA clinical isolates and S. aureus (ATCC 43300). The flavonoids were tested alone and also in different combinations with selected antibiotics.MethodsAntibiotics and flavonoids sensitivity assays were carried using disk diffusion method. The combinations found to be effective were sifted through MIC assays by broth macro dilution method. Exact MICs were determined using an incremental increase approach. Fractional inhibitory concentration indices (FICI) were determined to evaluate relationship between antibiotics and flavonoids is synergistic or additive. Potassium release was measured to determine the effect of antibiotic-flavonoids combinations on the cytoplasmic membrane of test bacteria.ResultsAntibiotic and flavonoids screening assays indicated activity of flavanoids against test bacteria. The inhibitory zones increased when test flavonoids were combined with antibiotics facing resistance. MICs of test antibiotics and flavonoids reduced when they were combined. Quercetin was the most effective flavonoid (MIC 260 μg/ml) while morin + rutin + quercetin combination proved most efficient with MIC of 280 + 280 + 140 μg/ml. Quercetin + morin + rutin with amoxicillin, ampicillin, cephradine, ceftriaxone, imipenem, and methicillin showed synergism, while additive relationship was indicated between morin + rutin and amoxicillin, cephradine, ceftriaxone, imipenem, and methicillin. Quercetin alone had an additive effect with ampicillin, cephradine, ceftriaxone, imipenem, and methicillin. Potassium leakage was highest for morin + rutin + quercetin that improved further in combination with imipenem. Morin and rutin alone had no activity but in combination showed activity against test bacteria.ConclusionsThe flavonoids when used in combination with antibiotics were found to increase each other activity against test bacteria. The relationship between the flavonoids and antibiotics in most of the cases was additive. However in a few cases synergism was also observed. Flavonoids alone or in combinations also damaged bacterial cell membrane.
This paper references
10.2307/3758354
Manual of clinical microbiology
P. R. Murray (1975)
10.1128/AAC.42.9.2365
In Vitro Pharmacodynamic Studies of L-749,345 in Comparison with Imipenem and Ceftriaxone against Gram-Positive and Gram-Negative Bacteria
I. Odenholt (1998)
10.1016/S0168-1605(00)00218-X
Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds.
J. Rauha (2000)
Terminology relating to methods for the determination of susceptibility of bacteria to antimicrobial agents. EUCAST Definitive Document E. Def 1.2
(2000)
European Committee on Antimicrobial Susceptibility Testing. Terminology relating to methods for the determination of susceptibility of bacteria to antimicrobial agents
(2000)
Terminology relating to methods for the determination of susceptibility of bacteria to antimicrobial agents . EUCAST Definitive Document E . Def 1 . 2
A Khan (2000)
10.1002/1099-1573(200102)15:1<39::AID-PTR684>3.0.CO;2-R
Activity of plant flavonoids against antibiotic‐resistant bacteria
H. Xu (2001)
10.1271/bbb.66.1009
Rutin-enhanced Antibacterial Activities of Flavonoids against Bacillus cereus and Salmonella enteritidis
Hidetoshi Arima (2002)
10.1086/502213
SHEA Guideline for Preventing Nosocomial Transmission of Multidrug-Resistant Strains of Staphylococcus aureus and Enterococcus
C. Muto (2003)
Micrococcus, and other catalase-positive cocci that grow aerobically Manual of clinical microbiology
Tl Bannerman (2003)
Natural flavonoids as antimicrobial agents
W Bylka (2004)
Antibacterial activity of morin and its complexes with La(III), Gd(III) and Lu(III) ions.
M. Kopacz (2005)
10.1128/AAC.49.2.549-555.2005
Alkyl Gallates, Intensifiers of β-Lactam Susceptibility in Methicillin-Resistant Staphylococcus aureus
H. Shibata (2005)
10.1016/J.JEP.2005.04.014
Detection of galangin-induced cytoplasmic membrane damage in Staphylococcus aureus by measuring potassium loss.
T. P. T. Cushnie (2005)
10.1016/j.ijantimicag.2005.09.002
Antimicrobial activity of flavonoids
Tim Cushnie (2005)
10.32604/biocell.2006.30.039
Synergism of flavonoids with bacteriostatic action against Staphylococcus aureus ATCC 25 923 and Escherichia coli ATCC 25 922.
M. D. L. A. Alvarez (2006)
10.1128/9781683671077.ch1
Staphylococcus, Micrococcus, and Other Catalase-Positive Cocci
T. Bannerman (2006)
Antimicrobial and antifungal activity of Heracleum sphondylium subsp. artivinense
A Ergene (2006)
10.1590/S0001-37652008000300004
Identification of the antimicrobial substances produced by Solanum palinacanthum (Solanaceae).
Aline C Pereira (2008)
10.1007/s12223-008-0003-4
Antimicrobial activity and synergism of some substituted flavonoids
M. Álvarez (2008)
Laboratory diagnosis of infectious diseases : essentials of diagnostic microbiology
Paul G. Engelkirk (2008)
10.1111/J.1469-0691.2007.01935.X
EUCAST Definitive Document EDef 7.1: method for the determination of broth dilution MICs of antifungal agents for fermentative yeasts: Subcommittee on Antifungal Susceptibility Testing (AFST) of the ESCMID European Committee for Antimicrobial Susceptibility Testing (EUCAST)∗
J. L. Rodríguez-Tudela (2008)
Essential of Diagnostic Microbiology In: Gram positive Cocci
Pg Engelkirk (2008)
10.1016/j.ijfoodmicro.2009.07.031
Cell membrane damage induced by phenolic acids on wine lactic acid bacteria.
F. Campos (2009)
Antimicrobial resistance profile of methicillin resistant staphylococcal aureus from skin and soft tissue isolates.
Faiza Idrees (2009)
In vitro combinations of antibiotics and phytochemicals against Pseudomonas aeruginosa.
M. Sakharkar (2009)
A Study on Combinatorial Effects of Various Flavonoids for Their Antibacterial Potential Against Clinically Significant Bacterial Species
Pankaj Goyal (2010)
10.1111/J.1365-2621.2010.02267.X
Characterisation of anti-Staphylococcus aureus activity of quercetin
I. Hirai (2010)
The continuing threat of methicillin-resistant Staphylococcus aureus- Past Present Future
Zaeema Khan (2010)
Physico - chemical and antimicrobial properties of novel rutin derivatives with 6-aminopenicillanic acid
D Lupaşcus (2010)
10.5897/AJMR11.471
Prevention of emergence of fusidic acid and rifampicin resistance in Staphylococcus aureus using phytochemicals
Bhone Myint Kyaw (2011)
Role of secondary metabolites in defense mechanisms of plants
M. Mazid (2011)
10.1002/CHIN.201105264
Secondary Metabolites of Plants from the Genus Saussurea: Chemistry and Biological Activity
Yufang Wang (2011)
10.3390/ijms12063422
Flavonoid Analyses and Antimicrobial Activity of Various Parts of Phaleria macrocarpa (Scheff.) Boerl Fruit
R. Hendra (2011)
10.3923/MJ.2011.89.96
Synergism and Postantibiotic Effect of Green Tea Extract and Imipenem Against Methicillin-resistant Staphylococcus aureus
E. Aboulmagd (2011)
10.1093/jac/dkr101
Comparative ceftaroline activity tested against pathogens associated with community-acquired pneumonia: results from an international surveillance study.
R. Jones (2011)
10.1002/cbdv.201100172
Secondary Metabolites of Plants from the Genus Cipadessa: Chemistry and Biological Activity
A. K. Bandi (2012)
10.1155/2012/202608
In vitro assessment of antibacterial activity and cytocompatibility of quercetin-containing PLGA nanofibrous scaffolds for tissue engineering
Zhi-Cai Xing (2012)
10.1590/S1517-838220120003000013
Bactericidal antibiotic-phytochemical combinations against methicillin resistant Staphylococcus aureus
Bhone Myint Kyaw (2012)
JeongY KyuKang I. In vitro assessment of antibacterial activity and cytocompatibility of quercetin-containing PLGA nanofibrous scaffolds for tissue engineering
ZC Xing (2012)
Comparative study on the antibacterial activity of some flavonoids and their sulfonic derivatives.
E. Woźnicka (2013)



This paper is referenced by
10.3390/plants10081659
Antioxidant Activity, α-Glucosidase Inhibition and UHPLC–ESI–MS/MS Profile of Shmar (Arbutus pavarii Pamp)
Nawal Buzgaia (2021)
10.3389/fcimb.2021.709749
Fighting the Antibiotic Crisis: Flavonoids as Promising Antibacterial Drugs Against Helicobacter pylori Infection
Andrés González (2021)
10.3389/fmicb.2021.556550
Quinoline Antimalarials Increase the Antibacterial Activity of Ampicillin
Olajumoke A. Olateju (2021)
10.1016/J.TIFS.2021.03.030
Food flavonols: Nutraceuticals with complex health benefits and functionalities
D. Barreca (2021)
10.3390/molecules26134021
Dietary Flavonoids: Cardioprotective Potential with Antioxidant Effects and Their Pharmacokinetic, Toxicological and Therapeutic Concerns
Johra Khan (2021)
10.3390/molecules26123634
Isoflavonoid-Antibiotic Thin Films Fabricated by MAPLE with Improved Resistance to Microbial Colonization
V. Grumezescu (2021)
10.1101/2020.03.08.983049
Naringenin as an antibacterial reagent controlling of biofilm formation and fatty acid metabolism in MRSA
Hun-Suk Song (2020)
10.36295/asro.2020.231215
INHIBITION EFFECT OF GREEN APPLE PEEL EXTRACTS ON SOME TYPES OF PATHOGENIC BACTERIA ISOLATED FROM VAGINA
Bilal Salim Dawood Al-Taie (2020)
10.1007/s42770-020-00284-3
Synergistic effects of pomegranate and rosemary extracts in combination with antibiotics against antibiotic resistance and biofilm formation of Pseudomonas aeruginosa.
Wael Mohamed Abu El-Wafa (2020)
10.1039/d0fo01444c
Morin as an imminent functional food ingredient: an update on its enhanced efficacy in the treatment and prevention of metabolic syndromes.
K. Thakur (2020)
10.22207/jpam.14.4.44
Antibiotics Susceptibility Profile and Synergistic Effects of Flavonoids with Antibiotics against Resistant Staphylococcus aureus from Asymptomatic Individuals
Rudra Mishra (2020)
10.1007/s00284-020-02125-w
Phytochemical Characterization, Bioactivities Evaluation and Synergistic Effect of Arbutus unedo and Crataegus monogyna Extracts with Amphotericin B
Alexandra T. Coimbra (2020)
10.1038/s41598-020-74977-y
Quercetin inhibits virulence properties of Porphyromas gingivalis in periodontal disease
Zhiyan He (2020)
10.1186/s11671-020-03297-x
Designing Aptamer-Gold Nanoparticle-Loaded pH-Sensitive Liposomes Encapsulate Morin for Treating Cancer
X. Ding (2020)
10.1016/j.eujim.2020.101061
The antibacterial and antivirulent potential of Hypericum lydium against Staphylococcus aureus: Inhibition of growth, biofilm formation, and hemolytic activity
Abdurrahman Aygül (2020)
10.1101/2020.09.01.20186353
Synergistic effects of natural products and commercial antibiotics
L. Nitsch-Velasquez (2020)
10.1016/j.micpath.2020.104120
Demonstration of bactericidal and synergistic activity of quercetin with meropenem among pathogenic carbapenem resistant Escherichia coli and Klebsiella pneumoniae.
Arijit Pal (2020)
10.1016/j.ijfoodmicro.2020.108539
Evaluation of antimicrobial activity of rambutan (Nephelium lappaceum L.) peel extracts.
N. Phương (2020)
10.3390/foods9081132
Japanese Quince (Chaenomeles japonica) as a Potential Source of Phenols: Optimization of the Extraction Parameters and Assessment of Antiradical and Antimicrobial Activities
I. Urbanavičiūtė (2020)
10.1016/j.fitote.2020.104720
Antimicrobial flavonoids as a potential substitute for overcoming antimicrobial resistance.
Avadh Biharee (2020)
10.1007/s11274-020-02934-y
Effect of resveratrol and quercetin on the susceptibility of Escherichia coli to antibiotics.
O. Oktyabrsky (2020)
10.1371/journal.pone.0236319
GC-MS analysis, antimicrobial, antioxidant, antilipoxygenase and cytotoxic activities of Jacaranda mimosifolia methanol leaf extracts and fractions
R. Naz (2020)
10.3390/pr8040446
Saudi Rosmarinus officinalis and Ocimum basilicum L. Polyphenols and Biological Activities
H. Elansary (2020)
10.1016/j.sjbs.2020.09.016
Propolis as a novel antibacterial agent
M. Almuhayawi (2020)
10.4028/www.scientific.net/KEM.840.205
Antibacterial Activity of Traditional Medicine Scurrula atropurpurea (BL) DANS and their Endophytic Fungi
Elfita Elfita (2020)
10.3390/pr8020138
Antiproliferative, Antimicrobial, and Antifungal Activities of Polyphenol Extracts from Ferocactus Species
Hosam O. Elansary (2020)
10.3390/app10041519
Antioxidant, Antimicrobial, Antidiabetic and Cytotoxic Activity of Crocus sativus L. Petals
A. Wali (2020)
10.1371/journal.pone.0241304
Potential synergistic activity of quercetin with antibiotics against multidrug-resistant clinical strains of Pseudomonas aeruginosa
C. Vipin (2020)
10.1016/j.sajce.2020.09.007
Antibacterial effects of Musa sp. ethanolic leaf extracts against methicillin-resistant and susceptible Staphylococcus aureus
L. A. Sivasamugham (2020)
10.1155/2020/3903682
Antimicrobial, Cytotoxic, and Antioxidant Potential of a Novel Flavone “6,7,4′-Trimethyl Flavone” Isolated from Wulfenia amherstiana
M. Kakar (2020)
10.1038/s41598-020-71025-7
Strain-specific anti-biofilm and antibiotic-potentiating activity of 3′,4′-difluoroquercetin
Wonyoung Kho (2020)
10.1016/J.INDCROP.2019.111474
Natural variability and antioxidant properties of commercially cultivated switchgrass extractives
Jingming Tao (2019)
See more
Semantic Scholar Logo Some data provided by SemanticScholar