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Production Of A Microcapsule Agent Of Chromate-reducing Lysinibacillus Fusiformis ZC1 And Its Application In Remediation Of Chromate-spiked Soil

Jun Huang, J. Li, G. Wang
Published 2016 · Chemistry, Medicine

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Lysinibacillus fusiformis ZC1 is an efficient Cr(VI)-reducing bacterium that can transform the toxic and soluble chromate [Cr(VI)] form to the less toxic and precipitated chromite form [Cr(III)]. As such, this strain might be applicable for bioremediation of Cr(VI) in soil by reducing its bioavailability. The study objective was to prepare a microcapsule agent of strain ZC1 for bioremediation of Cr(VI)-contaminated soil. Using a single-factor orthogonal array design, the optimal fermentation medium was obtained and consisted of 6 g/L corn flour, 12 g/L soybean flour, 8 g/L NH4Cl and 6 g/L CaCl2. After enlarged fermentation, the cell and spore densities were 5.9 × 109 and 1.7 × 108 cfu/mL, respectively. The fermentation products were collected and embedded with 1 % gum arabic and 1 % sorbitol as the microcapsule carriers and were subsequently spray-dried. Strain ZC1 exhibited viable cell counts of (3.6 ± 0.44) × 1010 cfu/g dw after 50-day storage at room temperature. In simulated soil bioremediation experiments, 67 % of Cr(VI) was reduced in 5 days with the inoculation of this microcapsule agent, and the Cr(VI) concentration was below the soil Cr(VI) standard level. The results demonstrated that the microcapsule agent of strain ZC1 is efficient for bioremediation of Cr(VI)-contaminated soil.
This paper references
Experimental Designs, 2nd Edition
W. G. Cochran (1950)
10.1007/s00253-008-1768-8
Bioindicator production with Bacillus atrophaeus’ thermal-resistant spores cultivated by solid-state fermentation
S. R. B. R. Sella (2009)
10.1016/j.ecoenv.2015.02.036
Versatility of Streptomyces sp. M7 to bioremediate soils co-contaminated with Cr(VI) and lindane.
JuanDaniel Aparicio (2015)
10.1007/s00253-009-2162-x
Greater enhancement of Bacillus subtilis spore yields in submerged cultures by optimization of medium composition through statistical experimental designs
Zhen-Min Chen (2009)
10.1016/J.FOODRES.2013.05.024
Impact of heat treatment and spray drying on cellular properties and culturability of Bifidobacterium bifidum BB-12
S. Salar-Behzadi (2013)
10.2113/GSELEMENTS.8.2.107
Remediation of Chromium and Uranium Contamination by Microbial Activity
Y. Cheng (2012)
10.2134/jeq2012.0061
Reduction kinetics of hexavalent chromium in soils and its correlation with soil properties.
Wendan Xiao (2012)
10.1007/s10529-007-9436-0
Culture and spray-drying of Tsukamurella paurometabola C-924: stability of formulated powders
A. Hernández (2007)
10.1016/J.FOODRES.2011.03.053
Towards a maximal cell survival in convective thermal drying processes
N. Fu (2011)
Formulations of viable microorganisms and their methods of production and use
GE Harman (2006)
10.1007/S11274-009-0047-X
Removal of toxic chromate using free and immobilized Cr(VI)-reducing bacterial cells of Intrasporangium sp. Q5-1
J. Yang (2009)
10.1007/s10534-007-9121-8
Mechanisms of bacterial resistance to chromium compounds
M. I. Ramírez-Díaz (2007)
10.1016/S0300-483X(02)00378-5
Cytotoxicity and oxidative mechanisms of different forms of chromium.
D. Bagchi (2002)
10.1080/01490451.2014.971200
Chromate Interaction with the Chromate Reducing Actinobacterium Intrasporangium chromatireducens Q5-1
H. Liu (2015)
10.1016/S0168-1605(01)00733-4
Survival of bifidobacteria after spray-drying.
Wen-Chian Lian (2002)
10.1128/AEM.68.8.3859-3866.2002
Association of Microbial Community Composition and Activity with Lead, Chromium, and Hydrocarbon Contamination
W. Shi (2002)
10.1016/J.FBP.2013.01.005
Comparison of cell survival rates of E. coli K12 and L. acidophilus undergoing spray drying
S. Pispan (2013)
10.1016/j.jenvman.2015.04.011
Bioremediation of hexavalent chromate using permeabilized Brevibacterium sp. and Stenotrophomonas sp. cells.
Shimei Ge (2015)
10.1016/S0045-6535(02)00089-9
Chromium (VI) biosorption and bioaccumulation by chromate resistant bacteria.
T. Srinath (2002)
10.1007/S11947-008-0135-1
Resistance of Microencapsulated Lactobacillus acidophilus LA1 to Processing Treatments and Simulated Gut Conditions
L. Sabikhi (2010)
Formulations of viable microorganisms and their methods of production and use. US Patent Application Number
G E Harman (2006)
Formulations of viable microorganisms and their methods of production and use. US Patent Application Number: PCT/ US2006/034744
GE Harman (2006)
10.1021/es203101f
Chromium contamination accident in China: viewing environment policy of China.
Yang Gao (2011)
Bioremediation of chromium contaminated soil by Pseudomonas fluorescens and indigenous microorganisms.
D. Jeyalakshmi (2008)
10.1016/j.jhazmat.2013.01.048
Chemical and microbial remediation of hexavalent chromium from contaminated soil and mining/metallurgical solid waste: a review.
B. Dhal (2013)
10.1007/s10646-014-1294-7
Global transcriptome analysis of hexavalent chromium stress responses in Staphylococcus aureus LZ-01
X. Zhang (2014)
10.1016/S0039-9140(02)00317-X
Determination of total chromium traces in tannery effluents by electrothermal atomic absorption spectrometry, flame atomic absorption spectrometry and UV-visible spectrophotometric methods.
M. I. C. Monteiro (2002)
10.1016/J.BIOCONTROL.2010.11.008
Microencapsulating aerial conidia of Trichoderma harzianum through spray drying at elevated temperatures
X. Jin (2011)
The metals translator: guidance for converting a total recoverable permit limit from a dissolved criterion
(1996)
10.1002/jobm.200800239
Bioremediation of chromium(VI) contaminated soil by Streptomyces sp. MC1
M. A. Polti (2009)
10.1007/s10526-006-9055-z
Application of statistically-based experimental designs in medium optimization for spore production of Bacillus subtilis from distillery effluent
Fengyu Shi (2006)
10.1016/J.WASMAN.2006.12.012
Stabilization of As, Cr, Cu, Pb and Zn in soil using amendments--a review.
J. Kumpiene (2008)
10.2134/jeq2013.07.0267
Chromium-resistant bacteria promote the reduction of hexavalent chromium in soils.
Wendan Xiao (2014)
10.1016/J.APSOIL.2014.09.002
Bacterial community dynamics during bioremediation of Cr(VI)-contaminated soil
Yangyang Wang (2015)
10.1111/J.1462-2920.2004.00639.X
Mechanism of chromate reduction by the Escherichia coli protein, NfsA, and the role of different chromate reductases in minimizing oxidative stress during chromate reduction.
D. Ackerley (2004)
10.1081/DRT-200063478
Recent Developments in Microencapsulation of Food Ingredients
K. G. Desai (2005)
10.1016/j.jhazmat.2010.09.072
Characterization and genomic analysis of a highly chromate resistant and reducing bacterial strain Lysinibacillus fusiformis ZC1.
Minyan He (2011)
10.1016/J.ECOLENG.2014.09.093
Effect of bacterial treatment on Cr(VI) remediation from soil and subsequent plantation of Pisum sativum
D. Kumari (2014)
10.1016/J.IDAIRYJ.2008.10.008
The protective effect of monosodium glutamate on survival of Lactobacillus rhamnosus GG and Lactobacillus rhamnosus E-97800 (E800) strains during spray-drying and storage in trehalose-containing powders
E. O. Sunny‐Roberts (2009)
10.1016/J.EJSOBI.2013.02.002
Chromium reducing and plant growth promoting novel strain Pseudomonas aeruginosa OSG41 enhance chickpea growth in chromium amended soils
M. Oves (2013)
10.1016/J.MIMET.2006.02.017
Preservation of micro-organisms by drying; a review.
C. Morgan (2006)
10.1016/j.jhazmat.2009.01.030
Cr (VI) remediation by indigenous bacteria in soils contaminated by chromium-containing slag.
Li-yuan Chai (2009)
10.1016/J.CEJ.2011.02.060
Ex situ bioremediation of Cr(VI) contaminated soil by Bacillus sp.: Batch and continuous studies
Mathur Nadarajan Kathiravan (2011)
10.1111/j.1365-2672.2012.05257.x
Reutilization of immobilized fungus Rhizopus sp. LG04 to reduce toxic chromate
H. Liu (2012)
10.1016/j.resmic.2008.10.003
Tracking the influence of long-term chromium pollution on soil bacterial community structures by comparative analyses of 16S rRNA gene phylotypes.
Chirayu Desai (2009)
10.1016/J.SEPPUR.2012.07.010
Electrokinetic remediation of chromium- and cadmium-contaminated soil from abandoned industrial site
Ping Lu (2012)
10.1016/J.PROCBIO.2006.10.007
Medium optimization of carbon and nitrogen sources for the production of spores from Bacillus amyloliquefaciens B128 using response surface methodology
Y. Rao (2007)
10.1016/S0032-9592(97)00031-9
Medium improvement by orthogonal array designs for cholesterol oxidase production by Rhodococcus equi No. 23
Ming‐Tsung Lee (1997)
Formulations of viable microorganisms and their methods of production and use. US Patent Application Number: PCT/ US2006
Ge Harman (2006)
10.1016/j.cbi.2010.04.018
Chromium genotoxicity: A double-edged sword.
Kristen P. Nickens (2010)
10.1016/j.ecoenv.2014.07.007
Isolating, screening and applying chromium reducing bacteria to promote growth and yield of okra (Hibiscus esculentus L.) in chromium contaminated soils.
Zahid Maqbool (2015)
10.1128/AEM.66.6.2605-2612.2000
Comparative Survival Rates of Human-Derived ProbioticLactobacillus paracasei and L. salivariusStrains during Heat Treatment and Spray Drying
G. Gardiner (2000)
10.1016/J.TOX.2006.08.005
Oral administration of Cr(VI) induced oxidative stress, DNA damage and apoptotic cell death in mice.
X. Wang (2006)
10.1021/ES050185F
Treatment of hexavalent chromium in chromite ore processing solid waste using a mixed reductant solution of ferrous sulfate and sodium dithionite.
C. Su (2005)
10.1007/s13762-011-0013-7
Remediation of chromium contaminants using bacteria
P. Kanmani (2011)
10.1186/1471-2180-10-221
Characterization and genomic analysis of chromate resistant and reducing Bacillus cereus strain SJ1
Minyan He (2010)
10.2174/0929867053764635
Metals, toxicity and oxidative stress.
M. Valko (2005)
10.1007/s11356-013-2098-7
A Cr(VI)-reducing Microbacterium sp. strain SUCR140 enhances growth and yield of Zea mays in Cr(VI) amended soil through reduced chromium toxicity and improves colonization of arbuscular mycorrhizal fungi
S. Soni (2013)
10.1002/widm.1046
Experimental design
J. P. Morgan (2012)
10.1007/s11356-013-1919-z
Physicochemical and biological quality of soil in hexavalent chromium-contaminated soils as affected by chemical and microbial remediation
Yingping Liao (2013)
10.1007/s10532-006-9096-0
Evaluation of biosorption potency of Acinetobacter sp. for removal of hexavalent chromium from tannery effluent
S. Srivastava (2006)
Chromium genotoxicity: a doubleedged sword
KP Nickens (2010)
10.1007/BF03392533
Experimental Designs
A. Baron (1990)
10.1016/j.jhazmat.2009.09.113
A critical review on the bio-removal of hazardous heavy metals from contaminated soils: issues, progress, eco-environmental concerns and opportunities.
Gang Wu (2010)



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