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Caveolin-1 Does Not Affect SR-BI-mediated Cholesterol Efflux Or Selective Uptake Of Cholesteryl Ester In Two Cell Lines Published, JLR Papers In Press, January 16, 2003. DOI 10.1194/jlr.M200449-JLR200
Libo Wang, M. Connelly, A. G. Ostermeyer, Hsu-hsin Chen, D. Williams, D. A. Brown
Published 2003 · Medicine, Chemistry
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Free cholesterol (FC) has been reported to efflux from cells through caveolae, which are 50–100 nm plasma membrane pits. The 22 kDa protein caveolin-1 is concentrated in caveolae and is required for their formation. The HDL scavenger receptor BI (SR-BI), which stimulates both FC efflux and selective uptake of HDL-derived cholesteryl ester (CE), has been reported to be concentrated in caveolae, suggesting that this localization facilitates flux of FC and CE across the membrane. However, we found that overexpression of caveolin-1 in Fischer rat thyroid (FRT) cells, which lack caveolin-1 and caveolae, or HEK 293 cells, which normally express very low levels of caveolin-1, did not affect FC efflux to HDL or liposomes. Transient expression of SR-B1 did not affect this result. Similarly, caveolin-1 expression did not affect selective uptake of CE from labeled HDL particles in FRT or HEK 293 cells transfected with SR-BI. We conclude that basal and SR-BI-stimulated FC efflux to HDL and liposomes and SR-BI-mediated selective uptake of HDL CE are not affected by caveolin-1 expression in HEK 293 or FRT cells.
This paper references
ABCA1. The gatekeeper for eliminating excess tissue cholesterol.
J. Oram (2001)
Caveolin Is Palmitoylated on Multiple Cysteine Residues
D. Dietzen (1995)
Decreased Atherosclerosis in Heterozygous Low Density Lipoprotein Receptor-deficient Mice Expressing the Scavenger Receptor BI Transgene*
T. Arai (1999)
Membrane microdomains and caveolae.
T. Kurzchalia (1999)
Transport of Lipids from High and Low Density Lipoproteins via Scavenger Receptor-BI*
H. Stangl (1999)
Charting the fate of the "good cholesterol": identification and characterization of the high-density lipoprotein receptor SR-BI.
M. Krieger (1999)
Analysis of chimeric receptors shows that multiple distinct functional activities of scavenger receptor, class B, type I (SR-BI), are localized to the extracellular receptor domain.
M. Connelly (2001)
The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum.
R. Havel (1955)
Simultaneous induction of an HDL receptor protein (SR-BI) and the selective uptake of HDL-cholesteryl esters in a physiologically relevant steroidogenic cell model.
S. Azhar (1998)
Plasma membrane caveolae mediate the efflux of cellular free cholesterol.
P. Fielding (1995)
Murine SR-BI, a High Density Lipoprotein Receptor That Mediates Selective Lipid Uptake, Is N-Glycosylated and Fatty Acylated and Colocalizes with Plasma Membrane Caveolae*
J. Babitt (1997)
Adenovirus-mediated expression of caveolin-1 in mouse liver increases plasma high-density lipoprotein levels.
P. Frank (2001)
De novo formation of caveolae in lymphocytes by expression of VIP21-caveolin.
A. Fra (1995)
Caveolin, a protein component of caveolae membrane coats
K. G. Rothberg (1992)
Regulated Intramembrane Proteolysis A Control Mechanism Conserved from Bacteria to Humans
M. Brown (2000)
Structure and Function of Sphingolipid- and Cholesterol-rich Membrane Rafts*
D. Brown (2000)
Caveolin Isoforms Differ in Their N-terminal Protein Sequence and Subcellular Distribution. IDENTIFICATION AND EPITOPE MAPPING OF AN ISOFORM-SPECIFIC MONOCLONAL ANTIBODY PROBE (*)
P. Scherer (1995)
Scavenger Receptor BI Promotes High Density Lipoprotein-mediated Cellular Cholesterol Efflux*
Y. Ji (1997)
Reverse cholesterol transport.
G. Rothblat (1986)
VIP21/caveolin is a cholesterol-binding protein.
M. Murata (1995)
Structure and Origin of Ordered Lipid Domains in Biological Membranes
D. Brown (1998)
Binding and Cross-linking Studies Show That Scavenger Receptor BI Interacts with Multiple Sites in Apolipoprotein A-I and Identify the Class A Amphipathic α-Helix as a Recognition Motif*
D. Williams (2000)
Stabilization of caveolin-1 by cellular cholesterol and scavenger receptor class B type I.
P. Frank (2002)
Caveolins, Liquid-Ordered Domains, and Signal Transduction
E. Smart (1999)
Expression of Caveolin-1 Is Required for the Transport of Caveolin-2 to the Plasma Membrane
I. Parolini (1999)
Caveolin-1 negatively regulates SR-BI mediated selective uptake of high-density lipoprotein-derived cholesteryl ester.
S. Matveev (2001)
Intracellular cholesterol transport in synchronized human skin fibroblasts.
C. Fielding (1999)
Caveolin Transfection Results in Caveolae Formation but Not Apical Sorting of Glycosylphosphatidylinositol (GPI)-anchored Proteins in Epithelial Cells
C. Lipardi (1998)
Glycosphingolipids Are Not Essential for Formation of Detergent-resistant Membrane Rafts in Melanoma Cells
A. G. Ostermeyer (1999)
Influence of caveolin-1 on cellular cholesterol efflux mediated by high-density lipoproteins.
P. Frank (2001)
Morphological and functional polarity of an epithelial thyroid cell line.
L. Nitsch (1985)
Scavenger receptor BI and cholesterol trafficking.
D. Williams (1999)
A Role for Caveolin and the Urokinase Receptor in Integrin-mediated Adhesion and Signaling
Y. Wei (1999)
Identification of Scavenger Receptor SR-BI as a High Density Lipoprotein Receptor
S. Acton (1996)
Caveolin-1, a protein component of caveolae membrane
K. G. Rothberg (1992)
Influence of the high density lipoprotein receptor SR-BI on reproductive and cardiovascular pathophysiology.
B. Trigatti (1999)
Characterization of proteins in detergent-resistant membrane complexes from Madin-Darby canine kidney epithelial cells.
K. Melkonian (1995)
The Class B, Type I Scavenger Receptor Promotes the Selective Uptake of High Density Lipoprotein Cholesterol Ethers into Caveolae*
G. Graf (1999)
A Caveolin Dominant Negative Mutant Associates with Lipid Bodies and Induces Intracellular Cholesterol Imbalance
Albert Pol (2001)
Protein measurement with the Folin phenol reagent.
O. H. Lowry (1951)
Comparison of Class B Scavenger Receptors, CD36 and Scavenger Receptor BI (SR-BI), Shows That Both Receptors Mediate High Density Lipoprotein-Cholesteryl Ester Selective Uptake but SR-BI Exhibits a Unique Enhancement of Cholesteryl Ester Uptake*
M. Connelly (1999)
Overexpression of the HDL receptor SR-BI alters plasma HDL and bile cholesterol levels
K. Kozarsky (1997)
Hepatic Scavenger Receptor BI Promotes Rapid Clearance of High Density Lipoprotein Free Cholesterol and Its Transport into Bile*
Y. Ji (1999)
Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface
D. Brown (1992)
Utilization of cholesterol-rich lipoproteins by perfused rat adrenals.
S. Azhar (1989)
Involvement of caveolin-1 in cholesterol enrichment of high density lipoprotein during its assembly by apolipoprotein and THP-1 cells.
R. Arakawa (2000)
Scavenger receptor class B, type I (SR-BI) is the major route for the delivery of high density lipoprotein cholesterol to the steroidogenic pathway in cultured mouse adrenocortical cells.
R. Temel (1997)
Dissociation of tissue uptake of cholesterol ester from that of apoprotein A-I of rat plasma high density lipoprotein: selective delivery of cholesterol ester to liver, adrenal, and gonad.
C. Glass (1983)
Upregulation of selective cholesteryl ester uptake pathway in mice with deletion of low‐density lipoprotein receptor function
S. Azhar (1999)
Caveolin-1-deficient Mice Are Lean, Resistant to Diet-induced Obesity, and Show Hypertriglyceridemia with Adipocyte Abnormalities*
B. Razani (2002)
[Scavenger receptor class B type I (SR-BI)].
Y. Ueda (2001)
Expression of scavenger receptor BI in COS-7 cells alters cholesterol content and distribution.
G. Kellner-Weibel (2000)
Replacement of the cytoplasmic domain alters sorting of a viral glycoprotein in polarized cells.
L. Puddington (1987)
Scavenger receptor BI (SR-BI) mediates free cholesterol flux independently of HDL tethering to the cell surface.
M. de la Llera-Moya (1999)
Expression of human lecithin:cholesterol acyltransferase in transgenic mice: effects on cholesterol efflux, esterification, and transport.
O. Francone (1997)
Cell cholesterol efflux: integration of old and new observations provides new insights.
G. Rothblat (1999)
Functional rafts in cell membranes
Kai Simons (1997)
Scavenger Receptor Class B Type I as a Mediator of Cellular Cholesterol Efflux to Lipoproteins and Phospholipid Acceptors*
B. Jian (1998)
This paper is referenced by
Cell-based screening assay for anti-inflammatory activity of bioactive compounds.
Kees Meijer (2015)
Voltage-dependent capacitance of human embryonic kidney cells.
B. Farrell (2006)
Caveolin-1 alters Ca2+ signal duration through specific interaction with the Gαq family of G proteins
Parijat Sengupta (2008)
Rupture of the Atherosclerotic Plaque: Does a Good Animal Model Exist?
P. Cullen (2003)
Expression of caveolin‐1 in hepatic cells increases oxidized LDL uptake and preserves the expression of lipoprotein receptors
T. Q. Truong (2009)
Transport of cholesterol across a BeWo cell monolayer: implications for net transport of sterol from maternal to fetal circulation.
Kara E. Schmid (2003)
Directed evolution and biophysical characterization of a full-length, soluble, human caveolin-1 variant.
J. N. Smith (2018)
Rupture of the atherosclerotic plaque : does a good animal model exist ?
von Eckardstein (2003)
Caveolin as a Novel Potential Therapeutic Target in Cardiac and Vascular Diseases: A Mini Review
Jinfan Tian (2020)
Opposite effect of caveolin-1 in the metabolism of high-density and low-density lipoproteins.
T. Q. Truong (2006)
Cellular cholesterol delivery, intracellular processing and utilization for biosynthesis of steroid hormones
J. Hu (2010)
Regulation der Expression von Scavenger Receptor BI (SR-BI) und des bidirektionalen Cholesterolflux durch den Cholesterol- und Vitamin-E-Gehalt in HepG2-Zellen und High Density Lipoproteinen
P. Barikbin (2004)
High-density lipoprotein metabolism and the human embryo.
V. Fujimoto (2010)
Scavenger receptor class B member 1 protein: hepatic regulation and its effects on lipids, reverse cholesterol transport, and atherosclerosis
Anthony P Kent (2011)
SR‐BI, CD36, and caveolin‐1 contribute positively to cholesterol efflux in hepatic cells
T. Q. Truong (2010)
ENDOGENOUS AND EXOGENOUS SOURCES OF CHOLESTEROL DURING FETAL DEVELOPMENT
Kara E. Schmid (2003)
Caveolin-1 interacts with the Gag precursor of murine leukaemia virus and modulates virus production
Zheng Yu (2006)
Roles of ATP binding cassette transporters A1 and G1, scavenger receptor BI and membrane lipid domains in cholesterol export from macrophages
W. Jessup (2006)
Prolonged sleep restriction induces changes in pathways involved in cholesterol metabolism and inflammatory responses
Vilma Aho (2016)
Ontogeny, immunolocalisation, distribution and function of SR-BI in the human intestine
E. Levy (2004)
Expression of Caveolin-1 Enhances Cholesterol Efflux in Hepatic Cells*
Y. Fu (2004)
Gene profiling of cathepsin K deficiency in atherogenesis: profibrotic but lipogenic
S. Lutgens (2006)
Interleukin-1-induced gene expression requires the membrane-raft-dependent internalization of the interleukin-1 receptor.
Mark Windheim (2016)
Caveolin-1 and regulation of cellular cholesterol homeostasis.
P. Frank (2006)
Sterol carrier protein-2: new roles in regulating lipid rafts and signaling.
F. Schroeder (2007)
Scavenger receptor BI (SR-BI) clustered on microvillar extensions suggests that this plasma membrane domain is a way station for cholesterol trafficking between cells and high-density lipoprotein.
Yinan Peng (2004)
Influence of the HDL Receptor SR-BI on Lipoprotein Metabolism and Atherosclerosis
B. Trigatti (2003)
Dissociation of Insulin Receptor Expression and Signaling from Caveolin-1 Expression*
J. Wharton (2005)
Scavenger receptor, class B, type I mediates the uptake and degradation of beta-VLDL particles in tissue culture
Stefanie Fruhwürth (2009)
Flotillin-1 is essential for PKC-triggered endocytosis and membrane microdomain localization of DAT
M. L. Cremona (2011)
Role of caveolin-1 in the regulation of lipoprotein metabolism.
P. Frank (2008)
New insight on the molecular mechanisms of high-density lipoprotein cellular interactions
L. O. Martinez (2004)See more