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The Effects Of A Probiotic Yeast On The Bacterial Diversity And Population Structure In The Rumen Of Cattle

E. Pinloche, N. McEwan, J. Marden, C. Bayourthe, E. Auclair, C. J. Newbold
Published 2013 · Biology, Medicine

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It has been suggested that the ability of live yeast to improve milk yield and weight gain in cattle is because the yeast stimulates bacterial activity within the rumen. However it remains unclear if this is a general stimulation of all species or a specific stimulation of certain species. Here we characterised the change in the bacterial population within the rumen of cattle fed supplemental live yeast. Three cannulated lactating cows received a daily ration (24 kg/d) of corn silage (61% of DM), concentrates (30% of DM), dehydrated alfalfa (9% of DM) and a minerals and vitamins mix (1% of DM). The effect of yeast (BIOSAF SC 47, Lesaffre Feed Additives, France; 0.5 or 5 g/d) was compared to a control (no additive) in a 3×3 Latin square design. The variation in the rumen bacterial community between treatments was assessed using Serial Analysis of V1 Ribosomal Sequence Tag (SARST-V1) and 454 pyrosequencing based on analysis of the 16S rRNA gene. Compared to the control diet supplementation of probiotic yeast maintained a healthy fermentation in the rumen of lactating cattle (higher VFA concentration [high yeast dose only], higher rumen pH, and lower Eh and lactate). These improvements were accompanied with a shift in the main fibrolytic group (Fibrobacter and Ruminococcus) and lactate utilising bacteria (Megasphaera and Selenomonas). In addition we have shown that the analysis of short V1 region of 16s rRNA gene (50–60 bp) could give as much phylogenetic information as a longer read (454 pyrosequencing of 250 bp). This study also highlights the difficulty of drawing conclusions on composition and diversity of complex microbiota because of the variation caused by the use of different methods (sequencing technology and/or analysis).
This paper references
Manipulation of ruminal fermentation. IV. Effect of altering ruminal pH on volatile fatty acid production.
W. J. Esdale (1972)
Rumen Bacterial Competition in Continuous Culture: Streptococcus bovis Versus Megasphaera elsdenii
J. Russell (1981)
Toxicity of organic extraction reagents to anaerobic bacteria
M. J. Playne (1983)
Influence of addition of yeast culture supplement to diets of lactating cows on ruminal fermentation and microbial populations.
G. A. Harrison (1988)
Comparison of corn with four sorghum grain hybrids: site and extent of digestion in steers.
M. Streeter (1990)
Effects of Physicochemical Factors on the Adhesion to Cellulose Avicel of the Ruminal Bacteria Ruminococcus flavefaciens and Fibrobacter succinogenes subsp. succinogenes
V. Roger (1990)
Impact of metabolism modifiers on protein accretion and protein and energy requirements of livestock
R. D. Boyd (1991)
Effects of the inclusion of yeast culture (Saccharomyces cerevisiae plus growth medium) in the diet of dairy cows on milk yield and forage degradation and fermentation patterns in the rumen of steers.
P. Williams (1991)
Rumen microbiology, biotechnology and ruminant nutrition: the application of research findings to a complex microbial ecosystem.
R. Wallace (1992)
Effect of yeast culture supplement on production, rumen fermentation, and duodenal nitrogen flow in dairy cows.
L. Erasmus (1992)
Effects of yeast on dairy cow performance, ruminal fermentation, blood components, and milk manufacturing properties.
G. Piva (1993)
Rumen fermentation and its manipulation: the development of yeast cultures as feed additives
R. J. Wallace (1993)
Effect of a yeast culture (Saccharomyces cerevisiae) on neutral detergent fiber digestion in steers fed oat straw based diets
F. Plata (1994)
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
J. Thompson (1994)
Nutrient transport by ruminal bacteria: a review.
S. A. Martin (1994)
Manipulation of microbial activity in the rumen.
J. Jouany (1994)
Mode of action of the yeast Saccharomyces cerevisiae as a feed additive for ruminants.
C. Newbold (1996)
Effects of a strain of Saccharomyces cerevisiae (Levucell SC1), a microbial additive for ruminants, on lactate metabolism in vitro.
F. Chaucheyras (1996)
Optimal blocked main effects plans with nested rows and columns and related designs
N. Uddin (1996)
Bovine acidosis: implications on laminitis.
J. Nocek (1997)
Effects of a Saccharomyces cerevisiae culture on ruminal bacteria that utilize lactate and digest cellulose.
E. Callaway (1997)
The Rumen Microbial Ecosystem, 2nd edition
PN Hobson (1997)
Changes in the microbial population of a rumen-simulating fermenter in response to yeast culture
C. Newbold (1998)
Acidosis in cattle: a review.
F. N. Owens (1998)
Manipulating Ruminal Fermentation : A Microbial Ecological Perspective
U. S. Dairy (1998)
Ruminal microbial and fermentative changes associated with experimentally induced subacute acidosis in steers.
D. Goad (1998)
T. Hall (1999)
Rumen bacterial diversity as determined by sequence analysis of 16S rDNA libraries
K. Tajima (1999)
Rumen Bacterial Community Transition During Adaptation to High-grain Diet
K. Tajima (2000)
Rumen Bacterial Community Transition During Adaptation to Highgrain Diet Anaerobe
K Tajima (2000)
A new method for non-parametric multivariate analysis of variance in ecology
Marti J. Anderson (2001)
The Closest BLAST Hit Is Often Not the Nearest Neighbor
L. Koski (2001)
Stimulation of the Megasphaera elsdeniis butyrate production in continuous culture by a yeast additive
O. Soto-Cruz (2001)
Diet-Dependent Shifts in the Bacterial Population of the Rumen Revealed with Real-Time PCR
K. Tajima (2001)
Dietdependent shifts in the bacterial population of the rumen revealed with real-time PCR
K Tajima (2001)
Mitsuokella jalaludinii sp. nov., from the rumens of cattle in Malaysia.
G. Lan (2002)
The role of dynamic modelling in understanding the microbial contribution to rumen function
J. Dijkstra (2002)
Rumen Microbiology and Its Role in Ruminant Nutrition
J B Russell (2002)
Rumen Microbiology and Its Role in Ruminant Nutrition: Cornell University
JB Russell (2002)
VEGAN, a package of R functions for community ecology
P. Dixon (2003)
Application of New Primer-Enzyme Combinations to Terminal Restriction Fragment Length Polymorphism Profiling of Bacterial Populations in Human Feces
K. Nagashima (2003)
Phylogenetic analysis of fiber-associated rumen bacterial community and PCR detection of uncultured bacteria.
S. Koike (2003)
Evaluation of primers and PCR conditions for the analysis of 16S rRNA genes from a natural environment.
Y. Hongoh (2003)
Application of New PrimerEnzyme Combinations to Terminal Restriction Fragment Length Polymorphism Profiling of Bacterial Populations in
K Nagashima (2003)
Similarity indices, sample size and diversity
H. Wolda (2004)
Effects of peptidic fractions from Saccharomyces cerevisiae culture on growth and metabolism of the ruminal bacteria Megasphaera elsdenii
F. Rossi (2004)
Rumen acidosis: modeling ruminant response to yeast culture.
D. Sauvant (2004)
Alltech's 20th Annual Symposium on Biotechnology in the Feed Industry Re-imagining the feed industry
D Sauvant (2004)
Characterization of Microbial Communities Found in the Human Vagina by Analysis of Terminal Restriction Fragment Length Polymorphisms of 16S rRNA Genes
M. Coolen (2005)
Screening for the effects of natural plant extracts at different pH on in vitro rumen microbial fermentation of a high-concentrate diet for beef cattle.
P. W. Cardozo (2005)
Introducing DOTUR, a Computer Program for Defining Operational Taxonomic Units and Estimating Species Richness
P. Schloss (2005)
Inducing subacute ruminal acidosis in lactating dairy cows.
K. Krause (2005)
Rumen microbial ecosystem
D. N. Kamra (2005)
A new device for measuring kinetics of ruminal pH and redox potential in dairy cattle.
J. P. Marden (2005)
Novel microbial diversity adherent to plant biomass in the herbivore gastrointestinal tract, as revealed by ribosomal intergenic spacer analysis and rrs gene sequencing.
Ross C. Larue (2005)
Effects of yeast culture and galacto-oligosaccharides on ruminal fermentation in holstein cows.
B. Mwenya (2005)
Statistical methods for characterizing diversity of microbial communities by analysis of terminal restriction fragment length polymorphisms of 16S rRNA genes.
Z. Abdo (2006)
Improved serial analysis of V1 ribosomal sequence tags (SARST-V1) provides a rapid, comprehensive, sequence-based characterization of bacterial diversity and community composition.
Z. Yu (2006)
Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences
Weizhong Li (2006)
Plant extracts affect in vitro rumen microbial fermentation.
M. Busquet (2006)
A (2006) Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide
W Li (2006)
Quantification by real‐time PCR of cellulolytic bacteria in the rumen of sheep after supplementation of a forage diet with readily fermentable carbohydrates: effect of a yeast additive
P. Mosoni (2007)
Short pyrosequencing reads suffice for accurate microbial community analysis
Zongzhi Liu (2007)
Pyrosequencing enumerates and contrasts soil microbial diversity
L. F. Roesch (2007)
Evaluation of the bacterial diversity in the feces of cattle using 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP)
S. Dowd (2008)
Comparative Analysis of Human Gut Microbiota by Barcoded Pyrosequencing
A. Andersson (2008)
The Macaque Gut Microbiome in Health, Lentiviral Infection, and Chronic Enterocolitis
P. McKenna (2008)
How does live yeast differ from sodium bicarbonate to stabilize ruminal pH in high-yielding dairy cows?
J. P. Marden (2008)
Accurate taxonomy assignments from 16S rRNA sequences produced by highly parallel pyrosequencers
Zongzhi Liu (2008)
The use of pyrosequencing to identify diet-induced changes in the canine gut microbiome
I. S. Middelbos (2009)
Statistical Methods for Detecting Differentially Abundant Features in Clinical Metagenomic Samples
J. White (2009)
Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities
P. Schloss (2009)
Rumen Bacterial Diversity Dynamics Associated with Changing from Bermudagrass Hay to Grazed Winter Wheat Diets
D. Pitta (2009)
Rumen Microbiome Composition Determined Using Two Nutritional Models of Subacute Ruminal Acidosis
E. Khafipour (2009)
Rumen Microbial Population Dynamics during Adaptation to a High-Grain Diet
S. C. Fernando (2010)

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Rumen microbial responses to supplemental nitrate. II. Potential interactions with live yeast culture on the prokaryotic community and methanogenesis in continuous culture.
C M Welty (2019)
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A. Anadón (2019)
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M.C. Perdomo (2019)
Screening of live yeast and yeast derivatives for their impact of strain and dose on in vitro ruminal fermentation and microbial profiles with varying media pH levels in high-forage beef cattle diet.
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