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Investigation Of Biophysical Mechanisms In Gold Nanoparticle Mediated Laser Manipulation Of Cells Using A Multimodal Holographic And Fluorescence Imaging Setup

S. Kalies, Georgios C. Antonopoulos, M. S. Rakoski, D. Heinemann, M. Schomaker, T. Ripken, H. Meyer
Published 2015 · Materials Science, Medicine

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Laser based cell manipulation has proven to be a versatile tool in biomedical applications. In this context, combining weakly focused laser pulses and nanostructures, e.g. gold nanoparticles, promises to be useful for high throughput cell manipulation, such as transfection and photothermal therapy. Interactions between laser pulses and gold nanoparticles are well understood. However, it is still necessary to study cell behavior in gold nanoparticle mediated laser manipulation. While parameters like cell viability or perforation efficiency are commonly addressed, the influence of the manipulation process on other essential cell parameters is not sufficiently investigated yet. Thus, we set out to study four relevant cell properties: cell volume and area, ion exchange and cytoskeleton structure after gold nanoparticle based laser manipulation. For this, we designed a multimodal imaging and manipulation setup. 200 nm gold nanoparticles were attached unspecifically to canine cells and irradiated by weakly focused 850 ps laser pulses. Volume and area change in the first minute post laser manipulation was monitored using digital holography. Calcium imaging and cells expressing a marker for filamentous actin (F-actin) served to analyze the ion exchange and the cytoskeleton, respectively. High radiant exposures led to cells exhibiting a tendency to shrink in volume and area, possibly due to outflow of cytoplasm. An intracellular raise in calcium was observed and accompanied by an intercellular calcium wave. This multimodal approach enabled for the first time a comprehensive analysis of the cell behavior in gold nanoparticle mediated cell manipulation. Additionally, this work can pave the way for a better understanding and the evaluation of new applications in the context of cell transfection or photothermal therapy.
This paper references
10.1016/J.IJHEATMASSTRANSFER.2008.08.010
Pulsed photothermal heating of the media during bubble generation around gold nanoparticles
D. Lapotko (2009)
10.1038/srep02146
High levels of reactive oxygen species in gold nanoparticle-targeted cancer cells following femtosecond pulse irradiation
Limor Minai (2013)
10.1364/JOSAB.29.001383
Noble-metal nanoparticles and short pulses for nanomanipulations: theoretical analysis
G. Bisker (2012)
10.1152/physrev.00029.2011
Intercellular Ca(2+) waves: mechanisms and function.
L. Leybaert (2012)
10.1088/0957-4484/25/24/245101
Delivery of proteins to mammalian cells via gold nanoparticle mediated laser transfection.
D. Heinemann (2014)
10.1529/BIOPHYSJ.107.113605
Life and times of a cellular bleb.
G. Charras (2008)
10.1021/JA057254A
Cancer cell imaging and photothermal therapy in the near-infrared region by using gold nanorods.
X. Huang (2006)
10.1098/rsif.2014.0071
Single-site sonoporation disrupts actin cytoskeleton organization
X. Chen (2014)
10.1159/000362955
Regulatory Volume Increase and Regulatory Volume Decrease Responses in HL-1 Atrial Myocytes
V. Cacace (2014)
10.1364/AO.41.007437
Fast two-dimensional phase-unwrapping algorithm based on sorting by reliability following a noncontinuous path.
M. A. Herráez (2002)
10.1523/JNEUROSCI.19-02-00520.1999
ATP Released from Astrocytes Mediates Glial Calcium Waves
P. Guthrie (1999)
10.1002/jbio.201000052
Application of dynamic diffractive optics for enhanced femtosecond laser based cell transfection.
M. Antkowiak (2010)
10.1073/PNAS.86.8.2708
Hepatocyte gap junctions are permeable to the second messenger, inositol 1,4,5-trisphosphate, and to calcium ions.
J. Sáez (1989)
10.1021/nn5017742
Comparison of gold nanoparticle mediated photoporation: vapor nanobubbles outperform direct heating for delivering macromolecules in live cells.
R. Xiong (2014)
10.1007/s10237-011-0325-z
Experimental investigation of collagen waviness and orientation in the arterial adventitia using confocal laser scanning microscopy
R. Rezakhaniha (2012)
10.1016/j.jss.2010.10.039
Selective and self-guided micro-ablation of tissue with plasmonic nanobubbles.
E. Lukianova-Hleb (2011)
10.1007/978-3-642-15813-1_9
Coherent Light Imaging and Scattering for Biological Investigations
Huafeng Ding (2011)
10.1117/12.260653
Optical tomography at the microscopic scale by means of a numerical low-coherence holographic technique
E. Cuche (1996)
10.1083/jcb.200602085
Reassembly of contractile actin cortex in cell blebs
G. Charras (2006)
10.1038/nmeth.2089
NIH Image to ImageJ: 25 years of image analysis
C. Schneider (2012)
10.1073/pnas.0903474106
The interaction of TIGIT with PVR and PVRL2 inhibits human NK cell cytotoxicity
N. Stanietsky (2009)
10.1159/000073415
Molecular characterization of the canine HMGB1
H. Murua Escobar (2003)
Digital Holographic Microscopy: Principles, Techniques, and Applications
M. Kim (2011)
10.1111/j.1432-2277.2000.tb02103.x
Mitochondrial defects by intracellular calcium overload versus endothelial cold ischemia/reperfusion injury
E. Gnaiger (2000)
What is nanomedicine? Nanomedicine: Nanotechnology, Biology and Medicine
Freitas RA (2004)
10.1371/journal.pone.0058604
Gold Nanoparticle Mediated Laser Transfection for Efficient siRNA Mediated Gene Knock Down
D. Heinemann (2013)
10.1126/SCIENCE.1411526
Intercellular propagation of calcium waves mediated by inositol trisphosphate.
S. Boitano (1992)
10.1007/978-1-4615-0717-8
Lacrimal Gland, Tear Film, and Dry Eye Syndromes 3
D. Sullivan (2002)
10.1371/journal.pone.0079235
Femtosecond Optoinjection of Intact Tobacco BY-2 Cells Using a Reconfigurable Photoporation Platform
C. Mitchell (2013)
10.1002/cyto.a.20818
A quantitative measure for alterations in the actin cytoskeleton investigated with automated high‐throughput microscopy
J. Weichsel (2010)
10.1007/s10103-007-0470-x
Plasmonic photothermal therapy (PPTT) using gold nanoparticles
X. Huang (2007)
10.1364/OPEX.13.009361
Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy.
B. Rappaz (2005)
10.1016/S0960-9822(03)00358-0
Calcium-induced calcium release
H. Roderick (2003)
10.1161/STROKEAHA.108.528091
Effect of Aging on Elastin Functionality in Human Cerebral Arteries
E. Fonck (2009)
10.2976/1.3073779
Highly thermosensitive Ca2+ dynamics in a HeLa cell through IP3 receptors
V. Tseeb (2009)
10.1529/BIOPHYSJ.105.060590
Cortical actomyosin breakage triggers shape oscillations in cells and cell fragments.
E. Paluch (2005)
10.1038/ncomms1738
Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%
J. Goedhart (2012)
10.1371/journal.pone.0030912
Early Cell Death Detection with Digital Holographic Microscopy
N. Pavillon (2012)
10.1021/CR030698+
Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology.
M. Daniel (2004)
Intercellular Ca2+ waves: mechanisms and function. Physiological reviews
L Leybaert (2012)
10.1021/nl302200w
Plasma mediated off-resonance plasmonic enhanced ultrafast laser-induced nanocavitation.
É. Boulais (2012)
10.1007/978-1-4615-5359-5_98
A novel approach to resolve cellular volume responses to an anisotonic challenge.
P. Iserovich (1998)
10.1063/1.1397255
Generation of calcium waves in living cells by pulsed-laser-induced photodisruption
N. Smith (2001)
10.1117/1.3540674
Simplified approach for quantitative digital holographic phase contrast imaging of living cells.
B. Kemper (2011)
10.1038/nmeth.1220
Lifeact: a versatile marker to visualize F-actin
J. Riedl (2008)
10.1166/JNN.2014.8900
Biological applications of gold nanoparticles.
Monic Shah (2014)
10.1002/jbio.201300056
Plasmonic laser treatment for Morpholino oligomer delivery in antisense applications.
S. Kalies (2014)
10.1016/J.NANO.2004.11.003
What is nanomedicine?
R. Freitas (2005)
10.1007/978-3-642-15813-1
Coherent light microscopy
P. Ferraro (2011)
10.1364/BOE.5.002686
Surface modification of silica particles with gold nanoparticles as an augmentation of gold nanoparticle mediated laser perforation
S. Kalies (2014)
10.5772/15122
Quantitative Analysis of Biological Cells Using Digital Holographic Microscopy
N. Shaked (2011)
10.1016/J.JPHOTOCHEMREV.2012.01.001
Studies on the interaction of pulsed lasers with plasmonic gold nanoparticles toward light manipulation, heat management, and nanofabrication
S. Hashimoto (2012)



This paper is referenced by
10.1038/s41598-018-24908-9
Gold nanoparticle-mediated laser stimulation induces a complex stress response in neuronal cells
S. Johannsmeier (2018)
10.1117/1.JBO.21.5.055001
Biodegradable microsphere-mediated cell perforation in microfluidic channel using femtosecond laser
Atsuhiro Ishii (2016)
Mechanisms for the light-cell interface in optical neurostimulation
S. Johannsmeier (2016)
10.1117/12.2079322
Experimental setup combining digital holographic microscopy (DHM) and fluorescence imaging to study gold nanoparticle mediated laser manipulation
Georgios C. Antonopoulos (2015)
10.7298/X4QJ7FF5
SINGLE CELL MANIPULATION BY FEMTOSECOND LASERS AND VOLUMETRIC ANALYSIS OF CONVECTION ENHANCED DRUG DELIVERY
Poornima Gadamsetty (2017)
10.1364/BOE.8.000177
Modulation of cardiomyocyte activity using pulsed laser irradiated gold nanoparticles.
Lara Gentemann (2017)
Integration of Gap Junction Coupling in Adenosine Signalling of Endothelial Cells
Von der Naturwissenschaftlichen (2018)
10.3390/ijms17081295
Towards Effective Photothermal/Photodynamic Treatment Using Plasmonic Gold Nanoparticles
A. Bucharskaya (2016)
10.1371/journal.pone.0143186
Tile-Based Two-Dimensional Phase Unwrapping for Digital Holography Using a Modular Framework
Georgios C. Antonopoulos (2015)
10.1021/acs.biomac.6b00386
Selective Targeting and Restrictive Damage for Nonspecific Cells by Pulsed Laser-Activated Hyaluronan-Gold Nanoparticles.
Lih-Rou Rau (2016)
10.1080/10717544.2017.1375577
Biological responses to nanomaterials: understanding nano-bio effects on cell behaviors
Xi-Qiu Liu (2017)
10.1117/1.JBO.20.11.115005
Characterization of the cellular response triggered by gold nanoparticle–mediated laser manipulation
S. Kalies (2015)
10.1364/BOE.8.004756
Analysis of poration-induced changes in cells from laser-activated plasmonic substrates.
Nabiha Saklayen (2017)
Laser-Activated Plasmonic Substrates for Intracellular Delivery
Nabiha Saklayen (2017)
10.1039/c9an01869g
Single point single-cell nanoparticle mediated pulsed laser optoporation.
S. Patskovsky (2019)
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