Online citations, reference lists, and bibliographies.
← Back to Search

Transcriptomic Analysis Reveals The Differentially Expressed Genes And Pathways Involved In Drought Tolerance In Pearl Millet [Pennisetum Glaucum (L.) R. Br]

Ambika Dudhate, Harshraj Shinde, Daisuke Tsugama, S. Liu, T. Takano
Published 2018 · Medicine, Biology

Cite This
Download PDF
Analyze on Scholarcy
Pearl millet is a cereal crop known for its high tolerance to drought, heat and salinity stresses as well as for its nutritional quality. The molecular mechanism of drought tolerance in pearl millet is unknown. Here we attempted to unravel the molecular basis of drought tolerance in two pearl millet inbred lines, ICMB 843 and ICMB 863 using RNA sequencing. Under greenhouse condition, ICMB 843 was found to be more tolerant to drought than ICMB 863. We sequenced the root transcriptome from both lines under control and drought conditions using an Illumina Hi-Seq platform, generating 139.1 million reads. Mapping of sequenced reads against the foxtail millet genome, which has been relatively well-annotated, led to the identification of several differentially expressed genes under drought stress. Total of 6799 and 1253 differentially expressed genes were found in ICMB 843 and ICMB 863, respectively. Pathway and gene function analysis by KEGG online tool revealed that the drought response in pearl millet is mainly regulated by pathways related to photosynthesis, plant hormone signal transduction and mitogen-activated protein kinase signaling. The changes in expression of drought-responsive genes determined by RNA sequencing were confirmed by reverse-transcription PCR for 7 genes. These results are a first step to understanding the molecular mechanisms of drought tolerance in pearl millet and lay a foundation for its genetic improvement.
This paper references
Abscisic Acid and Abiotic Stress Signaling
N. Tuteja (2007)
Common Genetic Variation In Cellular Transport Genes and Epithelial Ovarian Cancer (EOC) Risk
G. Chornokur (2015)
Climate Change and Water [Internet]. Climate change and water
B C Bates (2008)
Role of subunits in eukaryotic Photosystem I.
H. Scheller (2001)
Mapping Quantitative Trait Loci Controlling High Iron and Zinc Content in Self and Open Pollinated Grains of Pearl Millet [Pennisetum glaucum (L.) R. Br.]
S. Kumar (2016)
Comparative Leaf and Root Transcriptomic Analysis of two Rice Japonica Cultivars Reveals Major Differences in the Root Early Response to Osmotic Stress
E. Baldoni (2016)
Homologs of Plant PsbP and PsbQ Proteins Are Necessary for Regulation of Photosystem II Activity in the Cyanobacterium Synechocystis 6803
L. Thornton (2004)
Transcriptome Analysis of Flowering Time Genes under Drought Stress in Maize Leaves
Kitae Song (2017)
Glutathione Peroxidase of Pennisetum glaucum (PgGPx) Is a Functional Cd2+ Dependent Peroxiredoxin that Enhances Tolerance against Salinity and Drought Stress
Tahmina Islam (2015)
PsbR, a Missing Link in the Assembly of the Oxygen-evolving Complex of Plant Photosystem II*
M. Suorsa (2006)
Ectopic expression of two AREB / ABF orthologs increase dehydration tolerance in cotton ( Gossypium hirsutum )
Kerr TCC
EXPRESSED SEQUENCE TAGS (ESTs). Bioinforma A Pract Guid to
V Jongeneel (2001)
Plant hormone interactions: innovative targets for crop breeding and management.
S. Wilkinson (2012)
Transcriptional profiling in pearl millet (Pennisetum glaucum L.R. Br.) for identification of differentially expressed drought responsive genes
M. Choudhary (2015)
Engineering model proteins for Photosystem II function
T. Wydrzynski (2007)
KAAS: an automatic genome annotation and pathway reconstruction server
Y. Moriya (1996)
Selection of suitable reference genes for assessing gene expression in pearl millet under different abiotic stresses and their combinations
Radha Shivhare (2016)
Photosynthetic electron flow regulates transcription of the psaB gene in pea (Pisum sativum L.) chloroplasts through the redox state of the plastoquinone pool.
A. Tullberg (2000)
Emission Rate in Plants
B J Atwell
Identification of Drought Tolerant Mechanisms in Maize Seedlings Based on Transcriptome Analysis of Recombination Inbred Lines
Haowei Min (2016)
Climate change and water.
B. C. Bates (2008)
Effect of polyethylene glycol induced drought stress on photosynthesis in two chickpea genotypes with different drought tolerance.
Aykut Saglam (2014)
Physiological and Comparative Proteomic Analysis Reveals Different Drought Responses in Roots and Leaves of Drought-Tolerant Wild Wheat (Triticum boeoticum)
Hui Liu (2015)
Photosynthesis and drought: can we make metabolic connections from available data?
C. Pinheiro (2011)
Pearl millet genome sequence provides a resource to improve agronomic traits in arid environments
R. Varshney (2017)
Molecular character of a phosphatase 2C (PP2C) gene relation to stress tolerance in Arabidopsis thaliana
J. Zhang (2012)
A rapid, non-invasive procedure for quantitative assessment of drought survival using chlorophyll fluorescence
Nick S Woo (2008)
A GH3 family member, OsGH3-2, modulates auxin and abscisic acid levels and differentially affects drought and cold tolerance in rice
H. Du (2012)
Modulating AtDREB1C Expression Improves Drought Tolerance in Salvia miltiorrhiza
T. Wei (2017)
Overexpression of PgDREB2A transcription factor enhances abiotic stress tolerance and activates downstream stress-responsive genes
Parinita Agarwal (2009)
Double overexpression of DREB and PIF transcription factors improves drought stress tolerance and cell elongation in transgenic plants
Madoka Kudo (2017)
Stress signaling in plants: a mitogen-activated protein kinase pathway is activated by cold and drought.
C. Jonak (1996)
Biosynthesis of stress ethylene induced by water deficit.
A. Apelbaum (1981)
RNA-Seq Analysis Reveals MAPKKK Family Members Related to Drought Tolerance in Maize
Y. Liu (2015)
SnRK2 protein kinases--key regulators of plant response to abiotic stresses.
Anna Kulik (2011)
Drought stress and reactive oxygen species
M. C. D. Carvalho (2008)
Reference genome sequence of the model plant Setaria
J. Bennetzen (2012)
Rapid estimates of relative water content.
R. E. Smart (1974)
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
K. Livak (2001)
Exploring Jasmonates in the Hormonal Network of Drought and Salinity Responses
M. Riemann (2015)
Pearl millet [Pennisetum glaucum (L.) R. Br.] consensus linkage map constructed using four RIL mapping populations and newly developed EST-SSRs
V. Rajaram (2012)
Analysis of relative gene expression data using rea l—time quantitative PCR a nd the 2 一 ct method
J. Kenneth (2001)
Abscisic Acid and Abiotic Stress Signaling. Plant Signal Behav [Internet
N Tuteja (2007)
Expressed Sequence Tags (ESTs)
J. Parkinson (2009)
Climate Change and Water: Technical Paper VI
B. C. Bates (2008)
Autonomic markers of emotional processing: skin sympathetic nerve activity in humans during exposure to emotionally charged images
R. Brown (2012)
Ethylene positively regulates cold tolerance in grapevine by modulating the expression of ETHYLENE RESPONSE FACTOR 057
X. Sun (2016)
Ectopic expression of two AREB/ABF orthologs increase dehydration tolerance in cotton (Gossypium hirsutum). Plant Cell Environ
Tcc Kerr (2017)
Effects of water stress on photosystem II photochemistry and its thermostability in wheat plants
C. Lu (1999)
Exploration of Genetic and Genomic Resources for Abiotic and Biotic Stress Tolerance in Pearl Millet
Radha Shivhare (2017)
II.1.5 Phenotyping pearl millet for adaptation to drought
V. Vadez (2012)
Isolation and characterization of expressed sequence tags (ESTs) from subtracted cDNA libraries of Pennisetum glaucum seedlings
R. N. Mishra (2007)
OsIAA6, a member of the rice Aux/IAA gene family, is involved in drought tolerance and tiller outgrowth.
Harin Jung (2015)
Deletion of an Endoplasmic Reticulum Stress Response Element in a ZmPP2C-A Gene Facilitates Drought Tolerance of Maize Seedlings.
Yanli Xiang (2017)
Different response of photosystem II to short and long-term drought stress in Arabidopsis thaliana.
Y. Chen (2016)
Drought and Salt Tolerance in Plants
D. Bartels (2005)
Expressed sequence tags (ESTs).
T. Wolfsberg (2001)
Ectopic expression of two AREB/ABF orthologs increases drought tolerance in cotton (Gossypium hirsutum).
Tyson C. C. Kerr (2018)

This paper is referenced by
Adapting Cereal Grain Crops to Drought Stress: 2020 and Beyond
Tinashe Zenda (2020)
Transcriptomic analysis reveals the mechanism of Picea crassifolia survival for alpine treeline condition
Zheng Shi (2020)
Analysis of transcriptional responses in root tissue of bread wheat landrace (Triticum aestivum L.) reveals drought avoidance mechanisms under water scarcity
M. Chaichi (2019)
Pearl millet stress-responsive NAC transcription factor PgNAC21 enhances salinity stress tolerance in Arabidopsis.
Harshraj Shinde (2019)
Comparative Proteomic and Physiological Analyses of Two Divergent Maize Inbred Lines Provide More Insights into Drought-Stress Tolerance Mechanisms
Tinashe Zenda (2018)
Key Maize Drought-Responsive Genes and Pathways Revealed by Comparative Transcriptome and Physiological Analyses of Contrasting Inbred Lines
Tinashe Zenda (2019)
Comparative transcriptome analysis reveals the genes and pathways involved in terminal drought tolerance in pearl millet
Radha Shivhare (2020)
Millet irrigation with treated wastewater: Gas exchange response and nutrient accumulation
M. Reis (2019)
Comparative de novo transcriptomic profiling of the salinity stress responsiveness in contrasting pearl millet lines
Harshraj Shinde (2018)
SMRT sequencing of a full-length transcriptome reveals transcript variants involved in C18 unsaturated fatty acid biosynthesis and metabolism pathways at chilling temperature in Pennisetum giganteum
Q. Li (2020)
Comparative transcriptomic and physiological analyses of contrasting hybrid cultivars ND476 and ZX978 identify important differentially expressed genes and pathways regulating drought stress tolerance in maize
G. Liu (2020)
Comparative analysis of two sister Erythrophleum species (Leguminosae) reveal contrasting transcriptome-wide responses to early drought stress.
Mohamed Neji (2019)
De novo assembly and comparative transcriptome analysis of contrasting pearl millet (Pennisetum glaucum L.) genotypes under terminal drought stress using illumina sequencing
Radha Shivhare (2020)
Differential gene expression and gene ontologies associated with increasing water-stress in leaf and root transcriptomes of perennial ryegrass (Lolium perenne)
Albert Fradera-Sola (2019)
Omics for proso millet genetic improvement
Rituraj Khound (2020)
Small RNA sequencing reveals the role of pearl millet miRNAs and their targets in salinity stress responses
Harshraj Shinde (2020)
Semantic Scholar Logo Some data provided by SemanticScholar