Online citations, reference lists, and bibliographies.
← Back to Search

Proteomic Analysis By Multidimensional Protein Identification Technology.

L. Florens, M. Washburn
Published 2006 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Multidimensional chromatography coupled to mass spectrometry is an emerging technique for the analysis of complex protein mixtures. One approach in this general category, multidimensional protein identification technology (MudPIT), couples biphasic or triphasic microcapillary columns to high-performance liquid chromatography, tandem mass spectrometry, and database searching. The integration of each of these components is critical to the implementation of MudPIT in a laboratory. MudPIT can be used for the analysis of complex peptide mixtures generated from biofluids, tissues, cells, organelles, or protein complexes. The information described in this chapter will provide researchers with details for sample preparation, column assembly, and chromatography parameters for complex peptide mixture analysis.
This paper references

This paper is referenced by
Genomic and Proteomic Analysis of phiEco 32 , a Novel Escherichia coli Phage
Dhruti Savalia (2008)
Use of sequential chemical extractions to purify nuclear membrane proteins for proteomics identification.
Nadia Korfali (2009)
The Spliceosomal Protein SF3B5 is a Novel Component of Drosophila SAGA that Functions in Gene Expression Independent of Splicing.
R. Stegeman (2016)
Putative Rust Fungal Effector Proteins in Infected Bean and Soybean Leaves.
B. Cooper (2016)
Relative, label-free protein quantitation: Spectral counting error statistics from nine replicate MudPIT samples
B. Cooper (2010)
Peer Reviewed Title: Polysome profiling reveals translational control of gene expression in the human malaria parasite Plasmodium falciparum
Le Roch (2013)
Lentiviral Vpx Accessory Factor Targets VprBP/DCAF1 Substrate Adaptor for Cullin 4 E3 Ubiquitin Ligase to Enable Macrophage Infection
S. Srivastava (2008)
Aneuploidy confers quantitative proteome changes and phenotypic variation in budding yeast
Norman Pavelka (2010)
Improved enrichment and proteomic analysis of brain proteins with signaling function by heparin chromatography.
K. Krapfenbauer (2009)
Prevention of Treacher Collins syndrome craniofacial anomalies in mouse models via maternal antioxidant supplementation
D. Sakai (2016)
Merkel Cell Polyomavirus Small T Antigen Perturbs the Cellular DNA Damage Response
Rosa Yoon (2015)
Structural changes in TAF4b-TFIID correlate with promoter selectivity.
Wei-li Liu (2008)
FBXL2- and PTPL1-mediated degradation of p110-free p85β regulatory subunit controls the PI(3)K signalling cascade
S. Kuchay (2013)
Proteomics of weakly bound cell wall proteins in rice calli.
Xiong-Yan Chen (2009)
DYRK1A protein kinase promotes quiescence and senescence through DREAM complex assembly.
Larisa Litovchick (2011)
The chromatin bound proteome of the human malaria parasite
G. Batugedara (2020)
STRIPAK directs PP2A activity toward MAP4K4 to promote oncogenic transformation
J. Kim (2019)
Proteomics in Toxicology
Tanzila Rehman (2014)
A systems chemoproteomic analysis of acyl-CoA/protein interaction networks
M. Levy (2019)
Determining Protein Complex Connectivity Using a Probabilistic Deletion Network Derived from Quantitative Proteomics
Mihaela E. Sardiu (2009)
Analyzing chromatin remodeling complexes using shotgun proteomics and normalized spectral abundance factors.
L. Florens (2006)
Controlling for Gene Expression Changes in Transcription Factor Protein Networks*
C. S. Banks (2014)
A novel histone fold domain-containing protein that replaces TAF6 in Drosophila SAGA is required for SAGA-dependent gene expression.
V. Weake (2009)
The Genetic and Physical Interactomes of the Saccharomyces cerevisiae Hrq1 Helicase
Cody M Rogers (2020)
Regulates Retention of G 9 a on Chromatin 1 A Role for Widely Interspaced Zinc Finger ( WIZ ) in Retention of the G 9 a Methyltransferase on Chromatin *
Jeremy M. Simon (2015)
Temporal regulation of gene expression of the Thermus thermophilus bacteriophage P23-45.
Z. Berdygulova (2011)
Proteomic and Genomic Analyses of the Rvb1 and Rvb2 Interaction Network upon Deletion of R2TP Complex Components*
M. Lakshminarasimhan (2016)
Pathway Proteomics and Chemical Proteomics Team Up in Drug Discovery
C. Hopf (2007)
Analysis of the Arabidopsis floral proteome: detection of over 2 000 proteins and evidence for posttranslational modifications.
Baomin Feng (2009)
FBH1 promotes DNA double-strand breakage and apoptosis in response to DNA replication stress
Yeon-Tae Jeong (2013)
The Human Cytoplasmic Dynein Interactome Reveals Novel Activators of Motility
W. B. Redwine (2017)
gondiiCortical Microtubules of Toxoplasma Components of a Protein Complex Coating Novel Thioredoxin-Like Proteins Are
S. Ems-McClung (2014)
See more
Semantic Scholar Logo Some data provided by SemanticScholar