Online citations, reference lists, and bibliographies.
← Back to Search

Solid Lipid Nanoparticle (SLN) Formulations As A Potential Tool For The Reduction Of Cytotoxicity Of Saponins.

H. Van de Ven, M. Vermeersch, T. Shunmugaperumal, J. Vandervoort, L. Maes, A. Ludwig
Published 2009 · Chemistry, Medicine

Cite This
Download PDF
Analyze on Scholarcy
The present pilot study explored the potential of solid lipid nanoparticles (SLN) to entrap saponins and reduce the membrane toxicity of these compounds. SLN composed of different types of solid lipid were prepared by the cold homogenisation technique. Combinations of anionic, cationic and non-ionic stabilisers were selected in order to obtain negatively, positively and neutrally charged SLN. Mean particle size and zeta potential of blank and saponin-loaded formulations were measured by Dynamic Light Scattering (DLS), Electrophoretic Light Scattering (ELS) and in vitro cytotoxicity on MRC-5 SV2 and J774 cells was assessed using a resazurin-based assay. The type of solid lipid used for the formulation influenced the mean particle size, while the zeta potential mainly depended on the kind of surfactant utilised. Blank SLN composed of hard fat and anionic or non-ionic surfactants did not result in cytotoxicity. After loading with saponin, the anionic hard fat SLN was found to be the optimal formulation.
This paper references
Effect of lipid matrix and size of solid lipid nanoparticles (SLN) on the viability and cytokine production of macrophages.
N. Schöler (2002)
Effect of surface properties of liposomes coated with a modified polyvinyl alcohol (PVA-R) on the interaction with macrophage cells.
K. Nakano (2008)
Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.
T. Mosmann (1983)
An improved resazurin-based cytotoxicity assay for hepatic cells
M. McMillian (2004)
Intracellular trafficking pathways and drug delivery: fluorescence imaging of living and fixed cells.
P. Watson (2005)
Preparation, characterization and physico-chemical properties of solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC): their benefits as colloidal drug carrier systems.
M. Uner (2006)
Solid lipid nanoparticles (SLN) for controlled drug delivery - a review of the state of the art.
R. Mueller (2000)
Influence of polysaccharide coating on the interactions of nanoparticles with biological systems.
C. Lemarchand (2006)
New pentacyclic triterpene saponins with strong anti-leishmanial activity from the leaves of Maesa balansae
N. Germonprez (2004)
Phagocytic uptake of fluorescent stealth and non-stealth solid lipid nanoparticles
C. Bocca (1998)
Flow cytometric evaluation of a model for phagocytosis of cells undergoing apoptosis.
D. Schrijvers (2004)
Solid lipid nanoparticles: production, characterization and applications.
W. Mehnert (2001)
Surfactant, but not the size of solid lipid nanoparticles (SLN) influences viability and cytokine production of macrophages.
N. Schöler (2001)
Cellular uptake of solid lipid nanoparticles and cytotoxicity of encapsulated paclitaxel in A549 cancer cells.
H. Yuan (2008)
Lipid nanoparticles for alkyl lysophospholipid edelfosine encapsulation: development and in vitro characterization.
A. Estella-Hermoso de Mendoza (2008)
In Vitro and In Vivo Activities of a Triterpenoid Saponin Extract (PX-6518) from the Plant Maesa balansae against Visceral Leishmania Species
L. Maes (2004)
Cytotoxicity studies of Dynasan 114 solid lipid nanoparticles (SLN) on RAW 264.7 macrophages—impact of phagocytosis on viability and cytokine production
C. Olbrich (2004)
Comparison of the efficacies of various formulations of amphotericin B against murine visceral leishmaniasis.
A. Mullen (1997)
Protective effect of SLNs encapsulation on the photodegradation and thermal degradation of retinyl palmitate introduced in hydroxyethylcellulose gel
S. Sapino (2005)
Liposome-based drug delivery to alveolar macrophages
S. Vyas (2007)
Induction of macrophage apoptosis by ceramic and polyethylene particles in vitro.
I. Catelas (1999)
Targeted pharmaceutical nanocarriers for cancer therapy and imaging
V. Torchilin (2008)
High-throughput cytotoxicity screening: hit and miss
S. Crouch (2001)
Parasitic diseases: Liposomes and polymeric nanoparticles versus lipid nanoparticles.
A. Date (2007)
Solid lipid nanoparticles for parenteral drug delivery.
S. Wissing (2004)
A comparison of the activities of three amphotericin B lipid formulations against experimental visceral and cutaneous leishmaniasis.
V. Yardley (2000)
Molecular Structures of Saponins from Quillaja saponaria Molina
D. V. Setten (1996)

This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar