Online citations, reference lists, and bibliographies.
← Back to Search

Upregulation Of Macrophage Lipoprotein Lipase In Patients With Type 2 Diabetes: Role Of Peripheral Factors.

M. Sartippour, G. Renier
Published 2000 · Medicine, Biology

Cite This
Download PDF
Analyze on Scholarcy
Atherosclerosis is the major complication of diabetes. Accumulating evidence indicates that lipoprotein lipase (LPL) produced by macrophages in the vascular wall may favor the development of atherosclerosis by promoting lipid accumulation within the lesion. We previously demonstrated that high glucose stimulates in vitro murine and human macrophage LPL production. In this study, we measured macrophage LPL mRNA expression, immunoreactive mass, and activity in normotriglyceridemic subjects with type 2 diabetes. Monocytes isolated from healthy control subjects and patients with type 2 diabetes were differentiated into macrophages in RPMI medium containing 20% autologous serum. After 5 days in culture, macrophage LPL mRNA expression, mass, and activity were determined. Macrophages of diabetic patients cultured in their own sera showed a significant increase in LPL mRNA levels, mass, and activity compared with macrophages of control subjects. Differentiation of macrophages of diabetic patients in sera obtained from control subjects significantly reduced these anomalies. Conversely, culturing macrophages of control subjects in sera of diabetic patients significantly increased LPL mass and activity in these cells. Besides LPL overproduction, macrophages of diabetic patients exhibited an increase in basal and LPL-induced tumor necrosis factor (TNF)-alpha release. TNF-alpha alterations were reduced by exposing these cells to sera of control subjects. Overall, these data demonstrate that macrophages of diabetic patients overexpress LPL and TNF-alpha and that peripheral factors dysregulated in diabetes are, at least in part, responsible for these alterations.
This paper references
The role of the monocyte in atherogenesis: I. Transition of blood-borne monocytes into foam cells in fatty lesions.
R. Gerrity (1981)
Lipoprotein lipase enhances human monocyte adhesion to aortic endothelial cells.
J. Mamputu (1997)
Lipoprotein lipase in human monocyte-derived macrophages isolated from type II diabetic subjects.
G. Creedon (1997)
Effect of improved diabetes control on the expression of lipoprotein lipase in human adipose tissue.
R. Simsolo (1992)
Lipoprotein lipase can function as a monocyte adhesion protein.
J. Obunike (1997)
Role of oxidant injury on macrophage lipoprotein lipase (LPL) production and sensitivity to LPL.
G. Renier (1996)
Macrophage-derived foam cells freshly isolated from rabbit atherosclerotic lesions degrade modified lipoproteins, promote oxidation of low-density lipoproteins, and contain oxidation-specific lipid-protein adducts.
M. Rosenfeld (1991)
Augmented Production of Tumor Necrosis Factor-α in Obese Mice
T. Yamakawa (1995)
Advanced glycosylation end products in tissue and the biochemical basis of diabetic complications.
M. Brownlee (1988)
Expression of lipoprotein lipase mRNA and secretion in macrophages isolated from human atherosclerotic aorta.
L. Mattsson (1993)
Human Monocytes/Macrophages Release TNF-α in Response to Ox-LDL
S. Jovinge (1996)
Lipoprotein lipase stimulates the binding and uptake of moderately oxidized low-density lipoprotein by J774 macrophages.
W. L. Hendriks (1996)
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
P. Chomczyński (1987)
A sandwich-enzyme immunoassay for the quantification of lipoprotein lipase and hepatic triglyceride lipase in human postheparin plasma using monoclonal antibodies to the corresponding enzymes.
Y. Ikeda (1990)
Induction of tumor necrosis factor alpha gene expression by lipoprotein lipase.
G. Renier (1994)
Lipoprotein lipase increases low density lipoprotein retention by subendothelial cell matrix.
U. Saxena (1992)
Relationship of Glucose Tolerance and Plasma Insulin to the Incidence of Coronary Heart Disease: Results from Two Population Studies in Finland
K. Pyörälä (1979)
Stimulatory effect of glucose on macrophage lipoprotein lipase expression and production.
M. Sartippour (1998)
Mechanisms by which lipoprotein lipase alters cellular metabolism of lipoprotein(a), low density lipoprotein, and nascent lipoproteins. Roles for low density lipoprotein receptors and heparan sulfate proteoglycans.
K. Williams (1992)
Lipoprotein lipase activity in adipose tissue and skeletal muscle of human diabetics during insulin deprivation and restoration.
M. R. Taskinen (1981)
Lipid peroxide level in plasma of diabetic patients.
Y. Sato (1979)
High macrophage lipoprotein lipase expression and secretion are associated in inbred murine strains with susceptibility to atherosclerosis.
G. Renier (1993)
Human monocytes in culture synthesize and secrete lipoprotein lipase.
P. Wang-Iverson (1982)
Role of Oxidative Stress in Development of Complications in Diabetes
J. Baynes (1991)
Lipoprotein lipase is synthesized by macrophage-derived foam cells in human coronary atherosclerotic plaques.
K. O'brien (1992)
Enhanced lipoprotein lipase secretion from human monocyte-derived macrophages caused by hypertriglyceridemic very low density lipoproteins.
S. Ishibashi (1989)
Lipoprotein lipase enhances binding of lipoproteins to heparan sulfate on cell surfaces and extracellular matrix.
S. Eisenberg (1992)
Reactive oxygen species facilitate the in vitro and in vivo lipopolysaccharide-induced release of tumor necrosis factor.
G. Chaudhri (1989)
The Effect of Glucose and Advanced Glycosylation End Products on IL‐6 Production by Human Monocytes
M. Morohoshi (1995)
Serum Lipoprotein and Lipoprotein Lipase in Overweight, Type II Diabetics during and after Supplemented Fasting
B. Vessby (1985)
New fluorogenic triacylglycerol analogs as substrates for the determination and chiral discrimination of lipase activities.
M. Duque (1996)
Lipoprotein lipase activity in skeletal muscle is related to insulin sensitivity.
T. Pollare (1991)
In vitro production of interleukin-1, interleukin-6, and tumor necrosis factor-alpha in insulin-dependent diabetes mellitus.
Y. Ohno (1993)
Cachectin/TNF and IL-1 induced by glucose-modified proteins: role in normal tissue remodeling.
H. Vlassara (1988)
Differentiation of Human Monocytes to Monocyte-Derived Macrophages Is Associated With Increased Lipoprotein Lipase–Induced Tumor Necrosis Factor-α Expression and Production
Jean-ClaudeMamputu (1999)
Lipoprotein lipase. A multifunctional enzyme relevant to common metabolic diseases.
R. Eckel (1989)
Lipoprotein lipase-mediated uptake and degradation of low density lipoproteins by fibroblasts and macrophages.
S. Rumsey (1992)
Lipoprotein lipase secretion by human monocyte-derived macrophages.
A. Chait (1982)
Effect of cholesterol feeding on arterial lipolytic activity in the rabbit.
J. Corey (1977)
Change in skeletal muscle lipoprotein lipase activity in response to insulin/glucose in non-insulin-dependent diabetes mellitus.
T. J. Yost (1995)
Insulin Deficiency Decreases Lipoprotein Lipase Secretion by Murine Macrophages
S. Behr (1988)
Spontaneous aggregation as a mechanism for human monocyte purification.
S. Mentzer (1986)
Insulin Resistance: A Multifaceted Syndrome Responsible for NIDDM, Obesity, Hypertension, Dyslipidemia, and Atherosclerotic Cardiovascular Disease
R. DeFronzo (1991)
The role of the monocyte in atherogenesis: I. Transition of blood-borne monocytes into foam cells in fatty lesions.
Gerrity Rg (1981)
J. Fuller (1980)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)

This paper is referenced by
Astragalus membranaceus Inhibits Inflammation via Phospho-P38 Mitogen-Activated Protein Kinase (MAPK) and Nuclear Factor (NF)-κB Pathways in Advanced Glycation End Product-Stimulated Macrophages
Qiaojing Qin (2012)
Suppression of endothelial or lipoprotein lipase in THP-1 macrophages attenuates proinflammatory cytokine secretions⃞ Published, JLR Papers in Press, November 8, 2006.
Guosong Qiu (2007)
Lipoprotein Lipase in the Arterial Wall: Linking LDL to the Arterial Extracellular Matrix and Much More
M. Pentikäinen (2002)
Diabetic periodontitis: a model for activated innate immunity and impaired resolution of inflammation.
Hamdy A. Nassar (2007)
Structure, function and regulation of the ABC1 gene product
G. Schmitz (2001)
Unsaturated Fatty Acids Inhibit Cholesterol Efflux from Macrophages by Increasing Degradation of ATP-binding Cassette Transporter A1*
Y. Wang (2002)
Peroxisome proliferator-activated receptor α and γ agonists upregulate human macrophage lipoprotein lipase expression
L. Li (2002)
Macrophage-derived lipoprotein lipase increases aortic atherosclerosis in cholesterol-fed Tg rabbits.
T. Ichikawa (2005)
An investigation of the atherogenicity of intermediate density lipoprotein in people with and without type 2 diabetes mellitus
Ching-Loong Cheung (2009)
Regulation of smooth muscle cell accumulation in diabetes-accelerated atherosclerosis.
B. Askari (2002)
Lipoprotein lipase: the regulation of tissue specific expression and its role in lipid and energy metabolism
Karina Preiss-Landl (2002)
Leptin increases lipoprotein lipase secretion by macrophages: involvement of oxidative stress and protein kinase C.
F. Maingrette (2003)
Phosphorylation of Pleckstrin Increases Proinflammatory Cytokine Secretion by Mononuclear Phagocytes in Diabetes Mellitus1
Yong Ding (2007)
Advanced glycation end products potentiate the stimulatory effect of glucose on macrophage lipoprotein lipase expression Published, JLR Papers in Press, June 21, 2004. DOI 10.1194/jlr.M400169-JLR200
M. Beauchamp (2004)
Insulin Receptor Substrate-2-dependent Interleukin-4 Signaling in Macrophages Is Impaired in Two Models of Type 2 Diabetes Mellitus*
M. E. Hartman (2004)
Lipoprotein Lipase in the Arterial Wall: Regulation by Dietary Fatty Acids
C. Chang (2011)
Direct regulatory effect of fatty acids on macrophage lipoprotein lipase: potential role of PPARs.
S. E. Michaud (2001)
Homocysteine induces protein kinase C activation and stimulates c-Fos and lipoprotein lipase expression in macrophages.
M. Beauchamp (2002)
Severe hypertriglyceridaemia in a Greek infant: a clinical, biochemical and genetic study
E. Kavazarakis (2004)
Role of diabetes, hypertension, and cigarette smoking on atherosclerosis
R. K. Mathur (2010)
Atorvastatin decreases lipoprotein lipase and endothelial lipase expression in human THP-1 macrophages Published, JLR Papers in Press, July 20, 2007.
Guosong Qiu (2007)
Glycosaminoglycan metabolism defects and atherosclerosis: frequent association of endothelial dysfunction in patients with Mucopolysaccharidosis
S. Yano (2013)
Enhanced lipoprotein lipase secretion and foam cell formation by macrophages of patients with growth hormone deficiency: possible contribution to increased risk of atherogenesis?
O. Serri (2004)
A critical role for the Sp1-binding sites in the transforming growth factor-β-mediated inhibition of lipoprotein lipase gene expression in macrophages
S. A. Irvine (2005)
Linoleic acid increases lectin-like oxidized LDL receptor-1 (LOX-1) expression in human aortic endothelial cells.
F. Maingrette (2005)
Lipoprotein lipase: structure, function, regulation, and role in disease
J. R. Mead (2002)
Soluble tumor necrosis factor receptor 2 is independently associated with pulse wave velocity in nonobese Japanese patients with type 2 diabetes mellitus.
M. Ohgushi (2007)
Régulation de la lipoprotéine lipase macrophagique et de LOX-1 par des facteurs métaboliques. Implications dans l’athérosclérose associée au diabète de type 2.
Fritz Maingrette (2009)
High-Energy Diets, Fatty Acids and Endothelial Cell Function: Implications for Atherosclerosis
B. Hennig (2001)
n-3 Fatty Acids Decrease Arterial Low-Density Lipoprotein Cholesterol Delivery and Lipoprotein Lipase Levels in Insulin-Resistant Mice
C. Chang (2010)
n-3 Fatty Acids Reduce Arterial LDL-Cholesterol Delivery and Arterial Lipoprotein Lipase Levels and Lipase Distribution
C. Chang (2009)
Gestational and hormonal regulation of human placental lipoprotein lipase Published, JLR Papers in Press, August 22, 2006.
A. L. Magnusson-Olsson (2006)
See more
Semantic Scholar Logo Some data provided by SemanticScholar