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Preparation And In Vitro–in Vivo Evaluation Of Salmon Calcitonin-loaded Polymeric Nanoparticles

Eliza Główka, A. Sapin-Minet, P. Leroy, J. Lulek, P. Maincent
Published 2010 · Medicine, Materials Science

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The aim of the study was to develop and characterize polymeric nanoparticles as a sustained release system for salmon calcitonin (sCT). Nanoparticles were prepared by a double emulsion solvent evaporation method using Eudragit®RS and two types of a biodegradable poly(lactic-co-glycolic) copolymer (PLGA). It was demonstrated that sCT was incorporated into nanoparticles with encapsulation efficiencies in the range 69–83%. In vitro release studies, unconventionally conducted in 5% acetic acid, showed great differences in sCT release time profiles. Nanoparticles with fast release profile (Eudragit®RS, PLGA/Eudragit®RS) released 80–100% of the encapsulated drug within a few hours. In contrast, the sCT release from pure PLGA nanoparticles was very slow, incomplete and reached only 20% after 4 weeks. In vivo study, conducted in Wistar rats, proved that elevated serum sCT levels could be sustained for 3 days after subcutaneous administration of PLGA nanoparticles and the achieved bioavailability was increased compared to sCT solution.
This paper references
Evaluation of ciprofloxacin-loaded Eudragit RS100 or RL100/PLGA nanoparticles.
K. Dillen (2006)
Release of tetanus toxoid from adjuvants and PLGA microspheres: how experimental set-up and surface adsorption fool the pattern.
P. Johansen (1998)
Forty years of calcitonin--where are we now? A tribute to the work of Iain Macintyre, FRS.
M. Zaidi (2002)
In vitro and in vivo characterization of huperzine a loaded microspheres made from end-group uncapped poly(d,l-lactide acid) and poly(d,l-lactide-co-glycolide acid).
Ping Gao (2006)
Factors affecting the in vitro release of recombinant human interferon‐γ (rhIFN‐γ) from PLGA microspheres
J. Yang (1997)
One-month sustained release microspheres of 125I-bovine calcitonin. In vitro-in vivo studies.
R. Diaz (1999)
Polyisobutylcyanoacrylate nanoparticles as sustained release system for calcitonin
C. Tasset (1994)
Assessment of protein release kinetics, stability and protein polymer interaction of lysozyme encapsulated poly(D,L-lactide-co-glycolide) microspheres.
G. Jiang (2002)
Calcitonin for the long-term prevention and treatment of postmenopausal osteoporosis.
J. Body (2002)
Calcitonin Therapy in Osteoporosis
M. Muñoz-Torres (2004)
Adsorption of peptides to poly(D,L-lactide-co-glycolide): 2. Effect of solution properties on the adsorption
Tsuimin Tsai (1996)
One-month sustained release microspheres
RV Diaz (1999)
Peptide acylation by poly(alpha-hydroxy esters).
A. Lucke (2002)
Effect of the type of hydrophobic polymers on the size of nanoparticles obtained by emulsification–solvent evaporation
Yulia V. Chernysheva (2003)
Treatment of bone in elderly subjects: calcium, vitamin D, fluor, bisphosphonates, calcitonin.
J. Reginster (1995)
Stability aspects of salmon calcitonin entrapped in poly(ether-ester) sustained release systems.
R. van Dijkhuizen-Radersma (2002)
In vivo assessment of salmon calcitonin sustained release from biodegradable microspheres
K. C. Lee (1991)
Degradation of Synthetic Salmon Calcitonin in Aqueous Solution
K. Lee (2004)
Oral delivery of insulin associated to polymeric nanoparticles in diabetic rats.
C. Damgé (2007)
Analgesic effect of calcitonin in osteoporosis.
C. Gennari (2002)
Early stages of salmon calcitonin aggregation: effect induced by ageing and oxidation processes in water and in the presence of model membranes.
M. C. Gaudiano (2005)
Utility of pharmacodynamic measures for assessing the oral bioavailability of peptides. 1. Administration of recombinant salmon calcitonin in rats.
P. Sinko (1995)
Adsorption of calcitonin to glass.
S. Law (1999)
Factors affecting the in vitro release of recombinant human interferon-gamma (rhIFN-gamma) from PLGA microspheres.
J. Yang (1997)
Compression, compaction, and disintegration properties of low crystallinity celluloses produced using different agitation rates during their regeneration from phosphoric acid solutions
V. Kumar (2008)
Peptide containing microspheres from low molecular weight and hydrophilic poly(d,l-lactide-co-glycolide)
R. Mehta (1996)
Peptide Acylation by Poly(α-Hydroxy Esters)
A. Lucke (2004)
Oral delivery of salmon calcitonin.
Y. H. Lee (2000)
Preparation and In Vitro Evaluation of Heparin-Loaded Polymeric Nanoparticles
Y. Jiao (2001)
Effect of acidic pH on PLGA microsphere degradation and release.
B. S. Zolnik (2007)
Preparation, characterization, and in vivo evaluation of salmon calcitonin microspheres
B. Dani (2008)
Importance of the test medium for the release kinetics of a somatostatin analogue from poly(D,L-lactide-co-glycolide) microspheres.
M. Blanco-Prieto (1999)
Low Molecular Weight Heparin-Loaded Polymeric Nanoparticles: Formulation, Characterization, and Release Characteristics
V. Hoffart (2002)
Calcitonin release system in the treatment of experimental osteoporosis. Histomorphometric evaluation.
N. N. Aldini (2001)
Development and characterization of protein-loaded poly(lactide-co-glycolide) nanospheres
M. D. Blanco (1997)
Preparation and in vitro evaluation of heparinloaded polymeric nanoparticles
YY Jiao (2001)

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R. Yang (2011)
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A. Ghassemi (2011)
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S. Maher (2009)
pH-responsive nanoparticles releasing tenofovir intended for the prevention of HIV transmission.
T. Zhang (2011)
Microspheres based on biodegradable functionalized poly(alpha-hydroxy) acids for the controlled release of bioactive proteins and peptides
Amir H. Ghassemi (2011)
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P. Yu (2019)
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Ruixue Wu (2017)
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Ghobad Mohammadi (2017)
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Kelsen Bastari (2013)
Self-assembled hyaluronate/protamine polyelectrolyte nanoplexes: synthesis, stability, biocompatibility and potential use as peptide carriers.
A. Umerska (2014)
鲑鱼降钙素PLGA微球缓释载体 Salmon Calcitonin-Loaded PLGA Microspheres as a Sustained-Release Vehicle
吴瑞雪 (2017)
Recent developments in protein and peptide parenteral delivery approaches.
Ashaben Patel (2014)
Intermolecular interactions between salmon calcitonin, hyaluronate, and chitosan and their impact on the process of formation and properties of peptide-loaded nanoparticles.
A. Umerska (2014)
Design and evaluation of poly(DL-lactic-co-glycolic acid) nanocomposite particles containing salmon calcitonin for inhalation.
M. Yang (2012)
Hollow Microparticles as a Superior Delivery System over Solid Microparticles for the Encapsulation of Peptides
Sharad Kharel (2018)
Design and evaluation of solid lipid nanoparticles modified with peptide ligand for oral delivery of protein drugs.
T. Fan (2014)
Bacterial Nanocellulose as a Microbiological Derived Nanomaterial
A. Stanisławska (2016)
Strategies for the nanoencapsulation of hydrophilic molecules in polymer-based nanoparticles.
S. Vrignaud (2011)
Drug carriers in osteoporosis: preparation, drug encapsulation and applications.
K. Miladi (2013)
Polymeric nanoparticle formulation of octapeptide (NP-OP): in vitro release and in vivo effect in common marmosets, Callithrix jacchus Linn.
T. Nandedkar (2013)
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