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Use Of Animal Protein-free Products For Passaging Adherent Human Adipose-derived Stromal/stem Cells.

Pedro P. Carvalho, Xiying Wu, Gang Yu, Marilyn A. Dietrich, Isabel Rosa Dias, Manuela E Gomes, Rui Luís Reis, Jeffrey M Gimble
Published 2011 · Medicine, Biology
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Adherent adipose-derived stromal/stem cells (ASC) have been used in pre-clinical regenerative medical studies applied to a broad range of tissues with an ultimate goal of translating these findings to clinical safety and efficacy testing; however, many protocols passage the cells using porcine-derived trypsin. We have compared porcine trypsin with animal protein-free products from recombinant bacteria (TrypLE Express; Invitrogene) and corn (TrypZean; Sigma) based on cell yield, viability and immunophenotype. ASC harvested with each trypsin product were comparable.
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