Use Of Animal Protein-free Products For Passaging Adherent Human Adipose-derived Stromal/stem Cells.
Pedro P. Carvalho, Xiying Wu, Gang Yu, Marilyn A. Dietrich, Isabel Rosa Dias, Manuela E Gomes, Rui Luís Reis, Jeffrey M Gimble
Published 2011 · Medicine, Biology
Download PDFAnalyze on Scholarcy
Adherent adipose-derived stromal/stem cells (ASC) have been used in pre-clinical regenerative medical studies applied to a broad range of tissues with an ultimate goal of translating these findings to clinical safety and efficacy testing; however, many protocols passage the cells using porcine-derived trypsin. We have compared porcine trypsin with animal protein-free products from recombinant bacteria (TrypLE Express; Invitrogene) and corn (TrypZean; Sigma) based on cell yield, viability and immunophenotype. ASC harvested with each trypsin product were comparable.
This paper references
A simplified method of trypsinization for tissue cell dispersion
Dobrova In (1959)
The effect of proteolytic enzymes on isolated adipose cells.
Jen Feng Kuo (1966)
A Defined and Xeno-Free Culture Method Enabling the Establishment of Clinical-Grade Human Embryonic, Induced Pluripotent and Adipose Stem Cells
Kristiina Rajala (2010)
Novel maxillary reconstruction with ectopic bone formation by GMP adipose stem cells.
Karri Mesimäki (2009)
[Cell dispersion by trypsin at low temperatures].
P Sheridan Daniel (1957)
Attachment and growth of human embryonic stem cells on microcarriers.
Blaine Wesley Phillips (2008)
In vitro culture and expansion of human limbal epithelial cells
Indumathi Mariappan (2010)
Mechanisms of cell adhesion: early-forming junctions between aggregating fibroblasts.
Clive W. Lloyd (1976)
Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24.
David M. Panchision (2007)
Isolation of human adipose-derived stem cells from biopsies and liposuction specimens.
Severine G. Dubois (2008)
MHC-restricted cytotoxic T-lymphocyte assay: an improved method based on normal and SV40-immortalized rabbit epidermal target cells.
Eleana Pozzi (2009)
[Improved model of an apparatus for trysinization of tissues for cell cultures].
Georges Barski (1956)
Comparison of a recombinant trypsin with the porcine pancreatic extract on sperm used for the in vitro production of bovine embryos.
K. J. Mattson (2008)
Effect of Porcine Proinsulin in Vitro on Adipose Tissue and Diaphragm of the Normal Rat
Walter Norman Shaw (1968)
A microcarrier cell culture process for propagating rabies virus in Vero cells grown in a stirred bioreactor under fully animal component free conditions.
Samia Rourou (2007)
Efficacy of a recombinant trypsin product against bovine herpesvirus 1 associated with in vivo- and in vitro-derived bovine embryos.
Mylissa S D Marley (2008)
Development of an animal-component free medium for vero cells culture.
Samia Rourou (2009)
Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement.
Massimiliano Dominici (2006)
Serum-free, xeno-free culture media maintain the proliferation rate and multipotentiality of adipose stem cells in vitro.
Bettina Lindroos (2009)
Surface protein characterization of human adipose tissue-derived stromal cells.
Stan Gronthos (2001)
Differentiation of adipose stem cells.
Bruce A Bunnell (2008)
Clinical and preclinical translation of cell-based therapies using adipose tissue-derived cells
Jeffrey M Gimble (2010)
Yield and characterization of subcutaneous human adipose-derived stem cells by flow cytometric and adipogenic mRNA analyzes.
Gang Yu (2010)
Current technology for the derivation of pluripotent stem cell lines from human embryos.
Kouichi Hasegawa (2010)
Human adipose-derived stromal cells accelerate diabetic wound healing: impact of cell formulation and delivery.
Peter J. Amos (2010)
This paper is referenced by
Undifferentiated human adipose-derived stromal/stem cells loaded onto wet-spun starch-polycaprolactone scaffolds enhance bone regeneration: nude mice calvarial defect in vivo study.
Pedro P. Carvalho (2014)
Production of Clinical Grade Mesenchymal Stromal Cells
Chondrogenic potential of injectable κ-carrageenan hydrogel with encapsulated adipose stem cells for cartilage tissue-engineering applications.
Elena Geta Popa (2015)
The Current State of Clinical Cell Transplantation Trials in Iran: A Survey in 2011
Babak Arjmand (2013)
Cell manufacturing for clinical applications.
Babak Arjmand (2014)
ADIPOSE-DERIVED STEM CELLS IN TISSUE ENGINEERING: LABORATORY TO BEDSIDE
Ahmad Sukari Halim (2013)
Comparison of Stromal/Stem Cells Isolated from Human Omental and Subcutaneous Adipose Depots: Differentiation and Immunophenotypic Characterization
Forum S Shah (2014)
Real-time characterisation of the harvesting process for adherent mesenchymal stem cell cultures based on on-line imaging and model-based monitoring
Stefano Viazzi (2015)
Vocal Fold Lipo Injection : New Potentials from Regenerative Medicine
Leandro Batista Ribeiro (2015)
Xenofree enzymatic products for the isolation of human adipose-derived stromal/stem cells.
Pedro P. Carvalho (2013)
Isolation, expansion and characterization of bone marrow-derived mesenchymal stromal cells in serum-free conditions
Sanjay Gottipamula (2013)
Mesenchymal Stem Cells for Regenerative Therapy: Optimization of Cell Preparation Protocols
Chiho Ikebe (2014)
Comparison of dissociation capability of papain (Carica papaya L.) and trypsin on rat bone marrow mesenchymal stem cells (rBMMSCs) culture (preliminary study)
Ariyani Noviantari (2019)
Concise review: Adipose-derived stromal vascular fraction cells and stem cells: let's not get lost in translation.
Jeffrey M. Gimble (2011)
TrypZean™: An Animal-Free Alternative to Bovine Trypsin
Aparna Krishnan (2014)
Optimisation de la production par génie tissulaire de tissus humains reconstruits in vitro pour une application clinique
Catherine Henry de Villeneuve (2013)
Regenerative Therapy Using Blood-Derived Stem Cells
David S. Allan (2012)
Mesenchymal Stromal Cell Proliferation, Gene Expression and Protein Production in Human Platelet-Rich Plasma-Supplemented Media
Paola Romina Amable (2014)
Animal Protein–Free Expansion of Human Mesenchymal Stem/Progenitor Cells
Katharina Schallmoser (2012)
Human adipose-derived stromal/stem cell isolation, culture, and osteogenic differentiation.
Ammar T. Qureshi (2014)
GMP-Compliant Production of Human Placenta-Derived Mesenchymal Stem Cells.
Hamid Reza Aghayan (2020)
Clinical Grade Production of Mesenchymal Stromal Cells
Jessica J. Alm (2014)
Cryopreserved Adipose Tissue-Derived Stromal/Stem Cells: Potential for Applications in Clinic and Therapy.
Fabiana Cristina Zanata (2016)
Magneto-mechanical actuation of magnetic responsive fibrous scaffolds boosts tenogenesis of human adipose stem cells.
Ana Rita Tomás (2019)
The Anti-Tumor Effects of Adipose Tissue Mesenchymal Stem Cell Transduced with HSV-Tk Gene on U-87-Driven Brain Tumor
Suely Maymone de Melo (2015)
The Relative Functionality of Freshly Isolated and Cryopreserved Human Adipose-Derived Stromal/Stem Cells
Forum S Shah (2016)
Development of a Perfusion Bioreactor Strategy for Human Adipose-Derived Stem Cell Expansion
Sarah Alicia-Ann Fleming (2011)