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Feeding Barley Grain-rich Diets Altered Electrophysiological Properties And Permeability Of The Ruminal Wall In A Goat Model.

Fenja Klevenhusen, Manfred Hollmann, L Podstatzky-Lichtenstein, Reinhild Krametter-Froetscher, Jörg R Aschenbach, Qendrim Zebeli
Published 2013 · Medicine, Biology
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High-producing ruminants are commonly fed large amounts of concentrate to meet their high energy demands for rapid growth or high milk production. However, this feeding strategy can severely impair rumen functioning, leading to subacute ruminal acidosis. Subacute ruminal acidosis might have consequences for electrophysiological properties by changing the net ion transfer and permeability of ruminal epithelia, which may increase the uptake of toxic compounds generated in the rumen into the systemic circulation. The objective of the present study was to investigate the effects of excessive barley feeding on the electrophysiological and barrier functions of the ruminal epithelium and serum inflammation and ketogenesis markers after a long-term feeding challenge, using growing goats as a ruminant model. A feeding trial was carried out with growing goats allocated to 1 of the 3 groups (n=5-6 animals/group), with diets consisting exclusively of hay (control diet) or hay with 30 or 60% barley grain. Samples of the ventral ruminal epithelium were taken after euthanasia and instantly subjected to Ussing chamber experiments, where electrophysiological properties of the epithelium were measured in parallel with the permeability of marker molecules of different sizes [fluorescein 5(6)-isothiocyanate and horseradish peroxidase] from luminal to apical side. Additionally, ruminal fluid and blood samples were taken at the beginning of the experiment as well as shortly before euthanasia. Ruminal fluid samples were analyzed for volatile fatty acids and pH, whereas blood samples were analyzed for lipopolysaccharide, serum amyloid A, and β-hydroxybutyrate. Electrophysiological data indicated that barley feeding increased the epithelial short-circuit current compared with the control. Tissue conductance also increased with dietary barley inclusion. As shown with both marker molecules, permeability of ruminal epithelia increased with barley inclusion in the diet. Despite a lowered ruminal pH associated with increased volatile fatty acids (such as propionate and butyrate) concentrations as well as altered epithelial properties in response to high-grain feeding, no signs of inflammation became apparent, as blood serum amyloid A concentrations remained unaffected by diet. However, greater amounts of grain in the diet were associated with a quadratic increase in lipopolysaccharide concentration in the serum. Also, increasing the amounts of barley grain in the diet resulted in a tendency to quadratically augment serum concentrations of β-hydroxybutyrate and, hence, the alimentary ketogenesis. Further studies are needed to clarify the role of barley inclusion in the development of subacute ruminal acidosis in relation to ruminal epithelial damage and the translocation of toxic compounds in vivo.
This paper references
10.1016/S0921-4488(98)00116-3
Experimentally induced lactic acidosis in Nubian goats: Clinical, biochemical and pathological investigations
M S Nour (1998)
10.1113/expphysiol.1987.sp003092
The effect of diet, intraruminal pH and osmolarity on sodium, chloride and magnesium absorption from the temporarily isolated and washed reticulo-rumen of sheep.
Gotthold Gaebel (1987)
10.1111/j.1439-0442.1991.tb01043.x
Influences of diet, short-chain fatty acids, lactate and chloride on bicarbonate movement across the reticulo-rumen wall of sheep.
Gotthold Gaebel (1991)
10.1136/gut.45.6.879
Comparison of cattle and sheep colonic permeabilities to horseradish peroxidase and hamster scrapie prion protein in vitro
Andrew T. Mckie (1999)
10.3168/jds.2007-0572
Modeling the adequacy of dietary fiber in dairy cows based on the responses of ruminal pH and milk fat production to composition of the diet.
Q. Zebeli (2008)
perimentally induced lactic acidosis in Nubian goats : Clinical , biochemical and pathological investigations
M. McGee (1998)
10.2527/jas.2010-3378
Ruminant Nutrition Symposium: Molecular adaptation of ruminal epithelia to highly fermentable diets.
G Penner (2011)
10.1007/s11306-010-0227-6
Metabolomics reveals unhealthy alterations in rumen metabolism with increased proportion of cereal grain in the diet of dairy cows
B. Ametaj (2010)
10.1111/j.1749-6632.2012.06553.x
Microbial butyrate and its role for barrier function in the gastrointestinal tract.
Svenja Plöger (2012)
10.1016/j.rvsc.2012.02.004
Interplay between rumen digestive disorders and diet-induced inflammation in dairy cattle.
Qendrim Zebeli (2012)
10.1079/AHRR200237
Transfer of energy substrates across the ruminal epithelium: implications and limitations.
G. Gaebel (2002)
10.1016/J.ANIFEEDSCI.2011.12.004
Subacute ruminal acidosis (SARA), endotoxins and health consequences
Jan C. Plaizier (2012)
Recommendations for the Supply of Energy and Nutrients to Goats
W. Drochner (2004)
10.1590/S1516-35982010001300048
Nutrition, microbiota, and endotoxin-related diseases in dairy cows
B. Ametaj (2010)
10.3168/jds.S0022-0302(06)72487-0
Rumen lipopolysaccharide and inflammation during grain adaptation and subacute ruminal acidosis in steers.
G. Gozho (2006)
Evaluation of health and ruminal variables during adaptation to grain-based diets in beef cattle.
Jane A. Z. Leedle (1995)
10.3168/jds.2011-4447
Effects of subacute ruminal acidosis challenges on fermentation and endotoxins in the rumen and hindgut of dairy cows.
S. Li (2012)
10.2527/2000.7882223x
Portal-drained visceral metabolism of 3-hydroxybutyrate in sheep.
Niels Bastian Kristensen (2000)
10.15446/rfmvz
Revista de la Facultad de Medicina Veterinaria y de Zootecnia
Revista de la Facultad de Medicina Veter Fmvz (1952)
Haptoglobin and serum amyloid a in subacute ruminal acidosis in goats
F. D. González (2010)
10.2527/jas.2010-3301
Ruminant Nutrition Symposium: Role of fermentation acid absorption in the regulation of ruminal pH.
Jörg R Aschenbach (2011)
10.3168/jds.2008-1389
A grain-based subacute ruminal acidosis challenge causes translocation of lipopolysaccharide and triggers inflammation.
E. Khafipour (2009)
10.3168/JDS.2007-0257
Acidosis and lipopolysaccharide from Escherichia coli B:055 cause hyperpermeability of rumen and colon tissues.
D. G. Emmanuel (2007)
10.1186/1751-0147-51-39
Ruminal acidosis and the rapid onset of ruminal parakeratosis in a mature dairy cow: a case report
Michael A Steele (2009)
10.1046/j.1439-0442.2003.00569.x
Subacute ruminal acidosis (SARA): a review.
J. Kleen (2003)
10.3168/jds.2010-3406
A single mild episode of subacute ruminal acidosis does not affect ruminal barrier function in the short term.
Gregory B. Penner (2010)
10.1016/j.smallrumres.2003.08.003
Recommendations for the Supply of Energy and Nutrients to Goats
George F.W. Haenlein (2004)
10.3168/jds.2007-0256
Feeding high proportions of barley grain stimulates an inflammatory response in dairy cows.
D. G. Emmanuel (2008)
10.3168/jds.2010-3860
Perturbations of plasma metabolites correlated with the rise of rumen endotoxin in dairy cows fed diets rich in easily degradable carbohydrates.
Q. Zebeli (2011)
10.1042/bj3340489
Regulation of serum amyloid A protein expression during the acute-phase response.
L. Jensen (1998)
10.3168/jds.S0022-0302(91)78551-2
Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition.
P. V. Van Soest (1991)
10.2527/jas.2007-0178
Rumen fermentation, microbial protein synthesis, and nutrient flow to the omasum in cattle offered corn silage, grass silage, or whole-crop wheat.
D. Owens (2009)
10.2527/2000.782464x
Effect and absorption of histamine in sheep rumen: significance of acidotic epithelial damage.
J. Aschenbach (2000)
10.3168/jds.2008-1465
Feeding high proportions of barley grain in a total mixed ration perturbs diurnal patterns of plasma metabolites in lactating dairy cows.
B. Ametaj (2009)
10.1152/ajpregu.00120.2010
Bovine rumen epithelium undergoes rapid structural adaptations during grain-induced subacute ruminal acidosis.
Michael A Steele (2011)
10.3168/jds.2008-1656
Alfalfa pellet-induced subacute ruminal acidosis in dairy cows increases bacterial endotoxin in the rumen without causing inflammation.
E. Khafipour (2009)



This paper is referenced by
10.1016/j.rvsc.2018.08.005
Diet-induced inflammation: From gut to metabolic organs and the consequences for the health and longevity of ruminants.
Ratchaneewan Khiaosa-Ard (2018)
10.1152/ajpregu.00035.2014
Short-term adaptation of the ruminal epithelium involves abrupt changes in sodium and short-chain fatty acid transport.
B. L. Schurmann (2014)
EFFECT OF ERGOT ALKALOIDS ON BOVINE FOREGUT VASCULATURE, NUTRIENT ABSORPTION, AND EPITHELIAL BARRIER FUNCTION
Andrew P. Foote (2013)
10.1186/s12917-018-1705-8
Replacement of grains with soybean hulls ameliorates SARA-induced impairment of the colonic epithelium barrier function of goats
K. Zhang (2018)
10.1186/s40104-016-0100-1
High-concentrate feeding upregulates the expression of inflammation-related genes in the ruminal epithelium of dairy cattle
Ruiyang Zhang (2016)
10.1017/s0022029920000060
Rumen function in goats, an example of adaptive capacity
S Giger-Reverdin (2020)
FUNCTIONAL ADAPTATION OF THE RUMINAL EPITHELIUM
B. L. Schurmann (2013)
10.1017/S1751731115002888
Relative contribution of ruminal buffering systems to pH regulation in feedlot cattle fed either low- or high-forage diets.
G. E. Chibisa (2016)
10.1017/s0022029920000369
Ruminal epithelium: a checkpoint for cattle health.
Lisa Baaske (2020)
10.1017/S1751731114001128
High concentrate-induced subacute ruminal acidosis (SARA) increases plasma acute phase proteins (APPs) and cortisol in goats.
Yimin Jia (2014)
10.1152/ajpregu.00068.2013
A high-grain diet causes massive disruption of ruminal epithelial tight junctions in goats.
Jun-hua Liu (2013)
10.1016/J.LIVSCI.2014.01.005
Cattle's variation in rumen ecology and metabolism and its contributions to feed efficiency
Ratchaneewan Khiaosa-Ard (2014)
10.3168/jds.2018-15243
Symposium review: The importance of the ruminal epithelial barrier for a healthy and productive cow.
Jörg R Aschenbach (2019)
10.1093/jas/skx017
Effect of individual SCFA on the epithelial barrier of sheep rumen under physiological and acidotic luminal pH conditions.
Gabriele Greco (2018)
10.3168/jds.2014-9166
Pyrosequencing reveals shifts in the bacterial epimural community relative to dietary concentrate amount in goats.
S. U. Wetzels (2015)
10.1007/s10096-019-03549-4
Targeting gut microbiota as a possible therapy for mastitis
Xiaoyu Hu (2019)
10.1016/J.ANIFEEDSCI.2016.06.009
Both monensin and plant extract alter ruminal fermentation in sheep but only monensin affects the expression of genes involved in acid-base transport of the ruminal epithelium
H. Mirzaei-Alamouti (2016)
10.1016/J.SMALLRUMRES.2018.09.017
The effects of subacute ruminal acidosis on rumen epithelium barrier function in dairy goats
Yu Ying Sun (2018)
10.3168/jds.2016-11208
Metabolic responses, performance, and reticuloruminal pH of early-lactating cows fed concentrates treated with lactic acid, with or without inorganic phosphorus supplementation.
Annabella Khol-Parisini (2016)
10.3168/jds.2015-9522
Long-term reticuloruminal pH dynamics and markers of liver health in early-lactating cows of various parities fed diets differing in grain processing.
Elke Humer (2015)
A high-grain diet causes massive disruption of ruminal epithelial tight 1 junctions in goats 2 3
Jun-hua Liu (2013)
10.1111/jpn.12296
Stimulating effects of a diet negative in dietary cation-anion difference on calcium absorption from the rumen in sheep.
M R Wilkens (2016)
10.1155/2016/9618795
Arginine Relieves the Inflammatory Response and Enhances the Casein Expression in Bovine Mammary Epithelial Cells Induced by Lipopolysaccharide
T. Wu (2016)
10.3168/jds.2016-12262
Key role of short-chain fatty acids in epithelial barrier failure during ruminal acidosis.
Svenja Meissner (2017)
10.1016/j.tvjl.2014.03.025
A high-grain diet alters the omasal epithelial structure and expression of tight junction proteins in a goat model.
Junhua Liu (2014)
BARLEY GRAIN-FEED INDUSTRY GUIDE 2 FEED BARLEY BACKGROUND & MARKET
Sarah J Meale (2015)
10.1017/S1751731118001064
Feeding corn grain steeped in citric acid modulates rumen fermentation and inflammatory responses in dairy goats.
Y. Shen (2019)
10.2527/jas.2013-6729
Assessment of gastrointestinal permeability by lactulose test in sheep after repeated indomethacin treatment.
Andrea Minuti (2013)
Studies of the responses of cattle and sheep to rapidly fermentable carbohydrate challenges
Holly J. Ferguson (2018)
10.1371/journal.pone.0081602
Downregulation of Cellular Protective Factors of Rumen Epithelium in Goats Fed High Energy Diet
Manfred Hollmann (2013)
10.1371/journal.pone.0111596
High Concentrate Diet Induced Mucosal Injuries by Enhancing Epithelial Apoptosis and Inflammatory Response in the Hindgut of Goats
Shiyu Tao (2014)
10.1017/S0007114514000993
High-grain feeding alters caecal bacterial microbiota composition and fermentation and results in caecal mucosal injury in goats.
Junhua Liu (2014)
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