The Difference Of Physiological And Proteomic Changes In Maize Leaves Adaptation To Drought, Heat, And Combined Both Stresses
Feiyun Zhao, D. Zhang, Y. Zhao, Wei Wang, Hao Yang, F. Tai, C. Li, Xiuli Hu
Published 2016 · Biology, Medicine
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At the eight-leaf stage, maize is highly sensitive to stresses such as drought, heat, and their combination, which greatly affect its yield. At present, few studies have analyzed maize response to combined drought and heat stress at the eight-leaf stage. In this study, we measured certain physical parameters of maize at the eight-leaf stage when it was exposed to drought, heat, and their combination. The results showed an increase in the content of H2O2 and malondialdehyde (MDA), and in the enzyme activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), and glutathione reductase (GR), but a decrease in the quantum efficiency of photosystem II (ΦPSII). The most obvious increase or decrease in physical parameters was found under the combined stress condition. Moreover, to identify proteins differentially regulated by the three stress conditions at the eight-leaf stage, total proteins from the maize leaves were identified and quantified using multiplex iTRAQ-based quantitative proteomic and LC-MS/MS methods. In summary, the expression levels of 135, 65, and 201 proteins were significantly changed under the heat, drought and combined stress conditions, respectively. Of the 135, 65, and 201 differentially expressed proteins, 61, 28, and 16 responded exclusively to drought stress, heat stress, and combined stress, respectively. Bioinformatics analysis implied that chaperone proteins and proteases play important roles in the adaptive response of maize to heat stress and combined stress, and that the leaf senescence promoted by ethylene-responsive protein and ripening-related protein may play active roles in maize tolerance to combined drought and heat stress. The signaling pathways related to differentially expressed proteins were obviously different under all three stress conditions. Thus, the functional characterization of these differentially expressed proteins will be helpful for discovering new targets to enhance maize tolerance to stress.
This paper references
The maize (Zea mays
L. Andrés-Hernández (2015)
Role of plant
W. Wang (2004)
Quantitative proteomics reveals the role of protein phosphorylation in rice embryos during early stages of germination.
Chao Han (2014)
Abiotic and biotic stress combinations.
N. Suzuki (2014)
Small heat shock proteins: recent developments
Benjamin D Eisenhardt (2013)
The Unfolded Protein Response and the Role of Protein Disulfide Isomerase in Neurodegeneration
E. Perri (2016)
Ethylene production and senescence
S. 1007s00344-010-9157-9 Jana (1982)
Going beyond nutrition
U. Anschütz (2014)
Temporal transcriptome profiling reveals expression partitioning of homeologous genes contributing to heat and drought acclimation in wheat (Triticum aestivum L.)
Zhenshan Liu (2015)
Characterization of Small Heat Shock Proteins Associated with Maize Tolerance to Combined Drought and Heat Stress
Xiuli Hu (2010)
Heat and water stress induce unique transcriptional signatures of heat-shock proteins and transcription factors in grapevine
M. Rocheta (2013)
Response of chloroplast
J. Essemine (2016)
Universal sample preparation method for proteome analysis
J. Wisniewski (2009)
Overexpression of a Plasma Membrane Aquaporin in Transgenic Tobacco Improves Plant Vigor under Favorable Growth Conditions but Not under Drought or Salt Stress Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.009225.
R. Aharon (2003)
Delayed leaf senescence induces extreme drought tolerance in crop plants
Eduardo Blumwald (2009)
The regulation of protein phosphorylation.
L. Johnson (2009)
A plastid-localized glycogen synthase kinase 3 modulates stress tolerance and carbohydrate metabolism
S. Kempa (2007)
Leaf Proteome Analysis Reveals Prospective Drought and Heat Stress Response Mechanisms in Soybean
Aayudh Das (2016)
Localization of hydrogen peroxide accumulation during the hypersensitive reaction of lettuce cells to Pseudomonas syringae pv phaseolicola.
C. Bestwick (1997)
Climate Trends and Global Crop Production Since 1980
D. Lobell (2011)
Delayed leaf senescence induces extreme drought tolerance in a flowering plant
R. M. Rivero (2007)
The maize (Zea mays ssp. mays var. B73) genome encodes 33 members of the purple acid phosphatase family
Eliécer González-Muñoz (2015)
iTRAQ-based quantitative proteomic analysis reveals proteomic changes in leaves of cultivated tobacco (Nicotiana tabacum) in response to drought stress.
H. Xie (2016)
Phosphoproteome analysis reveals new drought response and defense mechanisms of seedling leaves in bread wheat (Triticum aestivum L.).
M. Zhang (2014)
2009).The regulation of protein phosphorylation
L. N. Johnson (2009)
Effect of temperature rise on crop growth and productivity
G. Rasul (2011)
Mode of action of abscisic acid in triggering ethylene biosynthesis and softening during ripening in mango fruit
S. Zaharah (2013)
Plant Mol. Biol
Chloroplast and mitochondrial proteases
A Manuell (2001)
Ethylene production and senescence in submerged aquatic angiosperms
S. Jana (1982)
The F - box protein OsFBK 12 targets OsSAMS 1 for degradation and affects pleiotropic phenotypes , including leaf senescence , in rice
Y. Chen (2013)
M. Linscheid (2005)
Characterization of maize inbred lines for drought and heat tolerance
J. Chen (2012)
Going beyond nutrition: regulation of potassium homoeostasis as a common denominator of plant adaptive responses to environment.
Uta Anschütz (2014)
T-DNA tagged knockout mutation of rice OsGSK1, an orthologue of Arabidopsis BIN2, with enhanced tolerance to various abiotic stresses
S. Koh (2007)
Response of Chloroplast NAD(P)H Dehydrogenase-Mediated Cyclic Electron Flow to a Shortage or Lack in Ferredoxin-Quinone Oxidoreductase-Dependent Pathway in Rice Following Short-Term Heat Stress
J. Essemine (2016)
Metabolite Profiles of Maize Leaves in Drought, Heat, and Combined Stress Field Trials Reveal the Relationship between Metabolism and Grain Yield1[OPEN]
Toshihiro Obata (2015)
The Variegated Mutants Lacking Chloroplastic FtsHs Are Defective in D1 Degradation and Accumulate Reactive Oxygen Species1[W][OA]
Y. Kato (2009)
Novel durum wheat genes up-regulated in response to a combination of heat and drought stress.
P. Rampino (2012)
P. O. Lim (2007)
The F-Box Protein OsFBK12 Targets OsSAMS1 for Degradation and Affects Pleiotropic Phenotypes, Including Leaf Senescence, in Rice1[W][OPEN]
Y. Chen (2013)
Comparative quantitative proteomics analysis of the ABA response of roots of drought-sensitive and drought-tolerant wheat varieties identifies proteomic signatures of drought adaptability.
S. Alvarez (2014)
Nonlinear heat effects on African maize as evidenced by historical yield trials
D. Lobell (2011)
Constitutive over-expression of AtGSK1 induces NaCl stress responses in the absence of NaCl stress and results in enhanced NaCl tolerance in Arabidopsis.
H. Piao (2001)
Leaf proteome alterations in the context of physiological and morphological responses to drought and heat stress in barley (Hordeum vulgare L.)
J. Rollins (2013)
Integrative network analysis of the signaling cascades in seedling leaves of bread wheat by large-scale phosphoproteomic profiling.
Dong-Wen Lv (2014)
Transcriptomic analysis of Sorghum bicolor responding to combined heat and drought stress
S. M. Johnson (2013)
The Fbox protein OsFBK12 targets OsSAMS1 for degradation and affects pleiotropic phenotypes, including leaf senescence, in rice
Y Chen (2013)
No use, distribution or reproduction is permitted which does not comply with these terms
Role of phosphatidic
W. W. Xu (2010)
ABA receptor PYL9 promotes drought resistance and leaf senescence
Y. Zhao (2016)
Regulation of potassium transport in plants under hostile conditions: implications for abiotic and biotic stress tolerance.
S. Shabala (2014)
[Ananlysis of phosphoproteins and signalling pathways by quantitative proteomics].
Mariana Pjechová (2014)
Y. Kato (2009)
Overexpression of the Vacuolar Sugar Carrier AtSWEET16 Modifies Germination, Growth, and Stress Tolerance in Arabidopsis1[W]
P. A. Klemens (2013)
Protein disulfide isomerase: the structure of oxidative folding.
C. Gruber (2006)
Die and let live: leaf senescence contributes to plant survival under drought stress.
S. Munné-Bosch (2004)
Regulation of protein turnover by heat shock proteins.
Perinur Bozaykut (2014)
Chloroplast and mitochondrial proteases in Arabidopsis. A proposed nomenclature.
Z. Adam (2001)
Overexpression of Small Heat Shock Protein LimHSP16.45 in Arabidopsis Enhances Tolerance to Abiotic Stresses
Changjun Mu (2013)
Characterization of common and distinctive adjustments of wild barley leaf proteome under drought acclimation, heat stress and their combination
A. Ashoub (2015)
Quantitative phosphoproteomics identifies SnRK2 protein kinase substrates and reveals the effectors of abscisic acid action
P. Wang (2013)
Stress-induced electrolyte leakage: the role of K+-permeable channels and involvement in programmed cell death and metabolic adjustment.
V. Demidchik (2014)
Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds
D. Mark Hodges (1999)
The mechanisms of brassinosteroids' action: from signal transduction to plant development.
C. Yang (2011)
Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response.
W. Wang (2004)
Role of Phosphatidic Acid in High Temperature Tolerance in Maize
J. Chen (2010)
Leaf Fructose Content Is Controlled by the Vacuolar Transporter SWEET17 in Arabidopsis
F. Chardon (2013)
Eliminating the purple acid phosphatase AtPAP26 in Arabidopsis thaliana delays leaf senescence and impairs phosphorus remobilization.
W. D. Robinson (2012)
Phosphoproteomic analysis of the response of maize leaves to drought, heat and their combination stress
Xiuli Hu (2015)
A plastidlocalized glycogen synthase kinase 3 modulates stress tolerance and carbohydrate metabolism
S. Kempa (2007)
D. B. Lobell (2011)
Stress-Induced GSK3 Regulates the Redox Stress Response by Phosphorylating Glucose-6-Phosphate Dehydrogenase in Arabidopsis[C][W][OA]
Silvia Dal Santo (2012)
BRI1-Associated Receptor Kinase 1 Regulates Guard Cell ABA Signaling Mediated by Open Stomata 1 in Arabidopsis.
Y. Shang (2016)
The Kelch repeat protein KLHDC10 regulates oxidative stress-induced ASK1 activation by suppressing PP5.
Yusuke Sekine (2012)
Stress - induced GSK 3 regulates the redox stress response by phosphorylating glucose6 - phosphate dehydrogenase in Arabidopsis
S. D. Dal Santo (2012)
an aquaporin involved in radial water movement in both water uptake and transportation, altered the drought and salt tolerance of transgenic Arabidopsis
This is an Open Access article distribut...
Functional insights of plant GSK3-like kinases: multi-taskers in diverse cellular signal transduction pathways.
Ji-Hyun Youn (2015)
MzPIP 2 ; 1 : an aquaporin involved in radial water movement in both water uptake and transportation , altered the drought and salt tolerance of transgenic Arabidopsis
L. Wang (2015)
Differential expression of K+ channels between guard cells and subsidiary cells within the maize stomatal complex
Kai Büchsenschütz (2005)
Abscisic acid-induced apoplastic H2O2 accumulation up-regulates the activities of chloroplastic and cytosolic antioxidant enzymes in maize leaves
Xiuli Hu (2005)
MzPIP2;1: An Aquaporin Involved in Radial Water Movement in Both Water Uptake and Transportation, Altered the Drought and Salt Tolerance of Transgenic Arabidopsis
L. Wang (2015)
Function and evolution of 'green' GSK3/Shaggy-like kinases.
Y. Saidi (2012)
This paper is referenced by
Acquired tolerance of the photosynthetic apparatus to photoinhibition as a result of growing Solanum lycopersicum at moderately higher temperature and light intensity.
Milena Gerganova (2019)
Identification of Two Novel Wheat Drought Tolerance-Related Proteins by Comparative Proteomic Analysis Combined with Virus-Induced Gene Silencing
Xinbo Wang (2018)
Heat-Responsive Proteomics of a Heat-Sensitive Spinach Variety
S. Li (2019)
iTRAQ-Based Quantitative Analysis of Responsive Proteins Under PEG-Induced Drought Stress in Wheat Leaves
Yajing Wang (2019)
Phytochrome-Dependent Temperature Perception Modulates Isoprenoid Metabolism1
R. Bianchetti (2020)
Knock-down the expression of TaH2B-7D using virus-induced gene silencing reduces wheat drought tolerance
X. Wang (2019)
Osmotic stress at membrane level and photosystem II activity in two C4 plants after growth in elevated CO2 and temperature
Leandra Bordignon (2019)
Differential proteomic analysis reveals sequential heat stress-responsive regulatory network in radish (Raphanus sativus L.) taproot
Ronghua Wang (2018)
Enhanced Tolerance of Chinese Cabbage Seedlings Mediated by Bacillus aryabhattai H26-2 and B. siamensis H30-3 against High Temperature Stress and Fungal Infections
Y. H. Lee (2018)
The Role of the Plant Antioxidant System in Drought Tolerance
M. Laxa (2019)
Tomato proteomics: Tomato as a model for crop proteomics
Débora Vieira Parrine Sant’Ana (2018)
Genetic interaction and inheritance of biochemical traits can predict tolerance of hybrid maize cv. SC704 to drought
Mozhgan Shirinpour (2020)
Proteomic changes may lead to yield alteration in maize under carbon dioxide enriched condition
Vivek Kumar Maurya (2020)
Influence of drought and heat stress, applied independently or in combination during seed development, on qualitative and quantitative aspects of seeds of lentil (Lens culinaris Medikus) genotypes, differing in drought sensitivity.
Akanksha Sehgal (2019)
Genomics-Enabled Next-Generation Breeding Approaches for Developing System-Specific Drought Tolerant Hybrids in Maize
T. Nepolean (2018)
Comparative Proteomic and Physiological Analyses of Two Divergent Maize Inbred Lines Provide More Insights into Drought-Stress Tolerance Mechanisms
Tinashe Zenda (2018)
Proteomics, physiological, and biochemical analysis of cross tolerance mechanisms in response to heat and water stresses in soybean
Ramesh Katam (2020)
Molecular Mechanisms Associated with Drought and Heat Tolerance in Plants and Options for Crop Improvement for Combined Stress Tolerance
Makke Siva Parvathi (2020)
Algerian maize populations from the Sahara desert as potential sources of drought tolerance
A. Djemel (2019)
Proteomics and Phosphoproteomics of Heat Stress-Responsive Mechanisms in Spinach
Qi Zhao (2018)
Identification of drought tolerant populations at multi-stage growth phases in temperate maize germplasm
Abderahmane Djemel (2018)
Global warming: Antioxidant responses to deal with drought and elevated temperature in Stylosanthes capitata, a forage legume
Ricardo Borjas-Ventura (2020)
iTRAQ and virus-induced gene silencing revealed three proteins involved in cold response in bread wheat
Ning Zhang (2017)
The role of alpha-tocopherol in the protection of tomato plants against abiotic stress
Livia Spicher (2017)
Difference of proteomics vernalization-induced in bolting and flowering transitions of Beta vulgaris.
Naiguo Liang (2018)
Modulation of Abiotic Stress Tolerance Through Hydrogen Peroxide
Murat Dikilitaş (2020)
Understanding Plant Responses to Drought and Salt Stresses: Advances and Challenges in “Omics” Approaches
Mohammad Sayyar Khan (2019)
Interactive effects of drought and heat stresses on morpho-physiological attributes, yield, nutrient uptake and oxidative status in maize hybrids
H. Hussain (2019)
Molecular responses of sorghum cell suspension cultures to high temperature stress
Mamosa Gloria Ngcala (2018)
Transcriptional profiling of sugarcane leaves and roots under progressive osmotic stress reveals a regulated coordination of gene expression in a spatiotemporal manner
Alejandro Pereira-Santana (2017)
Impact on physiology and malting quality of barley exposed to heat, drought and their combination during different growth stages under controlled environment.
Ramamurthy Mahalingam (2019)
Essential role for phytol kinase and tocopherol in tolerance to combined light and temperature stress in tomato
Livia Spicher (2017)See more