Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Physico-Chemical Properties And In Vitro Antifungal Evaluation Of Samarium Doped Hydroxyapatite Coatings

Steluta Carmen Ciobanu, S. Iconaru, D. Predoi, A. Prodan, M. Predoi
Published 2020 · Materials Science

Save to my Library
Download PDF
Analyze on Scholarcy
Share
Hydroxyapatite (HAp) and samarium doped hydroxyapatite, Ca10−xSmx(PO4)6(OH)2, xSm = 0.05, (5SmHAp), coatings were prepared by sol-gel process using the dip coating method. The stability of 5SmHAp suspension was evaluated by ultrasound measurements. Fourier transform infrared spectroscopy (FTIR) was used to examine the optical characteristics of HAp and 5SmHAp nanoparticles in suspension and coatings. The FTIR analysis revealed the presence of the functional groups specific to the structure of hydroxyapatite in the 5SmHAp suspensions and coatings. The morphology of 5SmHAp nanoparticles in suspension was evaluated by transmission electron microscopy (TEM). Moreover, scanning electron microscope (SEM) was used to evaluate the morphology of nanoparticle in suspension and the morphology of the surface on the coating. The SEM and TEM studies on 5SmHAp nanoparticles in suspension showed that our samples consist of nanometric particles with elongated morphology. The SEM micrographs of HAp and 5SmHAp coatings pointed out that the coatings are continuous and homogeneous. The surface morphology of the 5SmHAp coatings was also assessed by Atomic Force Microscopy (AFM) studies. The AFM results emphasized that the coatings presented the morphology of a uniformly deposited layer with no cracks and fissures. The crystal structure of 5SmHAp coating was characterized by X-ray diffraction (XRD). The surface composition of 5SmHAp coating was analyzed by X-ray photoelectron spectroscopy (XPS). The XRD and XPS analysis shown that the Sm3+ ions have been incorporated into the 5SmHAp synthesized material. The antifungal properties of the 5SmHAp suspensions and coatings were studied using Candida albicans ATCC 10231 (C. albicans) fungal strains. The quantitative results of the antifungal assay showed that colony forming unity development was inhibited from the early phase of adherence in the case of both suspensions and coatings. Furthermore, the adhesion, cell proliferation and biofilm formation of the C. albicans were also investigated by AFM, SEM and Confocal Laser Scanning Microscopy (CLSM) techniques. The results highlighted that the C. albicans adhesion and cell development was inhibited by the 5SmHAp coatings. Moreover, the data also revealed that the 5SmHAp coatings were effective in stopping the biofilm formation on their surface. The toxicity of the 5SmHap was also investigated in vitro using HeLa cell line.
This paper references
10.3390/molecules22040604
Structural Characterization and Antifungal Studies of Zinc-Doped Hydroxyapatite Coatings
S. Iconaru (2017)
10.1063/1.357277
Surface chemistry of tin oxide based gas sensors
G. Gaggiotti (1994)
10.1016/J.COSSMS.2003.10.009
Nature-inspired calcium phosphate coatings : present status and novel advances in the science of mimicry
Ana Oliveira (2003)
10.1097/00003086-200202000-00009
Properties of osteoconductive biomaterials: calcium phosphates.
R. Legeros (2002)
10.1016/J.TSF.2009.09.046
Structural and mechanical properties of titanium oxide thin films for biomedical application
I. Jouanny (2010)
10.1155/2014/780686
Sm: HAp nanopowders present antibacterial activity against enterococcus faecalis
C. Ciobanu (2014)
10.3390/nano8050291
Antimicrobial Activity of New Materials Based on Lavender and Basil Essential Oils and Hydroxyapatite
D. Predoi (2018)
10.1039/C2BM00155A
Bioinspired phosphorylcholine containing polymer films with silver nanoparticles combining antifouling and antibacterial properties.
A. Fuchs (2013)
10.5755/J01.MS.20.1.4397
Silicone Rubber and Microcrystalline Cellulose Composites with Antimicrobial Properties
V. Jankauskaitė (2014)
10.1016/J.INJURY.2005.07.029
Bone substitutes: an update.
P. Giannoudis (2005)
10.1016/S0736-0266(01)00105-X
Hydroxyapatite crystals as a local delivery system for cisplatin: adsorption and release of cisplatin in vitro
A. Barroug (2002)
10.1186/1556-276X-6-613
Structural and physical properties of antibacterial Ag-doped nano-hydroxyapatite synthesized at 100°C
C. Ciobanu (2011)
10.1021/LA00007A041
Coadsorption of dioxygen and water on the Ni(110) surface : role of O--type species in the dissociation of water
G. U. Kulkarni (1995)
10.1016/j.msec.2016.07.057
Synthesis and characterization of silver doped hydroxyapatite nanocomposite coatings and evaluation of their antibacterial and corrosion resistance properties in simulated body fluid.
M. Mirzaee (2016)
10.1021/acsabm.0c00400
Anticancer Activity of Samarium-Coated Magnesium Implants for Immunocompromised Patients
S. Kannan (2020)
10.1002/1096-9918(200011)29:11<773::AID-SIA928>3.0.CO;2-2
Surface characterization of biocompatible hydroxyapatite coatings
C. Battistoni (2000)
10.1155/2013/284285
Study on Europium-Doped Hydroxyapatite Nanoparticles by Fourier Transform Infrared Spectroscopy and Their Antimicrobial Properties
S. Iconaru (2013)
10.1155/2016/4361051
Enamel Based Composite Layers Deposited on Titanium Substrate with Antifungal Activity
S. Iconaru (2016)
10.1016/0169-4332(94)00548-6
A comparative study of the interaction of oxygen with clusters and single-crystal surfaces of nickel
C. Rao (1995)
10.3390/polym11050886
Dextran-Coated Zinc-Doped Hydroxyapatite for Biomedical Applications
D. Predoi (2019)
10.1016/j.saa.2010.08.044
Infrared spectroscopy of different phosphates structures.
W. Jastrzȩbski (2011)
10.1016/j.msec.2019.02.073
Lithium ions (Li+) and nanohydroxyapatite (nHAp) doped with Li+ enhance expression of late osteogenic markers in adipose-derived stem cells. Potential theranostic application of nHAp doped with Li+ and co-doped with europium (III) and samarium (III) ions.
M. Alicka (2019)
10.1002/PEN.24553
Physicochemical and antimicrobial properties of silver-doped hydroxyapatite collagen biocomposite
D. Predoi (2017)
10.3390/coatings10060516
Development of Cerium-Doped Hydroxyapatite Coatings with Antimicrobial Properties for Biomedical Applications
D. Predoi (2020)
10.1007/s10856-013-4971-9
Biological evaluation of alginate-based hydrogels, with antimicrobial features by Ce(III) incorporation, as vehicles for a bone substitute
D. S. Morais (2013)
10.3390/molecules25020413
Multifunctional Hydroxyapatite Coated with Arthemisia absinthium Composites
Mariana Stefania Raita (2020)
10.1177/1010428320909999
Samarium enriches antitumor activity of ZnO nanoparticles via downregulation of CXCR4 receptor and cytochrome P450
A. I. Nabeel (2020)
10.1155/2012/942801
Synthesis, structure, and luminescent properties of europium-doped hydroxyapatite nanocrystalline powders
C. S. Ciobanu (2012)
10.1016/j.ejmp.2020.06.007
Samarium doped titanium dioxide nanoparticles as theranostic agents in radiation therapy.
M. Nakayama (2020)
10.3390/nano9091295
Synthesis, Characterization, and Antimicrobial Activity of Magnesium-Doped Hydroxyapatite Suspensions
D. Predoi (2019)
10.1016/J.TSF.2008.04.071
Hydroxyapatite films obtained by sol–gel and sputtering
T. Stoica (2008)
10.1016/S0039-6028(99)00997-8
Photoemission study of dissociatively adsorbed methane on a pre-oxidized SnO2 thin film
T. Kawabe (2000)
10.1021/ACS.IECR.5B04329
Tailoring the Sm/Gd-Substituted Hydroxyapatite Coating on Biomedical AISI 316L SS: Exploration of Corrosion Resistance, Protein Profiling, Osteocompatibility, and Osteogenic Differentiation for Orthopedic Implant Applications
S. Sathishkumar (2016)
10.1002/JBM.1065
Surface and biological evaluation of hydroxyapatite-based coatings on titanium deposited by different techniques.
C. Massaro (2001)
10.1016/S0169-4332(99)00267-6
XPS study of apatite-based coatings prepared by sol-gel technique
S. Kaciulis (1999)
10.1371/journal.pone.0173118
Synthesis and characterization of porous silicon as hydroxyapatite host matrix of biomedical applications
A. Dussan (2017)
10.1002/jbm.b.31746
Nanosized hydroxyapatite and other calcium phosphates: chemistry of formation and application as drug and gene delivery agents.
V. Uskoković (2011)
10.3390/coatings9120773
Biocompatible Layers Obtained from Functionalized Iron Oxide Nanoparticles in Suspension
D. Predoi (2019)
10.1016/j.msec.2016.05.019
A doxorubicin delivery system: Samarium/mesoporous bioactive glass/alginate composite microspheres.
Y. Zhang (2016)
10.1186/1471-2334-6-130
How long do nosocomial pathogens persist on inanimate surfaces? A systematic review
A. Kramer (2006)
10.3390/s19132908
Silicon-Based Sensors for Biomedical Applications: A Review
Yongzhao Xu (2019)
10.1155/2015/849216
Evaluation of samarium doped hydroxyapatite, ceramics for medical application: antimicrobial activity
C. S. Ciobanu (2015)
10.1002/jbm.a.34755
Influence of substrate metal alloy type on the properties of hydroxyapatite coatings deposited using a novel ambient temperature deposition technique.
J. N. Barry (2014)
10.1088/1748-6041/5/5/054114
Osteoblastic cell response on fluoridated hydroxyapatite coatings: the effect of magnesium incorporation.
Y. Cai (2010)
10.1039/C5RA15815J
Band edge movement in dye sensitized Sm-doped TiO2 solar cells: a study by variable temperature spectroelectrochemistry
L. Wei (2015)
10.1016/J.SPMI.2017.06.021
Ag films deposited on Si and Ti: How the film-substrate interaction influences the nanoscale film morphology
F. Ruffino (2017)
10.2174/1877946809666190828104812
Rare Earth Doped Hydroxyapatite Nanoparticles for In Vitro Bioimaging Applications
Pranjita Zantye (2019)
10.3390/coatings10010014
Silver-Doped Hydroxyapatite Thin Layers Obtained by Sol-Gel Spin Coating Procedure
A. Prodan (2019)
10.1016/J.CERAMINT.2015.03.316
Substituted hydroxyapatites for biomedical applications: A review
Monika Šupová (2015)
10.1111/j.1600-0501.2011.02362.x
Effects of magnesium-substituted nanohydroxyapatite coating on implant osseointegration.
Shifang Zhao (2013)
10.3390/COATINGS8080276
Bioceramic Layers with Antifungal Properties
D. Predoi (2018)
10.1021/IC50131A039
Infrared studies of apatites. I. Vibrational assignments for calcium, strontium, and barium hydroxyapatites utilizing isotopic substitution
B. O. Fowler (1974)
10.1111/j.1442-9071.2007.01423.x
Contemporary in vivo confocal microscopy of the living human cornea using white light and laser scanning techniques: a major review
D. Patel (2007)
10.3390/nano9040515
Evaluation of Antibacterial Activity of Zinc-Doped Hydroxyapatite Colloids and Dispersion Stability Using Ultrasounds
D. Predoi (2019)
10.1039/C4TB00484A
Samarium doped glass-reinforced hydroxyapatite with enhanced osteoblastic performance and antibacterial properties for bone tissue regeneration.
D. Morais (2014)
10.1186/1556-276X-7-324
Antibacterial activity of silver-doped hydroxyapatite nanoparticles against gram-positive and gram-negative bacteria
C. Ciobanu (2012)
10.3390/COATINGS9030156
Zinc Doped Hydroxyapatite Thin Films Prepared by Sol–Gel Spin Coating Procedure
D. Predoi (2019)
10.1016/j.msec.2020.111238
The mechanical and chemical stability of the interfaces in bioactive materials: The substrate-bioactive surface layer and hydroxyapatite-bioactive surface layer interfaces.
S. Ferraris (2020)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar