← Back to Search
ITRAQ-Based Proteomic Analysis Reveals Several Strategies To Cope With Drought Stress In Maize Seedlings
Z. Jiang, Fengxue Jin, X. Shan, Yidan Li
Published 2019 · Biology, Medicine
Download PDFAnalyze on Scholarcy
Drought stress, especially during the seedling stage, seriously limits the growth of maize and reduces production in the northeast of China. To investigate the molecular mechanisms of drought response in maize seedlings, proteome changes were analyzed. Using an isotopic tagging relative quantitation (iTRAQ) based method, a total of 207 differentially accumulated protein species (DAPS) were identified under drought stress in maize seedlings. The DAPS were classified into ten essential groups and analyzed thoroughly, which involved in signaling, osmotic regulation, protein synthesis and turnover, reactive oxygen species (ROS) scavenging, membrane trafficking, transcription related, cell structure and cell cycle, fatty acid metabolism, carbohydrate and energy metabolism, as well as photosynthesis and photorespiration. The enhancements of ROS scavenging, osmotic regulation, protein turnover, membrane trafficking, and photosynthesis may play important roles in improving drought tolerance of maize seedlings. Besides, the inhibitions of some protein synthesis and slowdown of cell division could reduce the growth rate and avoid excessive water loss, which is possible to be the main reasons for enhancing drought avoidance of maize seedlings. The incongruence between protein and transcript levels was expectedly observed in the process of confirming iTRAQ data by quantitative real-time polymerase chain reaction (qRT-PCR) analysis, which further indicated that the multiplex post-transcriptional regulation and post-translational modification occurred in drought-stressed maize seedlings. Finally, a hypothetical strategy was proposed that maize seedlings coped with drought stress by improving drought tolerance (via. promoting osmotic adjustment and antioxidant capacity) and enhancing drought avoidance (via. reducing water loss). Our study provides valuable insight to mechanisms underlying drought response in maize seedlings.
This paper references
Enhancing Omics Research of Crop Responses to Drought under Field Conditions
S. Wu (2017)
Comparative Proteomic Analysis of Brassica napus in Response to Drought Stress.
Jin Koh (2015)
A Grape ASR Protein Involved in Sugar and Abscisic Acid Signaling Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.013854.
B. Çakır (2003)
Differential regulation of proteins and phosphoproteins in rice under drought stress.
Y. Ke (2009)
Functional photosystem I maintains proper energy balance during nitrogen depletion in Chlamydomonas reinhardtii, promoting triacylglycerol accumulation
M. Gargouri (2017)
Maize Histone Deacetylase hda101 Is Involved in Plant Development, Gene Transcription, and Sequence-Specific Modulation of Histone Modification of Genes and Repeats[W]
V. Rossi (2007)
The Plant Dehydrins: Structure and Putative Functions
Ch. R. Allagulova (2004)
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license
A pivotal regulatory module for plant growth and development
T P Sun (2010)
Analysis of histones and histone variants in plants.
Ila Trivedi (2012)
Gibberellin-GID1-DELLA: A Pivotal Regulatory Module for Plant Growth and Development1
T. Sun (2010)
Water-Deficit Tolerance and Field Performance of Transgenic Alfalfa Overexpressing Superoxide Dismutase
B. Mckersie (1996)
Over-expression of a glutathione S-transferase gene, GsGST, from wild soybean (Glycine soja) enhances drought and salt tolerance in transgenic tobacco
W. Ji (2010)
The Physiology and Proteomics of Drought Tolerance in Maize: Early Stomatal Closure as a Cause of Lower Tolerance to Short-Term Dehydration?
M. Benešová (2012)
Water deficit and growth. Co-ordinating processes without an orchestrator?
F. Tardieu (2011)
The role of gibberellin signalling in plant responses to abiotic stress
Ellen H Colebrook (2014)
Alternative Splicing Control of Abiotic Stress Responses.
Tom Laloum (2018)
Reactive oxygen species homeostasis and signalling during drought and salinity stresses.
G. Miller (2010)
Physiological and proteome analysis suggest critical roles for the photosynthetic system for high water-use efficiency under drought stress in Malus.
S. Zhou (2015)
iTRAQ-based quantitative proteomic analysis reveals new metabolic pathways responding to chilling stress in maize seedlings.
X. Wang (2016)
Proteomics techniques for the development of flood tolerant crops.
Setsuko Komatsu (2012)
Strategies for developing Green Super Rice
Q. Zhang (2007)
exit from proliferation, genome stability and plant development
A Doskocilova (2013)
Role of histone deacetylases HDA6 and HDA19 in ABA and abiotic stress response
Li-ting Chen (2010)
From Genetics to Functional Genomics: Improvement in Drought Signaling and Tolerance in Wheat
H. Budak (2015)
Characterization of the calcineurin B-Like (CBL) gene family in maize and functional analysis of ZmCBL9 under abscisic acid and abiotic stress treatments.
Fan Zhang (2016)
Identification of Drought Tolerant Mechanisms in Maize Seedlings Based on Transcriptome Analysis of Recombination Inbred Lines
Haowei Min (2016)
Transgenic Arabidopsis Plants Expressing Tomato Glutathione S-Transferase Showed Enhanced Resistance to Salt and Drought Stress
Jing Xu (2015)
Histone deacetylase HDA9 negatively regulates salt and drought stress responsiveness in Arabidopsis.
Y. Zheng (2016)
Root proteome of rice studied by iTRAQ provides integrated insight into aluminum stress tolerance mechanisms in plants.
Zhan Qi Wang (2014)
Effects of ABA, auxin, and gibberellin on the expression of genes for vacuolar H+ -inorganic pyrophosphatase, H+ -ATPase subunit A, and Na+/H+ antiporter in barley.
A. Fukuda (2006)
Molecular Breeding to Improve Plant Resistance to Abiotic Stresses
G. J. N. Rao (2016)
NITRILASE1 regulates the exit from proliferation, genome stability and plant development.
Anna Doskočilová (2013)
Proteomics applied on plant abiotic stresses: role of heat shock proteins (HSP).
A. M. Timperio (2008)
Van Der Straeten, D. Exploiting DELLA Signaling in Cereals
K Van De Velde (2017)
Comparative Proteomics Analysis of the Seedling Root Response of Drought-sensitive and Drought-tolerant Maize Varieties to Drought Stress
Wenjing Zeng (2019)
Coping with abiotic stress: proteome changes for crop improvement.
I. Abreu (2013)
Overexpression of the glutathione S-transferase gene from Pyrus pyrifolia fruit improves tolerance to abiotic stress in transgenic tobacco plants
D. Liu (2013)
Systems-based analysis of Arabidopsis leaf growth reveals adaptation to water deficit
K. Baerenfaller (2012)
Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses.
W. Wang (2009)
Genome-Wide Mapping of Targets of Maize Histone Deacetylase HDA101 Reveals Its Function and Regulatory Mechanism during Seed Development[OPEN]
H. Yang (2016)
Effect of drought stress on lipid metabolism in the leaves of Arabidopsis thaliana (ecotype Columbia).
A. Gigon (2004)
Comparative Proteomic Analysis of Soybean Leaves and Roots by iTRAQ Provides Insights into Response Mechanisms to Short-Term Salt Stress
W. Ji (2016)
Proteomics study reveals the molecular mechanisms underlying water stress tolerance induced by Piriformospora indica in barley.
M. Ghabooli (2013)
The Histone H1 Variant Accumulates in Response to Water Stress in the Drought Tolerant Genotype of Gossypium herbaceum L.
I. Trivedi (2012)
Differential proteomic analysis of drought stress response in leaves of common bean (Phaseolus vulgaris L.).
T. Zadražnik (2013)
Identification of regulatory network hubs that control lipid metabolism in Chlamydomonas reinhardtii
M. Gargouri (2015)
Overexpression of Camellia sinensis H1 histone gene confers abiotic stress tolerance in transgenic tobacco
W. Wang (2014)
Organ-specific proteomic analysis of drought-stressed soybean seedlings.
P. Mohammadi (2012)
Light-harvesting chlorophyll a/b-binding proteins are required for stomatal response to abscisic acid in Arabidopsis
Yan-Hong Xu (2012)
iTRAQ-based quantitative proteomic analysis reveals proteomic changes in leaves of cultivated tobacco (Nicotiana tabacum) in response to drought stress.
H. Xie (2016)
Redox proteomics and physiological responses in Cistus albidus shrubs subjected to long-term summer drought followed by recovery
Ricard Brossa (2014)
Expression of small heat-shock proteins at low temperatures. A possible role in protecting against chilling injuries.
A. Sabehat (1998)
The Paragon Algorithm, a Next Generation Search Engine That Uses Sequence Temperature Values and Feature Probabilities to Identify Peptides from Tandem Mass Spectra*S
Ignat V Shilov (2007)
Gibberellin biosynthesis and its regulation.
P. Hedden (2012)
Drought-Responsive Mechanisms in Plant Leaves Revealed by Proteomics
X. Wang (2016)
Increasing Confidence of Proteomics Data Regarding the Identification of Stress-Responsive Proteins in Crop Plants
X. Wu (2016)
Network Candidate Genes in Breeding for Drought Tolerant Crops
C. Krannich (2015)
Transcriptional profiling of Arabidopsis heat shock proteins and transcription factors reveals extensive overlap between heat and non-heat stress response pathways
W. Swindell (2006)
Phosphoproteome Dynamics Upon Changes in Plant Water Status Reveal Early Events Associated With Rapid Growth Adjustment in Maize Leaves*
Ludovic Bonhomme (2012)
Advances in Plant Breeding Strategies: Agronomic, Abiotic and Biotic Stress Traits
J. M. Al-Khayri (2016)
Comparative Proteomics and Physiological Analyses Reveal Important Maize Filling-Kernel Drought-Responsive Genes and Metabolic Pathways
X. Wang (2019)
Comparative Proteomic and Physiological Analyses of Two Divergent Maize Inbred Lines Provide More Insights into Drought-Stress Tolerance Mechanisms
Tinashe Zenda (2018)
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
K. Livak (2001)
iTRAQ-Based Quantitative Proteomic Analysis of Embryogenic and Non-embryogenic Calli Derived from a Maize (Zea mays L.) Inbred Line Y423
B. Liu (2018)
Quantitative Proteomic Analysis of Wheat Cultivars with Differing Drought Stress Tolerance
K. Ford (2011)
Physiological and proteomic analysis of maize seedling response to water deficiency stress.
Longfei Xin (2018)
Homologous expression of cytosolic dehydroascorbate reductase increases grain yield and biomass under paddy field conditions in transgenic rice (Oryza sativa L. japonica)
Y. Kim (2013)
Involvement of Arabidopsis histone deacetylase HDA6 in ABA and salt stress response
Li-ting Chen (2010)
Exploiting DELLA Signaling in Cereals.
Karel Van De Velde (2017)
Transcriptional regulation of the V-ATPase subunit c and V-PPase isoforms in Cucumis sativus under heavy metal stress.
Katarzyna Kabała (2014)
This paper is referenced by
Label-Free Comparative Proteomic Analysis Combined with Laser-Capture Microdissection Suggests Important Roles of Stress Responses in the Black Layer of Maize Kernels
Quanquan Chen (2020)
Integrative Transcriptomic and Proteomic Analyses of Molecular Mechanism Responding to Salt Stress during Seed Germination in Hulless Barley
Yong Lai (2020)
Adapting Cereal Grain Crops to Drought Stress: 2020 and Beyond
Tinashe Zenda (2020)