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Efficacy And Durability In Direct Labeling Of Mesenchymal Stem Cells Using Ultrasmall Superparamagnetic Iron Oxide Nanoparticles With Organosilica, Dextran, And PEG Coatings

Y. Wang, Thibault Quercy-Jouvet, Hao-Hao Wang, Ak-Wai Li, Chun-Pong Chak, Shouhu Xuan, L. Shi, Defeng Wang, Siu-Fung Lee, P. Leung, C. B. S. Lau, K. Fung, K. Leung
Published 2011 · Materials Science, Medicine

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We herein report a comparative study of mesenchymal stem cell (MSC) labeling using spherical superparamagnetic iron oxide (SPIO) nanoparticles containing different coatings, namely, organosilica, dextran, and poly(ethylene glycol) (PEG). These nanomaterials possess a similar SPIO core size of 6–7 nm. Together with their coatings, the overall sizes are 10–15 nm for all SPIO@SiO2, SPIO@dextran, and SPIO@PEG nanoparticles. These nanoparticles were investigated for their efficacies to be uptaken by rabbit bone marrow-derived MSCs without any transfecting agent. Experimentally, both SPIO@SiO2 and SPIO@PEG nanoparticles could be successfully uptaken by MSCs while the SPIO@dextran nanoparticles demonstrated limited labeling efficiency. The labeling durability of SPIO@SiO2 and SPIO@PEG nanoparticles in MSCs after three weeks of culture were compared by Prussian blue staining tests. SPIO@SiO2 nanoparticles demonstrated more blue staining than SPIO@PEG nanoparticles, rendering them better materials for MSCs labeling by direct uptake when durable intracellullar retention of SPIO is desired.
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