Online citations, reference lists, and bibliographies.
← Back to Search

Inorganic Materials As Supports For Covalent Enzyme Immobilization: Methods And Mechanisms

P. Zucca, E. Sanjust
Published 2014 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
Several inorganic materials are potentially suitable for enzymatic covalent immobilization, by means of several different techniques. Such materials must meet stringent criteria to be suitable as solid matrices: complete insolubility in water, reasonable mechanical strength and chemical resistance under the operational conditions, the capability to form manageable particles with high surface area, reactivity towards derivatizing/functionalizing agents. Non-specific protein adsorption should be always considered when planning covalent immobilization on inorganic solids. A huge mass of experimental work has shown that silica, silicates, borosilicates and aluminosilicates, alumina, titania, and other oxides, are the materials of choice when attempting enzyme immobilizations on inorganic supports. More recently, some forms of elemental carbon, silicon, and certain metals have been also proposed for certain applications. With regard to the derivatization/functionalization techniques, the use of organosilanes through silanization is undoubtedly the most studied and the most applied, although inorganic bridge formation and acylation with selected acyl halides have been deeply studied. In the present article, the most common inorganic supports for covalent immobilization of the enzymes are reviewed, with particular focus on their advantages and disadvantages in terms of enzyme loadings, operational stability, undesired adsorption, and costs. Mechanisms and methods for covalent immobilization are also discussed, focusing on the most widespread activating approaches (such as glutaraldehyde, cyanogen bromide, divinylsulfone, carbodiimides, carbonyldiimidazole, sulfonyl chlorides, chlorocarbonates, N-hydroxysuccinimides).
This paper references
10.1016/j.biotechadv.2011.09.003
Immobilization strategies to develop enzymatic biosensors.
A. Sassolas (2012)
10.1016/J.CHROMA.2004.08.010
Development and evaluation of N-hydroxysuccinimide-activated silica for immobilizing human serum albumin in liquid chromatography columns.
H. S. Kim (2004)
10.1016/J.APSUSC.2012.11.120
Preparation of amino-functionalized silica in aqueous conditions
F. Cuoq (2013)
10.1016/S1369-703X(03)00142-6
Stability analysis of Bacillus stearothermophilus L1 lipase immobilized on surface-modified silica gels
Sangpill Hwang (2004)
10.3775/JIE.91.992
Comparison of Softwood and Hardwood Lignocresol-immobilized Cellulases
A. Kobayashi (2012)
10.1002/3527607668
Carrier-bound Immobilized Enzymes: Principles, Application and Design
L. Cao (2005)
Blue enzymes for green chemistry
S Riva (2006)
10.1016/0003-2697(81)90020-8
Procedures for the analysis of cyanogen bromide-activated Sepharose or Sephadex by quantitative determination of cyanate esters and imidocarbonates.
J. Kohn (1981)
10.1039/C2SC01103D
Order-of-magnitude enhancement of an enzymatic hydrogen-air fuel cell based on pyrenyl carbon nanostructures
S. Krishnan (2012)
10.1016/J.MOLCATA.2008.04.001
Degradation of Alizarin Red S under mild experimental conditions by immobilized 5,10,15,20-tetrakis(4-sulfonatophenyl)porphine–Mn(III) as a biomimetic peroxidase-like catalyst
P. Zucca (2008)
10.1021/JA00053A020
A new family of mesoporous molecular sieves prepared with liquid crystal templates
J. S. Beck (1992)
10.1021/BP00030A001
New Materials for Biotechnology: Chromatographic Stationary Phases Based on Zirconia
J. Nawrocki (1994)
10.1002/ZAAC.19542760110
Über Siliciumchalkogenide. VI. Zur Kenntnis der faserigen Siliciumdioxyd‐Modifikation
A. Weiss (1954)
10.1016/J.CATTOD.2007.03.023
Covalent immobilization of trypsin on to siliceous mesostructured cellular foams to obtain effective biocatalysts
A. Jarzȩbski (2007)
10.1023/A:1010091625981
Synthesis of Magnetite Nanoparticles by Precipitation with Forced Mixing
Yihua Zhu (1999)
10.1007/978-1-60761-895-9_5
Nanoporous silica glass for the immobilization of interactive enzyme systems.
A. Buthe (2011)
10.5860/choice.37-0940
Advanced Inorganic Chemistry
F. Cotton (1999)
10.1016/J.MOLCATB.2007.12.006
Comparison among immobilised lipases on macroporous polypropylene toward biodiesel synthesis
A. Salis (2008)
10.1016/J.APSUSC.2014.02.071
The effects of the chemical composition of titanate nanotubes and solvent type on 3-aminopropyltriethoxysilane grafting efficiency
Patricia I. Pontón (2014)
Enzymes immobilized to cellulose.
M. Lilly (1976)
10.1016/0076-6879(87)35066-9
[5] Immobilization of proteins and ligands using chlorocarbonates
T. Miron (1987)
10.1016/s0167-2991(06)x8508-7
Characterization and Chemical Modification of the Silica Surface
E. Vansant (1995)
Montmorillonite; Mineralogical Society of America: Chantilly
J. W. Anthony (2011)
10.1016/0021-9517(63)90004-6
Catalytic Aluminas I. Surface Chemistry of Eta and Gamma Alumina
D. S. Maciver (1963)
10.1126/science.269.5228.1242
Templating of Mesoporous Molecular Sieves by Nonionic Polyethylene Oxide Surfactants
S. Bagshaw (1995)
10.1021/JA00746A021
Sulfonate leaving groups, structure and reactivity. 2,2,2-Trifluoroethanesulfonate
R. K. Crossland (1971)
10.1021/CM0485658
Ordered Mesoporous Materials for Bioadsorption and Biocatalysis
M. Hartmann (2005)
10.1073/PNAS.72.3.1055
Structure of a soluble super-active insulin is revealed by the nature of the complex between cyanogen-bromide-activated sepharose and amines.
M. Wilchek (1975)
10.1016/0076-6879(87)35062-1
Characterization of immobilized biocatalysts.
K. Buchholz (1987)
10.1016/J.MOLCATB.2007.09.016
Polyethyleneimine (PEI) functionalized ceramic monoliths as enzyme carriers: Preparation and performance
K. D. Lathouder (2008)
10.1016/J.SNB.2007.01.035
Immobilization of mushroom tyrosinase on controlled pore glass: Effect of chemical modification
A. Girelli (2007)
10.1021/CR0500535
Titanium dioxide nanomaterials: synthesis, properties, modifications, and applications.
X. Chen (2007)
10.1016/j.nano.2013.08.008
Recent development of silica nanoparticles as delivery vectors for cancer imaging and therapy.
Xu Wu (2014)
10.1007/BF02778260
p-Nitrophenylcyanate—An efficient, convenient, and Nonhazardous substitute for cyanogen bromide as an activating agent for sepharose
J. Kohn (1983)
10.1016/j.msec.2014.02.011
Covalent immobilization of porcine pancreatic lipase on carboxyl-activated magnetic nanoparticles: characterization and application for enzymatic inhibition assays.
Yuan-Ting Zhu (2014)
10.1016/J.CERAMINT.2013.11.128
Novel glass ceramic foams materials based on red mud
Yuxi Guo (2014)
Immobilization of enzymes on nylon. In Methods in Enzymology; Klaus, M., Ed.
W. E. Hornby (1976)
10.1007/s11051-012-0763-2
Preparation of superparamagnetic sodium alginate nanoparticles for covalent immobilization of Candida rugosa lipase
X. Liu (2012)
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license
Affinity immobilization. Methods Enzymol
B Mattiasson (1988)
10.1007/s10562-013-1151-y
Production of Biodiesel Using a Nanoscaled Immobilized Lipase as the Catalyst
Yulong Liu (2013)
Chemistry of Zeolites and Related Porous Materials: Synthesis and Structure
R. Xu (2007)
10.1002/anie.200705238
Proteins in mesoporous silicates.
Sarah Hudson (2008)
10.1016/0014-5793(83)80905-3
1‐Cyano‐4‐dimethylamino pyridinium tetrafluoroborate as a cyanylating agent for the covalent attachment of ligand to polysaccharide resins
J. Kohn (1983)
10.1016/J.CHEMPHYS.2013.06.024
Structural models of activated γ-alumina surfaces revisited: Thermodynamics, NMR and IR spectroscopies from ab initio calculations ☆
A. R. Ferreira (2013)
10.1007/BF00265455
Radiation grafted polyethylene as carrier for protein immobilization
D. Müller-Schulte (1982)
10.1016/0141-0229(82)90109-0
Mechanism of activation of Sepharose and Sephadex by cyanogen bromide
J. Kohn (1982)
10.1016/J.PROCBIO.2012.04.019
Versatility of glutaraldehyde to immobilize lipases: Effect of the immobilization protocol on the properties of lipase B from Candida antarctica
Oveimar Barbosa (2012)
10.1038/359710A0
Ordered mesoporous molecular sieves synthesized by a liquid-crystal template mechanism
C. Kresge (1992)
10.1021/bm400762h
Heterofunctional supports in enzyme immobilization: from traditional immobilization protocols to opportunities in tuning enzyme properties.
Oveimar Barbosa (2013)
10.1016/0003-2697(86)90176-4
Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions.
J. V. Staros (1986)
10.1016/j.saa.2014.01.004
Monodispersed silica nanoparticles as carrier for co-immobilization of bi-enzyme and its application for glucose biosensing.
H. Yang (2014)
10.1016/0304-5102(94)00108-1
Immobilization of lipase from Candida cylindracea on inorganic supports
JoséM. Moreno (1994)
10.1016/J.ENZMICTEC.2007.01.018
Improvement of enzyme activity, stability and selectivity via immobilization techniques
C. Mateo (2007)
10.30638/EEMJ.2007.006
Irreversible affinity immobilization of lentil seedling amine oxidase with activity retention
E. Sanjust (2007)
10.1007/s11244-012-9905-0
Recent Progress in Biocatalysis with Enzymes Immobilized on Mesoporous Hosts
Z. Zhou (2012)
10.1590/S0104-66321999000200005
Influence of activation on the multipoint immobilization of penicillin G acylase on macroporous silica
H. C. T. Cardias (1999)
Covalent immobilization of proteins by techniques which permit subsequent release.
H. Horton (1987)
10.1016/S0076-6879(76)44012-0
[10] Covalent coupling methods for inorganic support materials
H. Weetall (1976)
10.1039/C39930000680
Synthesis of highly ordered mesoporous materials from a layered polysilicate
S. Inagaki (1993)
Adsorption on Silica Surfaces; Papirer
V. Y. Davydov (2000)
10.1126/SCIENCE.279.5350.548
Triblock copolymer syntheses of mesoporous silica with periodic 50 to 300 angstrom pores
Y Zhao (1998)
10.1016/j.colsurfb.2010.11.001
N,N'-Carbonyldiimidazole-mediated functionalization of superparamagnetic nanoparticles as vaccine carrier.
J. Ho (2011)
10.1016/0076-6879(87)35068-2
1,1'-Carbonyldiimidazole-mediated immobilization of enzymes and affinity ligands.
M. Hearn (1987)
10.1016/J.CEJ.2007.07.030
Modification of magnetite nanoparticles via surface-initiated atom transfer radical polymerization (ATRP)
Y. Zhou (2008)
10.1039/C0NJ00161A
Hemoglobin immobilized on mesoporous silica as effective material for the removal of polycyclic aromatic hydrocarbons pollutants from water
Paco Laveille (2010)
10.1016/J.SUPFLU.2013.09.010
Gradual hydrophobic surface functionalization of dry silica aerogels by reaction with silane precursors dissolved in supercritical carbon dioxide
L. M. Sanz-Moral (2013)
10.1023/A:1015287910502
Polyhedral Oligomeric Silsesquioxane (POSS) Polymers and Copolymers: A Review
Gui-zhi Li (2001)
10.1016/J.APCATB.2013.02.032
The role of surface defect sites of titania nanoparticles in the photocatalysis: Aging and modification
M. Radoičić (2013)
10.1180/dhz
An Introduction to the Rock-Forming Minerals
W. A. Deer (1966)
10.1016/S0076-6879(76)44007-7
[5] Immobilization of enzymes to various acrylic copolymer
R. Mosbach (1976)
Enzymes immobilized to cellulose. In Methods in Enzymology; Klaus, M., Ed.
M. D. Lilly (1976)
Adsorption and inorganic bridge formations.
R. Messing (1976)
10.1016/j.aca.2008.05.070
Role of carbon nanotubes in electroanalytical chemistry: a review.
L. Agüí (2008)
Conformational changes and catalytic competency of hydrolases adsorbing on fumed silica nanoparticles: I
J. C. Cruz (2010)
Immobilization of enzymes to agar, agarose, and sephadex support. In Methods in Enzymology; Klaus, M., Ed.
J. Porath (1976)
10.1016/0006-291X(76)91101-3
Immobilization of enzymes by covalent binding to amine supports via cyanogen bromide activation.
J. Schnapp (1976)
10.1016/J.SEPPUR.2012.01.031
Horseradish peroxidase immobilized on the silane-modified ceramics for the catalytic oxidation of simulated oily water
Weichen Wang (2012)
10.1016/J.MOLCATB.2013.10.019
Current status and trends in enzymatic nanoimmobilization
E. P. Cipolatti (2014)
10.1021/BC9600224
Preparation of characterization of poly(ethylene glycol) vinyl sulfone.
M. Morpurgo (1996)
10.1016/S1369-7021(06)71388-8
Immobilizing catalysts on porous materials
X. Zhao (2006)
Affinity immobilization.
B. Mattiasson (1988)
10.1021/CR3003054
Silica surface features and their role in the adsorption of biomolecules: computational modeling and experiments.
A. Rimola (2013)
10.1021/la8024827
Attachment of 3-(Aminopropyl)triethoxysilane on silicon oxide surfaces: dependence on solution temperature.
Robert M. Pasternack (2008)
Immobilization of proteins and ligands using chlorocarbonates.
T. Miron (1987)
10.1016/S0076-6879(74)34007-4
Porous glass for affinity chromatography applications.
H. Weetall (1974)
10.1016/J.MICROMESO.2006.08.039
Carbon–ceramic composites for enzyme immobilization
K. D. Lathouder (2007)
10.1016/J.MOLCATB.2014.01.025
Immobilization of endo-inulinase on non-porous amino functionalized silica nanoparticles
Mahsan Karimi (2014)
10.1016/S1010-6030(03)00077-7
Synthesis of photocatalytic TiO2 nanoparticles: optimization of the preparation conditions
Y. Bessekhouad (2003)
10.1006/JCAT.1995.1118
Titania-Silica Mixed Oxides: I. Influence of Sol-Gel and Drying Conditions on Structural Properties
D. Dutoit (1995)
10.1039/B907869J
Biocatalysis with enzymes immobilized on mesoporous hosts: the status quo and future trends
M. Hartmann (2010)
10.1021/JA974025I
Nonionic Triblock and Star Diblock Copolymer and Oligomeric Surfactant Syntheses of Highly Ordered, Hydrothermally Stable, Mesoporous Silica Structures
D. Zhao (1998)
10.1016/J.MOLCATB.2013.03.018
Catechol modification and covalent immobilization of catalase on titania submicrospheres
H. Wu (2013)
Biosensors : a practical approach
J. Cooper (2004)
Characterization of immobilized biocatalysts. In Methods in Enzymology; Klaus, M., Ed.
K. Buchholz (1987)
Analitical Uses of Immobilized Biological Compounds for Detection, Medical and Industrial Uses; Guilbault
H. H. Weetall (1988)
10.1007/S12257-009-0188-7
Post-immobilization of modified macromolecular reagents using assembled penicillin acylase for microenvironmental regulation of nanopores and enhancement of enzyme stability
C. Zhou (2010)
Covalent coupling methods for inorganic support materials.
H. Weetall (1976)
10.1039/b920576d
Vinyl sulfone: a versatile function for simple bioconjugation and immobilization.
Julia Morales-Sanfrutos (2010)
10.1016/j.jcis.2009.11.014
Improved enzymatic activity of Thermomyces lanuginosus lipase immobilized in a hydrophobic particulate mesoporous carrier.
M. Sörensen (2010)
10.1006/JSSC.1996.0145
A general survey of quartz and quartz-like materials : Packing distortions, temperature, and pressure effects
E. Philippot (1996)
10.1016/J.CEJ.2012.01.091
Performance of invertase immobilized on glass–ceramic supports in batch bioreactor
A. Albertini (2012)
Covalent coupling of concanavalin A to commercial alumina
M. B. Fadda (1992)
10.1142/9789814280525_0004
MESOPOROUS SILICA NANOPARTICLES: SYNTHESIS AND APPLICATIONS
Juan L Vivero-Escoto (2009)
10.1002/ANIE.200503075
Silica-based mesoporous organic-inorganic hybrid materials.
Frank Hoffmann (2006)
10.1016/0021-9797(80)90449-X
Chemistry of silica
E. Matijević (1980)
10.1016/J.CPLETT.2009.07.063
Thermochemistry for silicic acid formation reaction: Prediction of new reaction pathway
B. Mondal (2009)
10.1128/AEM.67.5.2208-2212.2001
New Pulp Biobleaching System Involving Manganese Peroxidase Immobilized in a Silica Support with Controlled Pore Sizes
T. Sasaki (2001)
10.1006/ABIO.1994.1144
A method for the high efficiency of water-soluble carbodiimide-mediated amidation.
D. Sehgal (1994)
Porous Silica Particles Containing a Crystallized Phase and Method
R. Beau (1970)
10.1016/S0378-4347(00)80845-2
Sulfonyl chloride activation of hydroxylic materials.
W. Scouten (1986)
Compound of silica-vinylsulphone, synthesis and uses of the same
F Santoyo-Gonzalez (2009)
10.1042/BST0351593
Advances in the design of new epoxy supports for enzyme immobilization-stabilization.
C. Mateo (2007)
10.1039/CS9962500061
Surface chemistry of titania (anatase) and titania-supported catalysts
K. Hadjiivanov (1996)
10.1016/0076-6879(87)35070-0
Covalent immobilization of proteins by techniques which permit subsequent release.
Horton Hr (1987)
10.1007/BF02916421
Preparation of immobilized proteins covalently coupled through silane coupling agents to inorganic supports
H. Weetall (1993)
10.1021/JA983218I
MESOCELLULAR SILICEOUS FOAMS WITH UNIFORMLY SIZED CELLS AND WINDOWS
Patrick Schmidt-Winkel (1999)
10.1016/0022-3093(77)90093-X
Phase separation in glass
W. Vogel (1977)
10.1186/1752-153X-6-161
Degradation of textile dyes using immobilized lignin peroxidase-like metalloporphines under mild experimental conditions
P. Zucca (2012)
10.1016/J.MOLCATB.2013.10.016
Improvement of P. aeruginosa 42A2 lipase preparations for FAMEs production, both in immobilized and soluble form
Silvia Cesarini (2014)
10.1002/ADSC.200700082
Enzyme Immobilization: The Quest for Optimum Performance
R. Sheldon (2007)
10.1007/s00216-008-2250-2
Enzyme-functionalized mesoporous silica for bioanalytical applications
Cristina Ispas (2009)
10.1016/J.JBIOTEC.2004.09.015
Improved stabilization of chemically aminated enzymes via multipoint covalent attachment on glyoxyl supports.
F. López-Gallego (2005)
10.1021/JA036080Z
Novel route to periodic mesoporous aminosilicas, PMAs: ammonolysis of periodic mesoporous organosilicas.
T. Asefa (2003)
A survey of enzyme coupling techniques.
W. Scouten (1987)
10.1021/JP212056S
Molecular Structure of 3-Aminopropyltriethoxysilane Layers Formed on Silanol-Terminated Silicon Surfaces
R. Acres (2012)
10.1016/J.MOLCATA.2007.09.017
5,10,15,20-tetrakis(4-sulfonato-phenyl)porphine-Mn(III) immobilized on imidazole-activated silica as a novel lignin-peroxidase-like biomimetic catalyst
P. Zucca (2007)
10.1016/0076-6879(88)37062-X
[60] Affinity immobilization
B. Mattiasson (1988)
10.1201/9780203912225
Handbook of Adhesive Technology, Revised and Expanded
A. Pizzi (2003)
10.1016/J.ENZMICTEC.2009.08.009
Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation
R. Fernández-Lafuente (2009)
10.1007/s12010-010-9110-1
Affinity Covalent Immobilization of Glucoamylase onto ρ-Benzoquinone Activated Alginate Beads: I. Beads Preparation and Characterization
M. S. Mohy Eldin (2011)
10.1016/0021-9673(86)80090-5
Optimization of protein immobilization on 1,1′-carbonyldiimidazole-activated diol-bonded silica
Samuel C. Crowley (1986)
10.1016/J.BEJ.2013.02.003
Convenient one-step purification and immobilization of lipase using a genetically encoded aldehyde tag
A. Wang (2013)
10.3390/ijms11020762
Covalent Anchoring of Chloroperoxidase and Glucose Oxidase on the Mesoporous Molecular Sieve SBA-15
Dirk Jung (2010)
10.1007/s00216-010-3708-6
Protein immobilization at gold–thiol surfaces and potential for biosensing
Marco Frasconi (2010)
10.1016/J.MICROMESO.2007.08.009
Study on the activity and stability of urease immobilized onto nanoporous alumina membranes
Zhengpeng Yang (2008)
10.1016/S0076-6879(76)44005-3
[3] Immobilization of enzymes to agar, agarose, and sephadex support
J. Porath (1976)
10.1016/S0076-6879(76)44009-0
Enzymes covalently bound to polyacrylic and polymethacrylic copolymers.
R. Epton (1976)
10.1016/J.CEJ.2014.04.052
Nanostructured tin dioxide – a promising multipurpose support material for catalytic and biocatalytic applications
M. Dimitrov (2014)
10.1126/science.132.3421.220
First Natural Occurrence of Coesite
E. Chao (1960)
Infrared Spectroscopy Of Adsorbed Species On The Surface Of Transition Metal Oxides
Nadine Eberhardt (2016)
10.1016/J.PROCBIO.2010.07.001
Heterofunctional supports for the one-step purification, immobilization and stabilization of large multimeric enzymes: Amino-glyoxyl versus amino-epoxy supports
J. M. Bolivar (2010)
10.1039/c2cs35231a
Modifying enzyme activity and selectivity by immobilization.
R. Rodrigues (2013)
10.1006/JCIS.1997.4985
Aqueous Suspensions of Fumed Silica and Adsorption of Proteins
Gun'ko (1997)
10.1016/0003-2697(89)90080-8
Alumina-phosphate complexes for immobilization of biomolecules.
M. Coletti-previero (1989)
10.1007/S10971-009-1958-6
Synthesis of silica nanoparticles by modified sol–gel process: the effect of mixing modes of the reactants and drying techniques
Mohammad Jafarzadeh (2009)
10.1016/J.MOLCATB.2011.07.011
α-Amylase immobilization on the silica nanoparticles for cleaning performance towards starch soils in laundry detergents
M. Soleimani (2012)
10.1186/1752-153X-6-23
Preparation and magnetic properties of nano size nickel ferrite particles using hydrothermal method
K. Nejati (2012)
10.1126/science.261.5126.1299
Cooperative Formation of Inorganic-Organic Interfaces in the Synthesis of Silicate Mesostructures
A. Monnier (1993)
10.1016/0003-2697(70)90146-6
Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides.
E. Kaiser (1970)
10.1016/J.ACA.2004.09.009
Cholinesterase immobilisation on the surface of screen-printed electrodes based on concanavalin A affinity
Bogdan Bucur (2005)
10.1016/0141-0229(80)90002-2
Magnetic supports for immobilized enzymes and bioaffinity adsorbents
P. Halling (1980)
10.1007/BF03353657
Development of an Amperometric Hydrogen Peroxide Biosensor based on the Immobilization of Horseradish Peroxidase onto Nickel Ferrite Nanoparticle-Chitosan Composite
Furkan Yalçıner (2011)
10.1016/J.ELECTACTA.2010.12.098
Immobilization of glucose oxidase and platinum on mesoporous silica nanoparticles for the fabrication of glucose biosensor
He Li (2011)
10.1180/MINMAG.1971.038.295.11
Conditions for the Formation of Bayerite and Gibbsite
W. Mchardy (1971)
Adsorption and inorganic bridge formations. In Methods in Enzymology; Klaus, M., Ed.
R. A. Messing (1976)
10.1016/S0167-2991(08)63059-1
Guidelines for the Characterization of Porous Solids
J. Rouquerol (1994)
10.1016/J.APCATA.2009.11.009
Biodegradation of a simulated textile effluent by immobilised-coated laccase in laboratory-scale reactors
J. Osma (2010)
10.1007/BF00156610
PEG activation and ligand binding for the affinity partitioning of proteins in aqueous two-phase systems
B. Andrews (1990)
10.1021/bp010171n
Epoxy Sepabeads: A Novel Epoxy Support for Stabilization of Industrial Enzymes via Very Intense Multipoint Covalent Attachment
C. Mateo (2002)
10.1016/S0076-6879(76)44011-9
[9] Immobilization of enzymes on nylon
W. Hornby (1976)
10.1186/1752-153X-6-1
Development of a novel 96-microwell assay with high throughput for determination of olmesartan medoxomil in its tablets
I. Darwish (2012)
10.1002/JBM.A.10143
Direct attachment of fibronectin to tresyl chloride-activated titanium.
T. Hayakawa (2003)
10.1016/J.TIBTECH.2006.03.006
Laccases: blue enzymes for green chemistry.
S. Riva (2006)
10.1016/S0021-9673(98)00999-6
Purification of monoclonal antibodies from cell culture supernatants using a modified zirconia based cation-exchange support.
A. Clausen (1999)
Tresyl chloride-activated supports for enzyme immobilization.
K. Nilsson (1987)
10.1039/CS9952400073
Puzzles and paradoxes in protein adsorption
J. Ramsden (1995)
10.1021/JA00545A056
Silicon-29 NMR study of the surface of silica gel by cross polarization and magic-angle spinning
G. Maciel (1980)
Energies of strained silica
D. R. Hamann (1997)
10.1016/j.enzmictec.2013.02.018
Catalytic activity and thermostability of enzymes immobilized on silanized surface: influence of the crosslinking agent.
Nesrine Aissaoui (2013)
10.1016/S0956-5663(97)00053-5
Capacitive monitoring of protein immobilization and antigen-antibody reactions on monomolecular alkylthiol films on gold electrodes.
V. Mirsky (1997)
10.1016/B978-0-12-385469-8.00059-9
Porous Ceramic Materials
T. Ohji (2013)
10.1021/AR600032U
Synthesis and functionalization of a mesoporous silica nanoparticle based on the sol-gel process and applications in controlled release.
B. Trewyn (2007)
10.1016/0006-291X(82)90604-0
A new approach (cyano-transfer) for cyanogen bromide activation of Sepharose at neutral pH, which yields activated resins, free of interfering nitrogen derivatives.
J. Kohn (1982)
10.1016/j.actbio.2013.03.036
Functionalized ceramics for biomedical, biotechnological and environmental applications.
L. Treccani (2013)
10.1016/J.MOLCATB.2010.05.010
Chemical modification and immobilisation of lipase B from Candida antarctica onto mesoporous silicates
Jessica Forde (2010)
10.1021/CS200124A
Perspective of Recent Progress in Immobilization of Enzymes
Daniel N. Tran (2011)
10.1016/J.PROCBIO.2010.10.016
Adsorption onto alumina and stabilization of cysteine proteinases from crude extract of Solanum granuloso-leprosum fruits
D. Valles (2011)
10.1385/0-89603-386-4:21
Immobilization of Proteins on Thionyl Chloride-Activated Controlled-Pore Glass
V. G. Janolino (1997)
10.1246/BCSJ.63.988
The preparation of alkyltrimethylammonium-kanemite complexes and their conversion to microporous materials.
T. Yanagisawa (1990)
Chapter 11.2.2—Porous ceramic materials
T. Ohji (2013)
10.1016/J.PROCBIO.2004.12.013
Preparation and characterization of trypsin immobilized on silica gel supported macroporous chitosan bead
F. Xi (2005)
Nadh oxidase activity of the fad-alumina derivative
E. Sanjust (1991)
10.1016/J.PROCBIO.2014.01.028
Tuning of Lecitase features via solid-phase chemical modification: Effect of the immobilization protocol
Cristina Garcia-Galan (2014)
10.1016/0076-6879(87)35065-7
Colored sulfonyl chloride as an activating agent for hydroxylic matrices.
W. Scouten (1987)
Silanes and other Coupling Agents
K. L. Mittal (2007)
10.1134/S0003683812030076
Catalytic properties of a nitrile hydratase immobilized on activated chitosan
Yu. G. Maksimova (2012)
10.1039/c3cs60021a
Immobilization of enzymes on porous silicas--benefits and challenges.
M. Hartmann (2013)
THE SOL-GEL PROCESS
G. Wall (1965)
10.1103/PHYSREVB.55.14784
Energies of strained silica rings
D. Hamann (1997)
10.1016/S0141-0229(98)00084-2
Comparison of techniques for enzyme immobilization on silicon supports
A. Subramanian (1999)
10.1016/j.bios.2012.04.030
Biofuel cell as a power source for electronic contact lenses.
M. Falk (2012)
10.1016/J.COLSURFA.2004.11.033
Applying the Taguchi method to the optimization for the synthesis of TiO2 nanoparticles by hydrolysis of TEOT in micelles
K. Kim (2005)
10.1016/J.MOLCATB.2014.01.017
Novel hybrid materials on the basis of nanostructured tin dioxide and a lipase from Rhizopus delemar with improved enantioselectivity
M. Guncheva (2014)
10.1021/LA015749H
Self-Assembly of Carboxyalkylphosphonic Acids on Metal Oxide Powders
S. Pawsey (2002)
10.1002/cctc.200900288
Physical and Chemical Lipase Adsorption on SBA‐15: Effect of Different Interactions on Enzyme Loading and Catalytic Performance
A. Salis (2010)
10.1016/J.CLAY.2013.11.020
Preparation and characterization of montmorillonite modified with 3-aminopropyltriethoxysilane
P. T. Bertuoli (2014)
10.1021/la904027p
Investigations of chemical modifications of amino-terminated organic films on silicon substrates and controlled protein immobilization.
Joonyeong Kim (2010)
10.1016/J.ENZMICTEC.2005.03.020
Some special features of glyoxyl supports to immobilize proteins
C. Mateo (2005)
10.1021/am301637q
Surface modification of siliceous materials using maleimidation and various functional polymers synthesized by reversible addition-fragmentation chain transfer polymerization.
Hirokazu Seto (2012)
10.1016/J.CERAMINT.2014.02.089
Macroporous ceramics: Novel route using partial sintering of alumina-powder agglomerates obtained by spray-drying
G. Jean (2014)
10.1016/J.CARBPOL.2011.11.007
Synthesis and characterization of chitosan-homocysteine thiolactone as a mucoadhesive polymer
Kotchakorn Juntapram (2012)
Immobilization of enzymes to agar, agarose, and Sephadex supports.
J. Porath (1976)
10.1016/j.colsurfb.2010.06.005
Conformational changes and catalytic competency of hydrolases adsorbing on fumed silica nanoparticles: II. Secondary structure.
J. C. Cruz (2010)
10.1016/j.actbio.2009.06.025
Alkaline phosphatase grafting on bioactive glasses and glass ceramics.
E. Verné (2010)
10.1021/bc800051c
Covalent immobilization of proteins on carbon nanotubes using the cross-linker 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide--a critical assessment.
Yuan Gao (2008)
10.1039/C3RA43666G
CelB and β-glucosidase immobilization for carboxymethyl cellulose hydrolysis
Clara T Tran (2013)
10.1007/978-94-009-2895-4
Analytical uses of immobilized biological compounds for detection, medical, and industrial uses
Industrial Uses (1988)
10.1021/j100566a009
Infrared studies of reactions on oxide surfaces. 7. Mechanism of the adsorption of water and ammonia on dehydroxylated silica
B. Morrow (1976)
10.1016/J.MOLCATB.2008.12.004
Characterization and properties of catalase immobilized onto controlled pore glass and its application in batch and plug-flow type reactors
Özlem Alptekin (2009)
High-pressure crystal chemistry of stishovite
N. Ross (1990)
10.1039/C3RA45991H
Glutaraldehyde in bio-catalysts design: a useful crosslinker and a versatile tool in enzyme immobilization
Oveimar Barbosa (2014)
10.1016/j.colsurfb.2010.03.036
Conformational changes and catalytic competency of hydrolases adsorbing on fumed silica nanoparticles: I. tertiary structure.
Juan C Cruz (2010)
10.1016/J.ENZMICTEC.2008.12.007
Activation of nylon net and its application to a biosensor for determination of glucose in human serum
C. Nan (2009)
10.1016/J.IJGGC.2012.12.010
Catalytic behavior of carbonic anhydrase enzyme immobilized onto nonporous silica nanoparticles for enhancing CO2 absorption into a carbonate solution
Shihan Zhang (2013)
10.1021/JA01465A001
Hydrogen Bonding Studies. V. The Relative Basicities of Ethers, Alkoxysilanes and Siloxanes and the Nature of the Silicon-Oxygen Bond1,2
R. West (1961)
10.1016/j.biotechadv.2011.09.005
Potential applications of enzymes immobilized on/in nano materials: A review.
S. Ansari (2012)
Phase Separation in Glass; North-Holland
O. V. Mazurin (1985)
10.1016/j.msec.2012.12.048
Enhanced stability of catalase covalently immobilized on functionalized titania submicrospheres.
H. Wu (2013)
10.1016/S0076-6879(76)44004-1
Functional groups on enzymes suitable for binding to matrices.
P. Srere (1976)
10.1016/0076-6879(95)51107-5
Thiol/disulfide exchange equilibria and disulfide bond stability.
H. Gilbert (1995)
Immobilization of β-galactosidase on chitosan by 2,4,6-trichloro-1,3,5-triazine cethod. Available online
S Sun (2014)
10.1016/S0076-6879(76)44006-5
[4] Enzymes immobilized to cellulose
M. Lilly (1976)
10.1016/S0076-6879(76)44013-2
[11] Adsorption and inorganic bridge formations
R. Messing (1976)
10.1016/S0076-6879(74)34006-2
Covalent linkage of functional groups, ligands, and proteins to polyacrylamide beads.
J. Inman (1974)
10.1002/ANIE.200702986
The structure of silicate anions in aqueous alkaline solutions.
C. T. Knight (2007)
10.1016/S0927-7757(00)00556-2
The surface chemistry of amorphous silica. Zhuravlev model
L. T. Zhuravlev (2000)
10.1016/S1381-1177(02)00223-0
Influence of different silica derivatives in the immobilization and stabilization of a Bacillus licheniformis protease (Subtilisin Carlsberg)
Lino Ferreira (2003)
10.1002/ELAN.200403343
Amperometric Flow‐Injection Determination of Phenolic Compounds Using a Biosensor with Immobilized Laccase, Peroxidase and Tyrosinase
R. Solná (2005)
Immobilization of enzymes on nylon.
W. Hornby (1976)
10.1016/j.colsurfb.2012.04.042
Immobilization of lipase from Mucor miehei and Rhizopus oryzae into mesoporous silica--the effect of varied particle size and morphology.
Hanna Gustafsson (2012)
10.2144/04375RV01
Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking.
Isabelle Migneault (2004)
10.1016/J.MOLCATA.2013.05.023
Silica nanoparticles immobilized benzoylthiourea ferrous complex as an efficient and reusable catalyst for one-pot synthesis of benzopyranopyrimidines
Sara Amirnejat (2013)
10.1016/J.MOLCATA.2010.01.015
Is the bleaching of phenosafranine by hydrogen peroxide oxidation catalyzed by silica-supported 5,10,15,20-tetrakis-(sulfonatophenyl)porphine-Mn(III) really biomimetic?
P. Zucca (2010)
10.1016/j.jmmm.2006.06.003
Synthesis and magnetic properties of cobalt ferrite (CoFe2O4) nanoparticles prepared by wet chemical route
K.Maaz (2007)
10.1016/J.CARBON.2014.03.022
Immobilization of gamma globulins and polyclonal antibodies of class IgG onto carbon-encapsulated iron nanoparticles functionalized with various surface linkers
M. Popławska (2014)
Immobilization of β-galactosidase on chitosan by 2,4,6-trichloro-1,3,5-triazine cethod
S. Sun (2014)
10.1246/BCSJ.63.988
The Preparation of Alkyltriinethylaininonium–Kaneinite Complexes and Their Conversion to Microporous Materials
YanagisawaTsuneo (2006)
10.1016/J.MICROMESO.2007.12.009
Oxidation of indole using chloroperoxidase and glucose oxidase immobilized on SBA-15 as tandem biocatalyst
Dirk Jung (2008)
10.1007/s10450-014-9623-y
Enzyme immobilization by adsorption: a review
T. Jesionowski (2014)
10.1039/c2cs35450k
Immobilisation of enzymes on mesoporous silicate materials.
E. Magner (2013)
10.1002/(SICI)1097-4628(19971121)66:8<1433::AID-APP2>3.0.CO;2-F
Novel Diazonium-Functionalized Support for Immobilization Experiments
N. Curreli (1997)
10.1016/0003-2697(83)90264-6
High-performance liquid affinity chromatography on silica-bound alcohol dehydrogenase.
K. Nilsson (1983)
10.1021/JA031725G
Toward single-site, immobilized molecular catalysts: site-isolated Ti ethylene polymerization catalysts supported on porous silica.
Michael W. McKittrick (2004)
A comparative study on immobilization of urease on different matrices
C. Selvamurugan (2007)
10.1016/J.MOLCATB.2011.10.002
Immobilized redox enzymatic catalysts: Baeyer–Villiger monooxygenases supported on polyphosphazenes
A. Cuetos (2012)
10.1201/9781482269703
Adsorption on Silica Surfaces
E. Papirer (2000)
10.1016/0076-6879(87)35064-5
[3] Tresyl chloride-activated supports for enzyme immobilization
K. Nilsson (1987)
10.1016/0076-6879(87)35063-3
[2] A survey of enzyme coupling techniques
W. Scouten (1987)
10.1016/0160-9327(80)90074-5
The chemistry of silica: By Ralph K. Iller. Pp. vii+ 866. Wiley, Chichester. 1979, £39.50
L. Glasser (1980)
10.1021/bc1003372
A versatile method for functionalizing surfaces with bioactive glycans.
F. Cheng (2011)
10.1039/C2JM14239B
Functional self-assembled monolayers (SAMs) of organic compounds on gold nanoparticles
S. A. Jadhav (2012)
10.1016/S0921-0423(98)80086-5
Covalent immobilization of crude and partially-purified lipases onto inorganic supports: stability and hyperactivation.
A. Alcántara (1998)
10.1016/J.PROCBIO.2009.04.015
Immobilization–stabilization of the lipase from Thermomyces lanuginosus: Critical role of chemical amination
Rafael C. Rodrigues (2009)
10.1016/J.CATCOM.2006.07.009
Immobilization of α-amylase on zirconia: a heterogeneous biocatalyst for starch hydrolysis
R. Reshmi (2007)



This paper is referenced by
10.3103/S1068375517060060
Voltammetric responses of black radish enzyme preparation immobilized on kaolin and aerosil
K. D. Pershina (2017)
BIOCONVERSION OF WASTE FEEDSTOCK INTO USEFUL PRODUCTS: AN INSIGHT INTO RECENT STUDIES AND INVESTIGATIONS
Sunil Jayant Kulkarni (2016)
10.1016/j.jhazmat.2019.05.032
Immobilized laccase on polyimide aerogels for removal of carbamazepine.
Carolina Simón-Herrero (2019)
10.1016/j.ijbiomac.2020.11.127
Immobilization of enzymes on nanoinorganic support materials: An update.
Zahra Ashkan (2020)
10.1039/C5RA19571C
Cellulase stabilization by crosslinking with ethylene glycol dimethacrylate and evaluation of its activity including in a water–ionic liquid mixture
Shivani Jamwal (2016)
10.1007/s10930-017-9702-5
Immobilization Increases the Stability and Reusability of Pigeon Pea NADP+ Linked Glucose-6-Phosphate Dehydrogenase
Siddhartha Singh (2017)
10.1515/tjb-2017-0155
Covalent immobilization of an alkaline protease from Bacillus licheniformis
Yakup Aslan (2018)
10.1007/s00253-020-10875-7
Biocatalytic production of Ɛ-caprolactone using Geotrichum candidum cells immobilized on functionalized silica.
André Leonardo Patrício Silva (2020)
10.1108/SR-04-2018-0084
An overview of biomolecules, immobilization methods and support materials of biosensors
Melis Asal (2019)
10.1042/BSR20150154
Acetylcholinesterase immobilization and characterization, and comparison of the activity of the porous silicon-immobilized enzyme with its free counterpart
M. Saleem (2016)
Feruloyl esterases - Evaluation of their potential for biotechnological applications
Cyrielle Bonzom (2019)
10.1002/JCTB.4787
Immobilization of a multi‐enzyme system for L‐amino acids production
María José Rodríguez-Alonso (2016)
10.1515/boca-2016-0001
Nanobiocatalysis: Nanostructured materials – a minireview
Magdalena de Jesús Rostro-Alanis (2016)
10.1007/s12010-020-03348-0
Robust Magnetized Oil Palm Leaves Ash Nanosilica Composite as Lipase Support: Immobilization Protocol and Efficacy Study
E. Onoja (2020)
10.1002/cctc.201500812
Bimetallic Nanoshells as Platforms for Metallo‐ and Biometallo‐Catalytic Applications
C. M. Kisukuri (2016)
10.1007/s10529-020-02828-x
RETRACTED ARTICLE: Immobilization of lactoperoxidase on ZnO nanoparticles with improved stability
Mehrnaz Movahedi (2020)
10.1039/d0ra04287k
Recent advances and future perspectives of sol–gel derived porous bioactive glasses: a review
K. Deshmukh (2020)
10.1080/21691401.2017.1345920
Synthesis of glucose oxidase-PEG aldehyde conjugates and improvement of enzymatic stability
G. Vardar (2018)
10.1134/S0036024420100027
First-Principles Study of Adsorption of XCN (X = F, Cl, and Br) on Surfaces of Polyaniline
M. Arjmandi (2020)
10.1016/j.reactfunctpolym.2020.104676
Improved development in magnetic Xyl-CLEAs technology for biotransformation of agro-industrial by-products through the use of a novel macromolecular cross-linker
J. S. Hero (2020)
10.1002/CEAT.201800657
Adsorption Kinetics of Cellulase and Xylanase Immobilized on Magnetic Mesoporous Silica
Nurul Jannah Sulaiman (2019)
10.1002/ADFM.201703074
Exosome as a Vehicle for Delivery of Membrane Protein Therapeutics, PH20, for Enhanced Tumor Penetration and Antitumor Efficacy
Yeonsun Hong (2018)
10.3390/molecules191220671
Editorial: Special Issue — Enzyme Immobilization
R. Fernández-Lafuente (2014)
10.1016/J.ELECTACTA.2018.10.084
A solid-state electrochemiluminescence biosensor for Con A detection based on CeO2@Ag nanoparticles modified graphene quantum dots as signal probe
Fumei Zuo (2019)
10.1002/tcr.201600007
Chemical Modification in the Design of Immobilized Enzyme Biocatalysts: Drawbacks and Opportunities.
N. Rueda (2016)
10.1080/10826068.2017.1405025
Preparation and characterization of alkaline phosphatase, horseradish peroxidase, and glucose oxidase conjugates with carboxylated carbon nano-onions
Vibol Sok (2018)
10.1080/07388551.2018.1531822
Multi-point enzyme immobilization, surface chemistry, and novel platforms: a paradigm shift in biocatalyst design
M. Bilal (2019)
10.1016/j.foodchem.2017.11.026
Highly stable novel silica/chitosan support for β-galactosidase immobilization for application in dairy technology.
Natália Carminatti Ricardi (2018)
10.1002/CHIN.201521306
Inorganic Materials as Supports for Covalent Enzyme Immobilization: Methods and Mechanisms
P. Zucca (2015)
10.1007/978-981-15-7998-1_11
Immobilization of Enzymes onto Silica-Based Nanomaterials for Bioprocess Applications
Devendra Sillu (2021)
10.1007/s10529-019-02741-y
Immobilization of lactoperoxidase on Fe3O4 magnetic nanoparticles with improved stability
Seyed Ziyae Aldin Samsam Shariat (2019)
10.1016/j.carbpol.2019.115471
Urease immobilization on magnetic micro/nano-cellulose dialdehydes: Urease inhibitory of Biginelli product in Hantzsch reaction by urea.
F. Tamaddon (2020)
See more
Semantic Scholar Logo Some data provided by SemanticScholar