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PD-L1 Promotes Head And Neck Squamous Cell Carcinoma Cell Growth Through MTOR Signaling

A. Zheng, F. Li, F. Chen, J. Zuo, L. Wang, Y. Wang, S. Chen, B. Xiao, Zezhang Tao
Published 2019 · Chemistry, Medicine

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Programmed death-ligand 1 (PD-L1), an immune co-stimulatory molecule, is expressed on various cancer cells and the surface of immune cells. Its overexpression on tumor cells suppresses the immune response to promote tumor cell immune escape. The present study demonstrated that PD-L1 was critical in head and neck squamous cell carcinoma (HNSCC) carcinogenesis. Immunohistochemical analysis of HNSCC tissue microarrays revealed that PD-L1 was overexpressed in tumor tissue, and its expression increased as tumor malignancy progressed (from grade I to IV). Subsequently, the expression of PD-L1 was knocked down or overexpressed in the HNSCC cell lines Cal-27 and Fadu. It was demonstrated that PD-L1 significantly induced HNSCC cell proliferation and colony forming ability. Cell proliferation was also promoted in Cal-27 cell xenograft BALB/c nude mice. In addition, it was determined by western blotting that the PD-L1-mediated increase in HNSCC cell proliferation may have been associated with the activation of mammalian target of rapamycin (mTOR) signaling pathway. Furthermore, mTOR inhibitor (rapamycin) prevented the increase in proliferation. Based on these results, it was concluded that PD-L1 promoted cell proliferation of HNSCC cells through mTOR signaling, and blocking PD-L1 may be conducive in HNSCC therapy.
This paper references
Discrete signaling mechanisms of mTORC1 and mTORC2: Connected yet apart in cellular and molecular aspects.
M. Jhanwar-Uniyal (2017)
TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method
KJ Livak (2001)
B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin 10 secretion
S. Dzik (2000)
Immune checkpoint blockade: a common denominator approach to cancer therapy.
S. Topalian (2015)
Targeting the PD-1/B7-H1(PD-L1) pathway to activate anti-tumor immunity.
Suzanne L. Topalian (2012)
CD80 Expressed by CD8+ T Cells Contributes to PD-L1-Induced Apoptosis of Activated CD8+ T Cells
M. R. Rollins (2017)
Increase of programmed death-1-expressing intratumoral CD8 T cells predicts a poor prognosis for nasopharyngeal carcinoma
Mei-Chi Hsu (2010)
PLC and PI3K/Akt/mTOR signalling in disease and cancer.
M. Follo (2015)
PD - L 1 - specific T cells
SM Ahmad (2016)
Metabolic Competition in the Tumor Microenvironment Is a Driver of Cancer Progression
Chih-Hao Chang (2015)
Expression of programmed death ligand 1 (PD-L1) is associated with poor prognosis in human breast cancer
S. Muenst (2014)
Melanoma Cell-Intrinsic PD-1 Receptor Functions Promote Tumor Growth
S. Kleffel (2015)
Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
H. Nishimura (1999)
B7-H1 blockade augments adoptive T-cell immunotherapy for squamous cell carcinoma.
S. Strome (2003)
Tumor-Intrinsic PD-L1 Signals Regulate Cell Growth, Pathogenesis, and Autophagy in Ovarian Cancer and Melanoma.
Curtis A. Clark (2016)
CD274 promotes cell cycle entry of leukemia-initiating cells through JNK/Cyclin D2 signaling
X. Fang (2016)
Intrinsic and extrinsic control of expression of the immunoregulatory molecule PD-L1 in epithelial cells and squamous cell carcinoma.
Patcharee Ritprajak (2015)
Dietary fiber intake and head and neck cancer risk: A pooled analysis in the International Head and Neck Cancer Epidemiology consortium
D. Kawakita (2017)
Expression of B7‐H1 in breast cancer patients is strongly associated with high proliferative Ki‐67‐expressing tumor cells
H. Ghebeh (2007)
Changes in survival in head and neck cancers in the late 20th and early 21st century: a period analysis.
D. Pulte (2010)
Safety, activity, and immune correlates of anti-PD-1 antibody in cancer.
S. Topalian (2012)
B7-H1 overexpression regulates epithelial-mesenchymal transition and accelerates carcinogenesis in skin.
Yujia Cao (2011)
Alcohol and cigarette consumption predict mortality in patients with head and neck cancer: a pooled analysis within the International Head and Neck Cancer Epidemiology (INHANCE) Consortium
L. Giraldi (2017)
Molecular and Biochemical Aspects of the PD-1 Checkpoint Pathway.
V. Boussiotis (2016)
Tumor immune microenvironment in head and neck cancers
S. M. Chen (2020)
Bidirectional crosstalk between PD-L1 expression and epithelial to mesenchymal transition: Significance in claudin-low breast cancer cells
Abdullah Alsuliman (2015)
Coinhibitory Pathways in Immunotherapy for Cancer.
S. Baumeister (2016)
Signaling pathway and dysregulation of PD1 and its ligands in lymphoid malignancies.
Y. Xia (2016)
A Patient-Derived, Pan-Cancer EMT Signature Identifies Global Molecular Alterations and Immune Target Enrichment Following Epithelial-to-Mesenchymal Transition
M. Mak (2015)
Blockade of B7-H1 and PD-1 by monoclonal antibodies potentiates cancer therapeutic immunity.
Fumiya Hirano (2005)
B7-H1 Expression Is Associated with Poor Prognosis in Colorectal Carcinoma and Regulates the Proliferation and Invasion of HCT116 Colorectal Cancer Cells
S. Shi (2013)
GSK3 and its interactions with the PI3K/AKT/mTOR signalling network.
Miguel A Hermida (2017)
Role of PD-1 in Immunity and Diseases.
Kenji Chamoto (2017)
Predictive correlates of response to the anti-PD-L1 antibody MPDL3280A in cancer patients
R. Herbst (2014)
The role of the PD-L1:PD-1 pathway in squamous cell carcinoma of the head and neck.
D. Zandberg (2014)
Tumor-associated B7-H1 promotes T-cell apoptosis: A potential mechanism of immune evasion
H. Dong (2002)
Inhibition of mTORC1 by Astrin and Stress Granules Prevents Apoptosis in Cancer Cells
K. Thedieck (2013)
Author Correction: Sin1 phosphorylation impairs mTORC2 complex integrity and inhibits downstream Akt signalling to suppress tumorigenesis
P. Liu (2019)
PD-L1-specific T cells
S. M. Ahmad (2015)
Dual inhibition of PI3K/mTOR signaling in chemoresistant AML primary cells.
J. Bertacchini (2018)
Safety and activity of anti-PD-L1 antibody in patients with advanced cancer.
J. Brahmer (2012)
Analysis of relative gene expression data using real - time quantitative PCR and the 2 - ΔΔ C T method
KJ Livak (2001)
Identification and Characterization of MEDI4736, an Antagonistic Anti–PD-L1 Monoclonal Antibody
R. Stewart (2015)
PD-L1 regulates the development, maintenance, and function of induced regulatory T cells
Loise M. Francisco (2009)
Metastasis is regulated via microRNA-200/ZEB1 axis control of tumor cell PD-L1 expression and intratumoral immunosuppression
Limo Chen (2014)
Biomarkers predict enhanced clinical outcomes with afatinib versus methotrexate in patients with second-line recurrent and/or metastatic head and neck cancer
E. Cohen (2017)

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