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One Bis-Boc-L -alanine-1,8-naphthyridine ligand containing amino acid by amido bond named 2,7-Bis-Boc-L -alanine-1,8-naphthyridine (L1) was synthesized and characterized. And there absorption and fluorescence spectra in methanol is presented. Then, the comprehensive study of spectroscopic properties upon titration of L1 with heavy metal ions as ion source in CH3 OH was investigated. It shows compound L1 can use as a fluorescent probe for heavy metal ions like Cr3 + .