Benzodiazepines: Sample Preparation And HPLC Methods For Their Determination In Biological Samples.
V. Samanidou, M. N. Uddin, I. Papadoyannis
Published 2009 · Medicine, Chemistry
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Benzodiazepines (BDZs) belong to a group of substances known for their sedative, antidepressive, muscle relaxant, tranquilizer, hypnotic and anticonvulsant properties. Their determination in biological fluids is essential in clinical assays as well as in forensics and toxicological studies. Researchers focus on the development of rapid, accurate, precise and sensitive methods for the determination of BDZs and their metabolites. A large number of analytical methods using different techniques have been reported, but none can be considered as the method of choice. BDZs are usually present at trace levels (microgram or nanogram per milliliter) in a complex biological matrix and the potentially interfering compounds must be isolated by various extraction techniques before analysis. An extended and comprehensive review is presented herein, focusing on sample preparation (pretreatment and extraction) and HPLC conditions applied by different authors. These methods enable bioanalysts to achieve detection limits down to 1-2 ng/ml using UV/diode array detection, readily available in most laboratories, and better than 1 ng/ml using electron capture detection, which is lower than that obtained using a nitrogen phosphorus detector. MS interfaced with electrospray ionization offered a similar sensitivity, while negative chemical ionization MS or sonic spray ionization MS provided sensitivity down to 0.1 ng/ml.
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HPLC-DAD Determination of CNS-Acting Drugs in Human Blood, Plasma, and Serum
Ana Moreno (2014)
Electrochemical detection of benzodiazepines, following liquid chromatography, for applications in pharmaceutical, biomedical and forensic investigations
K. C. Honeychurch (2014)
Mixed-mode SPE for a multi-residue analysis of benzodiazepines in whole blood using rapid GC with negative-ion chemical ionization MS.
Nerijus Karlonas (2013)
An Overview on Total Analytical Methods for the Detection of 1,4-Benzodiazepines
Mohammad Nasir Uddin (2014)
Gas chromatography-tandem mass spectrometry assay for the quantification of four benzodiazepines and citalopram in eleven postmortem rabbit fluids and tissues, with application to animal and human samples.
Nathalie Cartiser (2011)
Novel univariate and multivariate regression methods for the simultaneous analysis of ternary mixture of 1, 4-benzodiazepines in dosage forms and human urine.
Fathallah F Belal (2018)
Simplifying sample preparation using fabric phase sorptive extraction technique for the determination of benzodiazepines in blood serum by high-performance liquid chromatography.
V. Samanidou (2016)
BIOANALYTICAL METHOD DEVELOPMENT AND COMPARATIVE BIOAVAILABILITY STUDY OF CLOBAZAM TABLETS IN ALBINO RATS PLASMA USING RP-HPLC METHOD
P. Venkatesh (2010)