Online citations, reference lists, and bibliographies.
← Back to Search

Mechanisms Of Sexual Polyploidization And Inheritance In Triploid Citrus Populations

J. Ibañez
Published 2013 · Biology

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
Citrus is the main fruit crop in the world and Spain is the 6th producer and the major exporter for the fresh fruit market. Seedlessness is one of the most important fruit quality traits for this market since consumers do not accept seedy fruits. Recovery of triploid hybrids has become an important breeding strategy to develop new seedless citrus varieties and several of them have been already released from citrus breeding programs worldwide. Despite the undisputable importance of polyploidy in plant species, their genetics are much less well known than those of their diploid counterparts. Citrus triploid hybrids can be routinely recovered from sexual polyploidization (2x × 2x) or interploid crosses (2x × 4x and 4x × 2x). In 2x × 2x sexual crosses, spontaneous triploid hybrids arise from the union of an unreduced (2n) megagametophyte with haploid pollen. In the case of interploid sexual crosses (2x × 4x and 4x × 2x), triploid hybrids result from the fecundation of a diploid gamete arising from the tetraploid parent and a haploid gamete arising from the diploid parent. The genetic and phenotypic structures of triploid populations greatly depend on the parental heterozygosity restitution (HR) in the diploid gamete at each locus, which is mainly affected by the triploid recovery strategy. In 2x × 2x crosses, HR depends on the underlying mechanism leading to the unreduced gamete formation, which are genetically equivalent to First Division Restitution (FDR) or Second Division Restitution (SDR) mechanisms. Moreover, under each restitution mechanism, HR also depends on the locus-centromere genetic distance. In the case of interploid crosses, parental heterozygosity restitution from tetraploid parents depends on the double reduction frequency. In citrus, the unreduced gamete formation mechanism is still controversial; FDR has been the mechanism proposed for sweet orange, whereas SDR has been proposed for clementine. On the other hand, inferring the allelic configurations of genetic markers is a main challenge in polyploidy crops to infer genotypic and gametic structures with the objective to analyze meiosis and inheritance mechanisms. According to this scientific context, the objectives of the thesis where: (i) to develop a new approach for allele dosis assignation when using co-dominant markers, (ii) to implement and apply methods for the analysis of 2n gametes origin and locate centromeres, and (iii) to take advantage of this knowledge to locate a major gene of resistance to Alternaria Brown Spot (ABS) which is a major constraint for triploid mandarin breeding. For microsatellite (SSR) markers, we have demonstrated that triploid progeny genotyping can be successfully performed using the microsatellite allele-counting peak ratio (MAC-PR) method. However, SSR analysis remains relatively costly and time consuming compared with actual SNP genotyping methods. Moreover, with the increasing availability of EST databases and whole genome sequences, SNPs have become the most abundant and powerful polymorphic markers that can be selected along the entire genome. In this thesis, a new method based on competitive allele-specific PCR has been developed to assign SNP allele dosage in an accurate, simple, and cost effective way. Combining the MAC-PR and the new developed SNP genotyping methods offers the possibility to utilize a broad range of molecular markers in genotyping triploid genotypes. Both methods have been used in further works included in this thesis. SDR has been demonstrated as the mechanism underlying unreduced gamete production in `Fortune' mandarin by genotyping triploid progenies with SSR markers. In addition, a new method to locate the centromere, based on the best fit between observed heterozygosity restitution within a linkage group and theoretical functions under either partial or no chiasma interference hypotheses has been developed and successfully applied. To expand the knowledge of the mechanism underlying unreduced gamete formation to other citrus genotypes besides clementines and `Fortune? mandarin, a maximum likelihood method based on parental heterozygosity restitution of centromeric loci was developed and successfully applied in sixteen mandarin cultivars. The new method developed in the study allows inferring the restitution mechanism both at population level and even at individual level. Maternal origin of 2n gametes was confirmed for all triploid hybrids and SDR was proposed as the restitution mechanism for all analyzed progenies. The information acquired from the mode of heterozygosity restitution in citrus was useful to determine the genetic and phenotypic structures of new triploid populations arising from different breeding strategies. We studied these structures for the resistance to Alternaria brown spot (ABS), a serious fungal disease producing necrotic lesions on fruits and young leaves in susceptible citrus genotypes. In the present work, different approaches were combined taking advantage of the particular genetic structures of 2n gametes resulting from SDR to map a genome region linked to ABS resistance in triploid citrus progeny. The monolocus dominant inheritance of the susceptibility, proposed on the basis of diploid population studies, was corroborated in triploid progeny. A 3.3 Mb genomic region linked to ABS resistance was located near the centromere on chromosome III, which includes clusters of resistance genes. SSR and SNP markers were developed for an efficient early selection of ABS resistant hybrids and they are currently used in our breeding program to perform marker assisted selection. The knowledge obtained in this thesis on the mechanism of sexual polyploidization and inheritance of concrete traits in citrus will allow implementing much more efficient triploid breeding programs on the basis of current and future needs. Indeed, applied outcomes of this PhD are already routinely used in the IVIA triploid breeding program.
This paper references
A New Mode of Segregation in Gregory's Tetraploid Primulas
H. Muller (1914)
Studies on the mechanisms of parthenogenesis
Å. Gustafsson (1935)
Investigation on polyploidy in Citrus
WK Lapin (1937)
The botany of citrus and its wild relatives of the orange subfamily (family Rutaceae, subfamily Aurantioideae)
W. T. Swingle (1943)
On the big leaf and small leaf strain of trifoliate orange (Poncirus trifoliata)
T. Iwasaki (1943)
Variation and Evolution in Plants
G. Stebbins (1950)
Induction of chromosome doubling at meiosis by the elongate gene in maize.
M. Rhoades (1966)
Some methods for classification and analysis of multivariate observations
J. MacQueen (1967)
History and development of the Citrus industry
HJ Webber (1967)
Unexpected Triploids in Citrus: Their Origin, Identification, and Possible Use
A. Esen (1971)
Arabidopsis thaliana triploid response
R Herrero (1971)
Improvement of shoot-tip grafting in vitro for virus-free citrus.
L. Navarro (1975)
Percentages of spontaneous triploids in progenies of diploid lemons and mandarins. International Citrus Congress (2nd, Orlando, Florida), International Society of Citriculture
G Geraci (1977)
Use of sexual tetraploid seed parents for production of triploid citrus hybrids
JW Cameron (1978)
Genetic evidence for the origin of diploid megagametophytes in Citrus
A. Esen (1979)
Seed development in relation to ploidy of zygotic embryo and endosperm in polyembryonic Citrus
A Wakana (1981)
Citrus isozymes Genetics and distinguishing nucellar from zygotic seedlings
A. Torres (1982)
Further evidence for a polymorphism in gametic segregation in the tetraploid treefrog Hyla versicolor using a glutamate oxaloacetic transaminase locus.
R. Danzmann (1983)
Nonselective Phytotoxins Simultaneously Produced with Host-selective ACTG-toxins by a Pathotype of Alternaria citri Causing Brown Spot Disease of Mandarins
Y. Kono (1986)
Alternaria alternata Japanese pear pathotype. Agricultural and Biological Chemistry
S Nakatsuka (1986)
Single-Locus Inheritance in the Tetraploid Treefrog Hyla versicolor with an Analysis of Expected Progeny Ratios in Tetraploid Organisms.
J. E. Marsden (1987)
Investigation and utilization of citrus varietal resources in China
cultivo su uso y (1988)
Intra and intergeneric hybridization in the orange subfamily
M Iwamasa (1988)
Inheritance and Two Medchanisms of 2n Egg Fromation in 2x Potatoes
J. Werner (1990)
Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations.
R. Michelmore (1991)
Colchicine-induced polyploidy in Citrus embryogenic cultures, somatic embryos, and regenerated plantlets
F. Gmitter (1991)
Occurrence and mechanisms of 2n egg formation in 2x potato
J. Werner (1991)
Embryogenesis in Vitro and Nonchimeric Tetraploid Plant Recovery from Undeveloped Citrus Ovules Treated with Colchicine
F. Gmitter (1991)
tangerine pathotype of Alternaria alternata
K Kohmoto (1991)
Eight New Somatic Hybrid Citrus Rootstocks with Potential for Improved Disease Resistance
E. Louzada (1992)
Micro and macro-sporogenesis of two triploid hybrids of citrus
S Fatta Del Bosco (1992)
Molecular data and the dynamic nature of polyploidy
D. Soltis (1993)
Polyploidy is a major component of angiosperms evolution (Grant
Soltis (1993)
based vector for citrus trees. Virology
E Foss (1993)
chiasma interference model
Foss (1993)
Genetics of Powdery Mildew Resistance in Barley
J. Jørgensen (1994)
A detailed RFLP map of cotton, Gossypium hirsutum x Gossypium barbadense: chromosome organization and evolution in a disomic polyploid genome.
A. J. Reinisch (1994)
Citrus. Crops Production Science in Horticulture
FS Davies (1994)
Fruit Characteristics and Pollen Fertility of Citrus Somatic Hybrids
S. Kobayashi (1995)
Half-tetrad analysis in zebrafish: mapping the ros mutation and the centromere of linkage group I.
S. Johnson (1995)
Statistical analysis of crossover interference using the chi-square model.
H. Zhao (1995)
rather than the gametophytic number (Harlan and DeWet
Bretagnolle (1995)
interspecific transferability in Citrus
P e268-73. Ollitrault (1995)
Somatic hybridisation in citrus : some new hybrid and alloplasmic plants
P. Ollitrault (1996)
Methods of Genome Analysis in Plants
P. Jauhar (1996)
Factors influencing infection of onion leaves by Alternaria porri and subsequent lesion expansion
K. Everts (1996)
K. Pegg (1996)
Strategies and considerations in mandarin improvement programmes
A Vardi (1996)
Morphological description of citrus colchiploids
M Jaskani (1996)
Estimation of 2n Megagametophyte Heterozygosity in a Diploid Blueberry (Vaccinium darrowi Camp) Clone Using RAPDs
N. Vorsa (1997)
A comparison of performance of tetraploid progenies produced by diploid and their vegetatively doubled (tetraploid) counterpart parents
G. Tai (1997)
Comparison of FDR- and SDR-derived tetraploid progeny from 2x×4x crosses using haploids of Solanum tuberosum L. that produce mixed modes of 2n eggs
D. Douches (1998)
Postmeiotic restitution in 2n-egg formation of diploid potato
H. J. Bastiaanssen (1998)
A high-resolution linkage map of the citrus tristeza virus resistance gene region in Poncirus trifoliata (L.) Raf.
D. Fang (1998)
J. Ramsey (1998)
Allele frequency distributions in pooled DNA samples: applications to mapping complex disease genes.
S. Shaw (1998)
Phylogenetic relationships within the genus Citrus (Rutaceae) and related genera as revealed by RFLP and RAPD analysis
C. Federici (1998)
Clusters of resistance genes in plants evolve by divergent selection and a birth-and-death process.
R. Michelmore (1998)
Chromosome regions between centromeres and proximal crossovers are the physical sites of major effect loci for yield in potato: genetic analysis employing meiotic mutants.
J. A. Buso (1999)
Mejora genética de cítricos
María José Asins Cebrián (1999)
Synapsis in a natural autotetraploid
D. P. Hauber (1999)
Genetic analysis of apomixis in Citrus and Poncirus by molecular markers
R. García (1999)
Bulked Segregant Analysis Identifies Molecular Markers Linked to Melampsora medusae Resistance in Populus deltoides.
G. Tabor (2000)
Single-nucleotide polymorphism analysis by pyrosequencing.
A. Ahmadian (2000)
Somatic hybridization in citrus: An effective tool to facilitate variety improvement
J. Grosser (2000)
Gene-centromere mapping of 312 loci in pink salmon by half-tetrad analysis
K. Lindner (2000)
Mapping quantitative trait loci for salt tolerance and cold tolerance in Citrus grandis (L.) Osb. × Poncirus trifoliata (L.) Raf. hybrid populations.
G. Moore (2000)
gene expression in maize hybrids
WW Guo (2000)
A general polyploid model for analyzing gene segregation in outcrossing tetraploid species.
R. Wu (2001)
Segregation of Plants with Undeveloped Anthers among Hybrids Derived from the Seed Parent, 'Kiyomi' (Citrus unshiu×C. sinensis)
M. Nakano (2001)
Indeterminate meiotic restitution (IMR): a novel type of meiotic nuclear restitution mechanism detected in interspecific lily hybrids by GISH
K. Lim (2001)
A multivalent pairing model of linkage analysis in autotetraploids.
S. S. Wu (2001)
The diversification of Citrus clementina Hort. ex Tan., a vegetatively propagated crop species.
M. P. Bretó (2001)
Mode of inheritance on the abnormal development of impregnated ovules derived from Mukaku-kishu
H Nesumi (2001)
Rater for spray timing and effects on yield of Murcott tangor
DY Qu (2001)
Comparison between Poncirus and Citrus genetic linkage maps
C. Ruiz (2002)
Interaction between domains of a plant NBS–LRR protein in disease resistance‐related cell death
P. Moffett (2002)
Crossover interference in Arabidopsis.
G. Copenhaver (2002)
Genotyping of Snps in a polyploid genome by pyrosequencing.
A. Rickert (2002)
Agrobacterium-mediated transformation of embryogenic calluses of Ponkan mandarin and the regeneration of plants containing the chimeric ribonuclease gene
D. Li (2002)
Mapping Mendelian Factors Underlying Quantitative Traits Using RFLP Linkage Maps
David BotsteinS’B (2002)
US Patents: USPP 15,461, USPP 15,703, USPP 16,289
ML Roose (2002)
Intraspecifies and intragenus genetic variability. Theoretical and Applied Genetics
I. Aurantioideae (2002)
Understanding mechanisms of novel gene expression in polyploids.
T. Osborn (2003)
Evaluation of the Alter-Rater Model for Timing of Fungicide Applications for Control of Alternaria Brown Spot of Citrus.
Alka Bhatia (2003)
Molecular, ecological and evolutionary approaches to understanding Alternaria diseases of citrus.
K. Akimitsu (2003)
Allelic variation in gene expression is common in the human genome.
H. S. Lo (2003)
Homoeologous Recombination in Interspecific Hybrids of Lilium
K. Lim (2003)
SNP genotyping and molecular haplotyping of DNA pools.
P. Kwok (2003)
Highly parallel SNP genotyping.
J. Fan (2003)
Application of Cleaved Amplified Polymorphic Sequence Method for Analysis of Cytoplasmic Genome among Aurantioideae Intergeneric Somatic Hybrids
S. Lotfy (2003)
Sequence Analysis of a 282-Kilobase Region Surrounding the Citrus Tristeza Virus Resistance Gene (Ctv) Locus inPoncirus trifoliata L. Raf.1
Zhong-Nan Yang (2003)
Construction of two genetic linkage maps in cultivated tetraploid alfalfa (Medicago sativa) using microsatellite and AFLP markers
B. Julier (2003)
Alternaria diseases of citrus - Novel pathosystems.
L. Timmer (2003)
The KBiosciences Competitive Allele-Specific PCR SNP genotyping system [KASPar; (Cuppen, 2007)] is a simple and cost-effective system compared with other
Fan et al (2003)
Review: seedlessness in Citrus
H Raza (2003)
Heterozygosity in 2n gametes of potato evaluated by RFLP markers
A. Barone (2004)
A re-examination of the mechanisms of 2n gamete formation in potato and its implications for breeding
M. S. Ramanna (2004)
Analysis of dihaploid induction and production ability and seed parent x pollinator interaction in potato
R. Hutten (2004)
Cytological evidences of SDR-FDR mixture in the formation of 2n eggs in a potato diploid clone
C. Conicella (2004)
Laboratory Evaluation of Citrus Cultivars Susceptibility and Influence of Fruit Size on Fortune Mandarin to Infection by Alternaria alternata pv. citri
A. Vicent (2004)
Somatic hybrid plants obtained by protoplast fusion between Citrus sinensis and Poncirus trifoliata
T. Ohgawara (2004)
Sexual polyploidization and depolyploidization: some terminology and definitions
A. Mendiburu (2004)
Genome mapping of polyploid tall fescue (Festuca arundinacea Schreb.) with RFLP markers
W. Xu (2004)
QTL analysis of citrus tristeza virus-citradia interaction
M. Asins (2004)
Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture
F Luro (2004)
Sanguinelli’) (Luro et al., 2004) and for C. sinensis × Poncirus trifoliata
‘Tarroco Rosso (2004)
Linkage of an Alternaria Disease Resistance Gene in Mandarin Hybrids with RAPD Fragments
Z. Dalkiliç (2005)
Meiotic nuclear restitution mechanisms in a triploid lily
E. Angela (2005)
Pollination Study of Mandarins and the Effect on Seediness and Fruit Size: Implications for Seedless Mandarin Production
C. Chao (2005)
Polyploidy and evolution in plants.
J. Wendel (2005)
The advantages and disadvantages of being polyploid
L. Comai (2005)
Theoretical and Applied Genetics 109:1125–1132
KB Lim (2005)
The triploid mandarin breeding program in Spain
L Navarro (2005)
Genetics of global gene expression
M. Rockman (2006)
The complete chloroplast genome sequence of Citrus sinensis (L.) Osbeck var 'Ridge Pineapple': organization and phylogenetic relationships to other angiosperms
M. Bausher (2006)
Mapping Novel Traits by Array-Assisted Bulk Segregant Analysis in Saccharomyces cerevisiae
M. Brauer (2006)
Analysis of 13000 unique Citrus clusters associated with fruit quality, production and salinity tolerance
J. Terol (2006)
The Broad-Spectrum Blast Resistance Gene Pi9 Encodes a Nucleotide-Binding Site–Leucine-Rich Repeat Protein and Is a Member of a Multigene Family in Rice
Shaohong Qu (2006)
Assessing genetic diversity and population structure in a citrus germplasm collection utilizing simple sequence repeat markers (SSRs)
N. Barkley (2006)
Genotyping pooled DNA using 100K SNP microarrays: a step towards genomewide association scans
E. Meaburn (2006)
Phylogenetic relationships within Citrus and its related genera as inferred from AFLP markers
Xiao-ming Pang (2006)
Variation in Plant Polyploids
C Chen (2006)
Citrus breeding: introduction and objectives.
I. Khan (2007)
A genome-wide SNP panel for mapping and association studies in the rat
I. J. Nijman (2007)
Origin and taxonomy.
E. Nicolosi (2007)
Genomic DNA pooling for whole-genome association scans in complex disease: empirical demonstration of efficacy in rheumatoid arthritis
S. Steer (2007)
Citrus germplasm resources.
R. Krueger (2007)
S. (2007)
Seedlessness and ploidy manipulations.
P. Ollitrault (2007)
Studies on the Formation and Transmission of n + 1 Gametes of Cabbage Primary Trisomics
C. Zhang (2007)
Cytogenetics. In: IA Khan, ed. Citrus Genetics, Breeding and Biotechnology
MJ Jaskani (2007)
Characterization of microsatellite markers in Citrus reticulata Blanco
Y Froelicher (2007)
SNP assays in allotetraploid cotton
X Cai (2007)
New citrus cultivar "Tsunokaori
Gulsen et al., 2010), the resistant phenotype corresponds to immune symptom
Reis (2007)
Variation in crossover interference levels on individual chromosomes from human males.
J. Lian (2008)
A chromosome-specific estimate of transmission of heterozygosity by 2n gametes in potato.
S. Peloquin (2008)
Advances in molecular marker techniques and their applications in plant sciences
M. Agarwal (2008)
Transferability of the EST-SSRs developed on Nules clementine (Citrus clementina Hort ex Tan) to other Citrus species and their effectiveness for genetic mapping
F. Luro (2008)
Maize centromere mapping: a comparison of physical and genetic strategies.
R. Okagaki (2008)
Breeding for 2n egg production in haploid × species 2x potato hybrids
R. Ortiz (2008)
Tetraploid citrus rootstocks are more tolerant to salt stress than diploid.
B. Saleh (2008)
Pathogen corruption and site-directed recombination at a plant disease resistance gene cluster.
E. Nagy (2008)
Cytogenetical Studies in Genus Citrus IV . Evolution in genus Citrus
S. Raghuvanshi (2008)
Citrus Congress International
A Vardi (2008)
disease: empirical demonstration of efficacy in rheumatoid arthritis. Genes and Immunity
M Stift (2008)
trifoliata and their reciprocal crosses
C Chen (2008)
Congress “Modern Variety Breeding for Present and Future Needs
J Cuenca (2008)
Population genetics of fungal and oomycete effectors involved in gene-for-gene interactions.
E. Stukenbrock (2009)
Global Analysis of Allele-Specific Expression in Arabidopsis thaliana
X. Zhang (2009)
Assessment of the origin of new citrus tetraploid hybrids (2n = 4x) by means of SSR markers and PCR based dosage effects
S. Ferrante (2009)
Relevance of unilateral and bilateral sexual polyploidization in relation to intergenomic recombination and introgression in Lilium species hybrids
N. Khan (2009)
Protectant activity of reduced concentration copper sprays against Alternaria brown spot on ‘Fortune’ mandarin fruit in Spain
A. Vicent (2009)
Turning Meiosis into Mitosis
I. d'Erfurth (2009)
Bulked Segregant Analysis Using the GoldenGate Assay to Locate the Rpp3 Locus that Confers Resistance to Soybean Rust in Soybean
D. Hyten (2009)
The role of hybridization in plant speciation.
P. Soltis (2009)
Unreduced 3x gamete formation of allotriploid hybrid derived from the cross of Primula denticulata (4x) × P. rosea (2x) as a causal factor for producing pentaploid hybrids in the backcross with pollen of tetraploid P. denticulata
M. Hayashi (2009)
Production of tetraploid plants of non apomictic citrus genotypes
P. Aleza (2009)
Evaluation of tangerine hybrid resistance to Alternaria alternata
Marcelo Claro de Souza (2009)
Nucleotide Polymorphism microarrays
A Bérard (2009)
Association of Existing and New Candidate Genes for Anxiety, Depression and Personality Traits in Older People
M. Luciano (2010)
Characterization and mapping of a novel mutant sms1 (senescence and male sterility 1) in rice.
Wenyi Yan (2010)
A new citrus linkage map based on SRAP, SSR, ISSR, POGP, RGA and RAPD markers
O. Gulsen (2010)
Mecanismo del origen de los gametos 2n en el Mandarino Fortune y su implicacion en las estructuras genéticas y phenotypicas de sus poblaciones de hibridos triploides
José Cuenca (2010)
'Garbí' Mandarin: A New Late- maturing Triploid Hybrid
P. Aleza (2010)
Arabidopsis thaliana Chromosome 4 Replicates in Two Phases That Correlate with Chromatin State
Tae-Jin Lee (2010)
Protoplast fusion for production of tetraploids and triploids: applications for scion and rootstock breeding in citrus
J. Grosser (2010)
First Report of Alternaria Brown Spot of Citrus Caused by Alternaria alternata in Yunnan Province, China.
X. Wang (2010)
Model SNP development for complex genomes based on hexaploid oat using high-throughput 454 sequencing technology
R. Oliver (2010)
Role of the host-selective ACT-toxin synthesis gene ACTTS2 encoding an enoyl-reductase in pathogenicity of the tangerine pathotype of Alternaria alternata.
Naoya Ajiro (2010)
The Origin of Cultivated Citrus as Inferred from Internal Transcribed Spacer and Chloroplast DNA Sequence and Amplified Fragment Length Polymorphism Fingerprints
Xiaomeng Li (2010)
‘Safor’ Mandarin: A New Citrus Mid-late Triploid Hybrid
J. Cuenca (2010)
Somatic hybridization for citrus rootstock breeding: an effective tool to solve some important issues of the Mediterranean citrus industry
D. Dambier (2010)
Three of the 2x × 2x crosses share ‘Fortune’ as the female parent, with ‘Willowleaf’ mandarin (93 hybrids), ‘Minneola’ tangelo (127 hybrids) and ‘Murcott
Aleza (2010)
These methods require the analysis of a large set of molecular markers to encounter, by chance, the loci with HR lower than 50% that are only found under SDR
Chen et al (2010)
The triploid mandarin breeding program in Spain. XII International Citrus Congress
L Navarro (2010)
SNP mining in C. clementina BAC end sequences; transferability in the Citrus genus (Rutaceae), phylogenetic inferences and perspectives for genetic mapping
P. Ollitrault (2011)
Development of breeder-friendly markers for selection of MIPS1 mutations in soybean
M. Luciana Rosso (2011)
High-throughput SNP discovery and genotyping in durum wheat (Triticum durum Desf.)
D. Trebbi (2011)
Large changes in anatomy and physiology between diploid Rangpur lime (Citrus limonia) and its autotetraploid are not associated with large changes in leaf gene expression.
T. Allario (2011)
Combining SNP discovery from next-generation sequencing data with bulked segregant analysis (BSA) to fine-map genes in polyploid wheat
M. Trick (2011)
Microarray-based genome-wide association studies using pooled DNA.
S. Szelinger (2011)
Polyploidization mechanisms: temperature environment can induce diploid gamete formation in Rosa sp.
Yann Pécrix (2011)
Unreduced gamete formation in plants: mechanisms and prospects.
L. Brownfield (2011)
Genome-Wide Crossover Distribution in Arabidopsis thaliana Meiosis Reveals Sex-Specific Patterns along Chromosomes
Laurène Giraut (2011)
《Agricultural Sciences in China》近五年载文统计分析
贺文爱 (2011)
High-resolution melting analysis for SNP genotyping and mapping in tetraploid alfalfa (Medicago sativa L.)
Y. Han (2011)
Diploid and Polyploid Gametes in Crop Plants: Mechanisms of Formation and Utilization in Plant Breeding
R. Veilleux (2011)
Evidence for non-disomic inheritance in a Citrus interspecific tetraploid somatic hybrid between C. reticulata and C. limon using SSR markers and cytogenetic analysis
M. Kamiri (2011)
AlleleSeq: analysis of allele-specific expression and binding in a network framework
J. Rozowsky (2011)
Production of viable male unreduced gametes in Brassica interspecific hybrids is genotype specific and stimulated by cold temperatures
A. Mason (2011)
A reference linkage Map of C. clementina based on SNPs, SSRs and indels (P477)
P. Ollitrault (2011)
hybrids by embryo rescue and flow cytometry from 2x x 2x sexual hybridisation and its application to extensive breeding programs
P Aleza (2011)
The NADPH oxidase-mediated production of hydrogen peroxide (H(2)O(2)) and resistance to oxidative stress in the necrotrophic pathogen Alternaria alternata of citrus.
S. L. Yang (2012)
A reference genetic map of Citrus clementina; citrus evolution inferences from comparative mapping.
P. Ollitrault (2012)
Tricking the Guard: Exploiting Plant Defense for Disease Susceptibility
J. Lorang (2012)
Molecular mapping of the downy mildew resistance gene Ppa3 in cauliflower (Brassica oleracea var. botrytis L.)
S. Singh (2012)
S. Sdiri (2012)
Extensive citrus triploid hybrid production by 2x × 4x sexual hybridizations and parent-effect on the length of the juvenile phase
P. Aleza (2012)
A reference genetic map of C. clementina hort. ex Tan.; citrus evolution inferences from comparative mapping
P. Ollitrault (2012)
Location of a chromosome region linked to #Alternaria alternata# pv. Citri resistance from the evaluation of triploid mandarin populations
J. Cuenca (2012)
Use of 2n Gametes in Plant Breeding
A. Dewitte (2012)
Comparative Transcript Profiling of a Male Sterile Cybrid Pummelo and Its Fertile Type Revealed Altered Gene Expression Related to Flower Development
Beibei Zheng (2012)
Phenolic composition, organic acids, sugars, vitamin C and antioxidant activity in the juice of two new triploid late-season mandarins
S. Sdiri (2012)
Implementation of extensive citrus triploid breeding programs based on 4x × 2x sexual hybridisations
P. Aleza (2012)
2012b), and we confirmed SDR at the individual level for most hybrids
Aleza (2012)
polyploids using Competitive Allele-Specific PCR: application to triploid citrus progenies. XII International Citrus Congress
J Citriculture. Cuenca (2012)
markers in mandarin orange (Citrus reticulata Blanco)
Y Froelicher (2012)
formation and centromere mapping in citrus
Valence (2012)
Repellent compositions and genetic approaches for controlling Huanglongbing. WIPO Patent Application WO/2011/050432
B Alquézar (2012)
Citrus genomics. Tree Genetics & Genomes
FG Gmitter (2012)
International Society of Citriculture
P Navarro (2012)
New tangors for Brazilian citriculture
E Schinor (2012)
Two of the 2x × 4x crosses share ‘Orlando 4x’ as the male parent, with ‘Clemenules
Aleza (2012)
late triploid hybrid
P Ollitrault (2012)
megagametophyte production and partial chiasma interference in mandarin cv 'Fortune'. Heredity
J Cuenca (2012)
diploid and polyploid Ipomoea
E Butelli (2012)
formation and centromere mapping in citrus
P. Citriculture. Aleza (2012)
mandarin populations
J Citriculture. Cuenca (2012)
cultivated rye (Secale cereale L.)
CM Lewis (2012)
Screening for Alternaria Brown Spot resistance in the triploid mandarin breeding program in Spain. XII International Citrus Congress
DDM. Bassimba (2012)
Sexual polyploidization in plants – cytological mechanisms and molecular regulation
N. De Storme (2013)
Host-selective toxins produced by the plant pathogenic fungus Alternaria alternata.
T. Tsuge (2013)
The draft genome of sweet orange (Citrus sinensis)
Q. Xu (2013)
Genetic diversity and population-structure analysis of mandarin germplasm by nuclear (SSR, indel) and mitochondrial markers
A García-Lor (2013)
Genome sequencing reveals complex history of admixture
JV Sanz (2013)
Production of host-specific toxins and their biological activity
I. strawberry (2013)
Differences in the genetic structure of citrus triploid hybrids recovered from 2x à 2x and 4x à 2x sexual hybridizations
P. Aleza (2015)
L. Navarro (2015)
Assignment of SNP allelic configuration in polyploids using Competitive Allele-Specific PCR: application to triploid citrus progenies
J. Cuenca (2015)

Semantic Scholar Logo Some data provided by SemanticScholar